Labshake search
Citations for Promega :
6201 - 6250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fast Break lysis solution (Promega, Madison, WI) was included as positive control.
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bioRxiv - Microbiology 2023Quote: ... for 20min at room temperature under shaking (250rpm) and luminescence was quantified with the Renilla Luciferase Assay System (Promega).
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using Renilla lysis buffer (Promega) for 20min at room temperature under shaking (250rpm ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM DTT) buffer and luminescence activity was measured with the Luciferase Assay System (Promega).
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bioRxiv - Microbiology 2023Quote: ... using PLB buffer and luminescence activity was measured with the Nano-Glo® Luciferase Assay System (Promega).
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: ... a 1-1.5 kb fragment of the promoter containing at its center the putative DBS was cloned upstream a luciferase reporter gene in the backbone of a PGL3 basic vector (Promega). For all tested DBS ...
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bioRxiv - Genetics 2023Quote: ... GoTaq DNA Polymerase (Promega, #M3008) and 1µL of template gDNA ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Genetics 2023Quote: Analysis System (Promega) is recorded ...
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bioRxiv - Genetics 2023Quote: ... The EDNRB2 amplicon were cloned into pGEM-T Easy (Promega) and confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... which was further cloned into pGL4.23 luciferase reporter vector (Promega) at the KpnI/HindIII sites ...
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bioRxiv - Cell Biology 2023Quote: ... CytoTox 96 LDH-release assay kit (G1780) was from Promega. Efficient eukaryotic transfection reagent VigoFect was purchased from Vigorous Biotechnology (Beijing) ...
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Microbiology 2023Quote: ... Samples were incubated with 50 μg of sequencing grade modified trypsin (Promega) and incubated at room temperature with rotation for 18 hr ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and measured for luciferase activities using the Dual luciferase reporter assay system (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected and measured for luciferase activities using the Dual luciferase reporter assay system (Promega). Signals were determined in the luminometer (Wallac 1420 VICTOR multi-label counter ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 before measurement of luciferase activity using Nano-Glo® Luciferase Assay (Promega, Cat # N1110) and Tecan Spark plate reader.
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 before measurement of Renilla and Firefly luciferase activities using Dual-Glo® Luciferase Assay System (Promega, Cat #E2920) and Tecan SPARK plate reader ...
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bioRxiv - Microbiology 2023Quote: ... 36.5 µL nuclease-free water (Promega, Madison, WI, USA), 1 µL DNA template (20 ng/µL ...
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bioRxiv - Microbiology 2023Quote: ... using the Dual Luciferase Reporter Assay System (Promega). Frameshift efficiency was determined by the ratio of Firefly luciferase to Renilla luciferase.
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bioRxiv - Microbiology 2023Quote: ... The fused PCR fragment was then cloned into the pGEM-T Easy vector (Promega, Madison, WI) generating flgE::kan ...
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bioRxiv - Immunology 2023Quote: ... The assay was performed at 16hr post infection using the CellTox Green Cytotoxicity Assay Kit (Promega). Fluorescence readings were recorded as triplicates using a Tecan Infinite M1000 Quadruple Monochromator Microplate Reader ...
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bioRxiv - Microbiology 2023Quote: ... HiBiT expression was measured by the HiBiT lytic assay (Promega). To test off-target effects of siRNAs ...
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bioRxiv - Microbiology 2023Quote: ... HCV Nluc was measured by NanoGlo assay (Promega). To determine the effects of vcircRNAs on HCV replication ...
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bioRxiv - Microbiology 2023Quote: ... Samples were digested with 2 μg of trypsin (Promega, Madison, WI, USA) at 47°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... at 16 h post-infection and the cells were then continuously cultured for another 48 h before being subjected to a luciferase assay (Promega Bright-GloTM Luciferase Assay System). The percentage of inhibition was calculated as the ratio of the loss of luciferase readout (RLU ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was measured using the CellTiter-Blue Assay (Promega) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... cells were lysed with passive lysis buffer (Promega) and luciferase activity quantified using a luminometer (TriStar2 LB 942 Multimode Reader ...
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bioRxiv - Microbiology 2023Quote: ... luciferase assays were performed on fecal materials using Nano-Glo luciferase assay kit (Promega) as previous described 28 ...
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bioRxiv - Cell Biology 2023Quote: ... After overnight digestion with Trypsin/Lys-C mix (Promega), peptides were transferred ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using a 2x GoTaq PCR master mix (Promega), biotin-labeled M13F and biotin-labeled M13R primers ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then harvested at 48 hr post infection for measuring NanoLuc activity (Promega). The number of spike trimers per virion was estimated using the following formula ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.33 units of RNasin (Promega), and 10 μl of nuclear extract (25 - 40 μg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... SiGMV and TYLCV were cloned in pFN2A vector (Promega) using SgfI and PmeI restriction enzymes to express them as fused proteins with N-terminal GST tag ...
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bioRxiv - Molecular Biology 2023Quote: ... and western blue stabilized substrate for alkaline phosphatase (Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... with 1X protease inhibitor cocktail (Cat#G6521, Promega, USA) and incubated at 4□C with gentle shaking for 1.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of OMV production in 96-well plate format was done by performing Nano-Glo assays using the Nano-Glo Live Cell Assay System (Promega). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... was determined using ONE-Glo Luciferase Assay System (Promega). Luminescence was read on Infinite F200 pro system (Tecan) ...
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bioRxiv - Microbiology 2023Quote: ... the cells were lysed in 100 μl of One-Glo Luciferase Assay Reagent (Promega). Firefly luciferase activity was determined with Infinite F200 pro system (Tecan).
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bioRxiv - Evolutionary Biology 2023Quote: ... treated with RQ1 RNase-free DNase I (Promega) and purified using the RNAeasy kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed using the 2x GoTaq qPCR master mix (Promega, Walldorf, Germany) with 10 ng of cDNA template and 0.5 µM of each oligonucleotide in a final volume of 15 µL ...
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bioRxiv - Microbiology 2023Quote: GoTaq DNA polymerase kits (Promega) were used for PCR reactions ...
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bioRxiv - Neuroscience 2023Quote: ... or 50 nM) in buffer A with 0.4 U/µL RNase inhibitor (RNasin Plus RNase Inhibitor, Promega). Biotinylated (AAAAAA)4 and TERRA are negative and positive controls ...
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bioRxiv - Immunology 2023Quote: ... Streptavidin alkaline phosphatase conjugate (Promega, cat.: V5591) was diluted 1:2 500 and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and 18 μl of Fugene HD (Promega) to 300 μl of optiMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... or the ATG9A gene using ViaFect (E4981; Promega). Two days after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase assays were carried out using the Dual-Luciferase Kit (Promega) according to manufacturer’s instructions and as detailed in [14].
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bioRxiv - Molecular Biology 2023Quote: ... pGL3-Vλ1p was constructed by cloning the Vλ1 promoter (chr16: 19084509-19085159) in front of luciferase reporter gene in pGL3-Basic (Promega). To construct pGL3-Vλ1p-HSE-1 and pGL3-Vλ1p-HSCλ1 ...