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Citations for Promega :
6401 - 6450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... FuGENE HD reagent (Promega, #E2311) was used for the co-transfection ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rRNA region was amplified with the standard bacterial primers 335F and 769R with GoTaq Mastermix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... LUC and REN activities were measured 48 h later in extracts from infiltrated leaf discs using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Cell Titer-Glo Luminescent Cell Viability Assay kit (Promega, G7750) was used for cell viability.
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bioRxiv - Systems Biology 2023Quote: ... the culture medium was removed and 50 µL of 100x diluted NanoLuc substrate (Furimazine, Promega Nano-Glo, N1120) was added to each well of a 96-well microplate containing the transfected cells ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Systems Biology 2023Quote: Genomic DNA was extracted from each cell pellet using the Promega Wizard Genomic DNA Purification Kit (Promega #A1120), following standard protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was then treated with DNAse I (Promega) to remove gDNA contaminant according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 1μg of total RNA was retrotranscribed with Improm II Reverse Transcriptase (Promega). cDNA was mixed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were incubated at 37°C for 96 hours and lysed using CellTiter-Glo Reagent (Promega, catalog. no. G7570). Luminescence was measured using a PHERAstar FS instrument and growth inhibition was calculated relative to DMSO-treated wells.
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bioRxiv - Plant Biology 2023Quote: ... First strand cDNA was synthesised from 500 ng of total RNA using the GoScriptTM Reverse Transcription System and oligo (dT)15 primers (Promega Madison, WI, USA) according to their instructions with the optional addition of RiboLock RNase inhibitor (20 units ...
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bioRxiv - Plant Biology 2023Quote: ... were amplified using GoTaq™ G2 polymerase (Promega corp. Madison WI, USA) according to the manufacturer’s instructions and gene specific primer pairs containing 5’ adaptor sequences (Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... was freshly diluted 1:50 in NanoGlo Buffer (Promega, N112A). For a nano-luciferase assay ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... 25 μL of CellTiter-Glo (Promega, G7573) was added to another 25 μL of lysate ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... cells were lysed with 60 μL Glo Lysis Buffer (Promega, E2661) for 5 min at room temperature with constant rocking ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... NanoGlo Substrate (Promega, N113B) was freshly diluted 1:50 in NanoGlo Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The complexes were washed with RIPA buffer and the samples were treated with DNAse I (Promega) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Capped gRNA was synthesized using either the RiBoMax Large Scale RNA Production Systems (Promega, Madison, Wis, USA) carried out at 30 °C according to protocol or the TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the cap analogue used was Ribo m7G Cap Analog (40 mM; A254 units, Promega, Madison, Wis, USA, P171B). After synthesis ...
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bioRxiv - Microbiology 2023Quote: ... RNA quantity was measured with a QUANTUS Fluorometer (Promega, Germany). Library preparation and sequencing was performed as described in the section “DNA sequencing and hybrid genome assembly” for the short-read library preparation ...
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bioRxiv - Microbiology 2023Quote: ... then harvested for genomic DNA using the Wizard® Genomic DNA Purification Kit (Promega). Genomic DNA was sent for whole- genome sequencing at the Mutualized Platform for Microbiology at Institut Pasteur.
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bioRxiv - Microbiology 2023Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity using a CellTiter-Glo assay (CTG, Promega) at 48 hours post treatment ...
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bioRxiv - Microbiology 2023Quote: ... and so proteins were instead digested using sequencing grade gluC (Promega Corporation) added to the solution in a 1:20 mass ratio ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... and digested by 10 ng trypsin (Promega) at 37 °C for 60 min ...
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bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
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bioRxiv - Systems Biology 2023Quote: ... and digested with Trypsin Gold (Promega, Madison, WI). Azo extracts were irradiated by UV light at 305 nm for 5 min to degrade the photocleavable surfactant ...
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bioRxiv - Systems Biology 2023Quote: ... Dual luciferase reporter activity was measured using the Nano-Glo Dual-Luciferase Reporter assay (Promega, N1630). The luciferase activity data shown is relative to the control pGL4.S4 luc TK activity ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were incubated for one hour at 47 oC with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (w/w ...
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bioRxiv - Zoology 2023Quote: ... then ligated to pGEM-T easy vector (Promega, USA) prior to transforming Escherichia coli-DH5α (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmids were isolated using a PureYield Plasmid Mediprep kit (Promega).
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of extracted RNA was treated with RQ1 RNase-Free DNAse (Promega), then used for cDNA synthesis using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: The HLB1 coding sequence corresponding to the N-terminal 200 amino acids was amplified by PCR using the following primers and was cloned into pGEM-T-easy vector (Promega). The NdeI and XhoI fragment of HLB11-200 was cloned into pET28a vector (Novagen ...
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bioRxiv - Pathology 2023Quote: Drug cytotoxicity was measured by the lactate dehydrogenase (LDH)-GloTM Cytotoxicity Assay Kit (#J2380, Promega) following manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The GoTaq G2 Polymerase (Promega) was used for genotyping PCR using PCR primers as listed in Table S1.
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bioRxiv - Pathology 2023Quote: ... Luminescence was measured at 400 nm using the GloMax® Explorer Multimode Microplate Reader (#GM3500, Promega).
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bioRxiv - Biochemistry 2023Quote: ... was obtained from the Nano-Glo Luciferase Assay System kit (Promega, UK) provided at a concentration of 5 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NanoBRET NanoGLO substrate (Promega) was diluted 83.3-fold in Opti-MEM without FBS and 10 µL were added to each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... gel pieces were dried again and incubated with a freshly prepared solution of 12.5 ng/µl trypsin (sequence grade, Promega) in 50 mM Ambic for 16-20 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Molecular biology enzymes and reagents were from Promega (Southampton, UK). TUG-891 and docosahexaenoic acid (DHA ...
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bioRxiv - Synthetic Biology 2023Quote: ... backbone to contain the large or small fragments of the NanoBiT luciferase (Large BiT or Small BiT) used for the split luciferase complementation assay (Promega, Madison, WI, USA)38 ...
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bioRxiv - Microbiology 2023Quote: ... USA) was performed using primer 27F on a V1-V3 16S rRNA gene colony-PCR amplicon (GoTaq Green, Promega) of primers 27F and 519R ...
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bioRxiv - Microbiology 2023Quote: ... a plasmid serving as the template for homologous recombination of pCARD into the T4 genome was created by cloning the pCARD gene between 50 bp recombination overhang sequences (ordered from Twist Biosciences) in the pGEM-9Zf plasmid (Promega P2391). The recombination overhangs targeted the pCARD gene for insertion after the predicted promoter of the T4 IPI gene38 ...
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bioRxiv - Microbiology 2023Quote: ... RNA quantity was assessed with a QUANTUS Fluorometer (Promega, Germany). Library preparation and sequencing was done as described in the section “DNA sequencing and hybrid genome assembly” for the short-read library preparation ...