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Citations for Promega :
6251 - 6300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... PCR products were purified via gel excision and column purification (Promega, A9282), followed by insertion into the BsmBI–digested lentiV2-hU6-evopreQ1 vector by Gibson assembly (NEB ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction was then performed with the Wizard® Genomic DNA Purification Kit (Promega). Digested samples were centrifuged at 18,000 x g at 4 °C for 2 minutes and the supernatant discarded ...
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bioRxiv - Genetics 2023Quote: ... cells were harvested and assayed using Dual-Glo® luciferase assay system (Promega). The luciferase activities were measured using luminescence reader (Mplex (Infinite M200) ...
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bioRxiv - Genetics 2023Quote: CellTiter 96 AQueous One Solution (Promega G3580) was added to fresh BM-MSC complete growth media ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with a total of 4.5 µg plasmid DNA using Fugene HD (Promega): 0.25µg envelope vector pMD2.G (RRID:Addgene_12259) ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing purity, Promega), as previously described [51] ...
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Genetics 2023Quote: The input material for Cas9-directed LRS was the remaining Bionano-isolated ultra-high molecular weight DNA or Maxwell RSC (AS1400) (Promega, Madison, WI) or Nanobind CBB (Pacific Biosciences ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was isolated using the Promega Wizard gDNA kit (A1120; Promega) as directed ...
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bioRxiv - Genomics 2023Quote: ... using the Maxwell® 16 LEV plant RNA kit (Promega). The RNA concentration was measured by Nanodrop (Thermofisher) ...
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bioRxiv - Genomics 2023Quote: ... According to the manufacturer’s protocol (Promega), RNA was reverse transcribed to cDNA firstly with random hexamers (50 ng/µl ...
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bioRxiv - Genomics 2023Quote: ... and then RNA isolation was done by Maxwell® AS2000 (Promega) using the Maxwell® 16 LEV plant RNA kit (Promega) ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was extracted using the Wizard SV genomic DNA purification kit (Promega). Virus titer and copy number of viral particles per cell were measured by qPCR as previously described [22].
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bioRxiv - Genomics 2023Quote: ... The IL experiment RNA isolation was conducted using the Maxwell® 16 LEV plant RNA kit (Promega) (35) ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted using Wizard® Genomic DNA Purification Kit (Promega, A1120). After measuring DNA quantity by Qubit (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... And was then treated with DNase I (Promega). First-strand cDNA was then synthesized using M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Genetics 2023Quote: ... 0.2% SDS (Promega) and 1 mg/ml Proteinase K (Merck ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: ... We used lipofectamine 2000 to transfect 106 NIH-3T3 MEFs with 3 μg of the luciferase plasmid with a WT DBS or its mutant counterpart and 30 ng of a renilla luciferase expression plasmid (pGL4.73, Promega) for normalization ...
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bioRxiv - Biochemistry 2023Quote: ... was from American Radiolabeled Chemicals Inc. (St. Louis, MO, USA) and PCR reagents and restriction enzymes from Promega Corporation (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Biochemistry 2023Quote: ... and real-time quantitative PCR experiments were performed using SYBR Green (Promega) in CFX connect Real-Time PCR detection system (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: TPCK-treated sequencing-grade modified trypsin was obtained from Promega (Madison, WI). UltraPure™ Tris Buffer was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2023Quote: GsaB-modified GsaA precursor protein was digested with trypsin (Promega, sequencing-grade) at 37 °C for 20 min. ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Genetics 2023Quote: ... Purified RNA was treated with DNase (Promega) at 37°C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... 1μl of random primers (Promega), RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Cell Biology 2023Quote: The supernatants of cells were collected and then applied to cytotoxicity test using CytoTox 96® Reagent (Promega) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... Mass spectrometry-grade LyC (Promega, Madison, WI) was added at an enzyme-to-protein ratio of 1:50 (w/w ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Luciferase and renilla activities were measured with a dual-LUC Reporter Assay System (Promega, Madison, WI, USA) after 2 days of incubation in the dark and 1 day of cultivation under normal growth conditions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase inhibitors (Promega N2615 and Life Technologies AM2696)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA was synthesized for each sample using a GoScript™ Reverse Transcriptase kit (Promega, Madison, WI, USA). The Ischnura-specific RL14 and GADPH gene sequences were annotated as described above for opsin sequences and used as reference for mRNA quantification.
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bioRxiv - Cell Biology 2023Quote: ... and cDNA synthesis using the GoScript Reverse Transcription Mix Random Primers (A2800, Promega) were done according to the instructions of the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The activity of the two luciferase enzymes was measured 24 h after co-transfection following the manufacturer’s instruction of Dual-Luciferase® Reporter Assay System (Cat# E1910, Promega, Wisconsin, USA). Nine biological replications were carried out for each transfection.
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bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 25 mM MgCl2 (Promega), 10 µM P7/P8 and either 50 µM oxo- dGTP or 1 µM dPTP (Jena Bioscience ...
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bioRxiv - Genetics 2023Quote: ... we cloned the 300 bp region, rs35907548 at the middle (chr12:129,282,013-129,282,312, hg 19) locus into pGL4.26 (Promega) and co-transfected with pGL4.74 (internal control ...
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bioRxiv - Genetics 2023Quote: ... as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega, Wisconsin, Madison, United States). In addition ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
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bioRxiv - Genomics 2023Quote: ... A simple cloning vector called pGEM-T was used for cloning (Promega, Madison, WI, USA). After that ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was done using cDNA ImProm-II reverse transcription kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Genetics 2023Quote: ... Fragments were inserted in reporter vector pGL3-Basic Vector (Promega, Cat. No. E1751) upon XhoI and MluI-HF enzymes digestion (New England BioLabs ...
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bioRxiv - Genetics 2023Quote: ... Luciferase intensity was measured 24 hours after transfection with Dual-Luciferase® Reporter Assay System (Promega, Cat. No. E1910) according to manufacture procedure in Orion L Microplate Luminometer with a 96 well plate (Corning Costar plate Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-methionine-labeled Ctb or its derivatives were produced in coupled in vitro transcription and translation reaction (IVTT, at 30 °C for 1 h) using TNT Quick Coupled Transcription/Translation System (cat#L1170, Promega, Madison, WI, USA). Prey proteins were diluted in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells collected by LCM (29,50) and control genomic DNA (gDNA) (Promega), sequenced using conventional sequencing methods (13).
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bioRxiv - Bioengineering 2023Quote: ... and reverse transcribed into complementary DNA with Gotaq Reverse Transcriptase (Promega) using a reverse primer matching on the 3’and of viral mRNA ...
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bioRxiv - Bioengineering 2023Quote: ... RNA samples prepared were treated with DNase according to the manufacturer’s instructions (RQ1 RNase-Free DNase from Promega), quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...