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Citations for Roche :
3051 - 3100 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... the SYBR Green I Master mix was used on a LightCycler 480 Real Time PCR System (Roche). The primers are listed in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were carried out using KAPA HiFi HotStart DNA polymerase from KAPA Biosystems (KAPA Biosystems, Boston, USA). The resulting PCR products were purified using the DNA Gel Extraction Kit from New England Biolab (New England Biolab ...
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bioRxiv - Microbiology 2024Quote: ... PCR was carried out using the KAPA HiFi HotStart Ready Mix (Cat. No. 7958935001, Roche, Basel, Switzerland). The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Real-time qPCR was performed using a LightCycler®480 Real-Time PCR system (Roche Diagnostics, USA) with the LightCycler® 480 SYBR Green I Master Mix (2X ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was performed with LightCycler 480 SYBR Green I Master detection kit (Roche 04707516001) on a Light Cycler 480 machine (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexed libraries were quantified by qRT-PCR using a commercially available kit (Roche KAPA Biosystems Cat# KK4854). The quality of cDNA samples was verified with a bioanalyzer (Agilent Technologies 2100).
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bioRxiv - Cell Biology 2023Quote: • PCR #1: 10 μl reactions were prepared for each sample with KAPA HiFi HotStart ReadyMix (Roche, KK2602) containing 1 μl cDNA template and 1.5 μl of 10 μM PCR1 primer mix (U6 outer ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR (qPCR) was carried out in the LightCycler 480 II system (Roche, Basel, Switzerland) using Universal ProbeLibrary (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... beads were resuspended in 14 μl indexing PCR Mix containing 1x KAPA Hifi HotStart Ready Mix (Roche) and 700 nM unique dual indexing primers (i5 and i7) ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction consisted of 12.5 μl KAPA 2x HiFi Master Mix (KAPA Biosystems, Wilmington, MA, USA), 0.5 μl of 10 μM forward primer ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were amplified using 18 PCR cycles and quantified using KAPA qPCR Library Quantification Kit (KAPA Biosystems) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and qRT-PCR was performed on a Roche 480 Lightcycler with KAPA SYBR FAST (Kapa Biosystems KK4610) after plate loading with an Echo 550 liquid handler ...
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bioRxiv - Genomics 2023Quote: Mastermix 3 (IS-PCR) was freshly prepared and contained 12.5 μl Kapa HiFi Hotstart Readymix (2x, Roche), 0.25 μl IS PCR Primers (10 μM ...
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bioRxiv - Molecular Biology 2023Quote: cDNA samples were analyzed by qPCR using a Light Cycler®96 Real-Time PCR System (Roche) and the qPCR BIO SyGreen Master Mix (PCR Biosystems ...
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bioRxiv - Genomics 2023Quote: ... Real-time qPCR was performed using the Roche LightCycler 480II Real-Time PCR System (Roche, Basel, Switzerland) with the SYBR Green PCR Master Mix ...
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bioRxiv - Cancer Biology 2023Quote: The beads were supplemented with a PCR mix comprising 20 μl of 2× HotStart Readymix (Kapa Biosystems), 4 μl of 5′ end biotin-modified P7 primer (10 μM ...
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bioRxiv - Genomics 2022Quote: ... Gene expression was measured through quantitative real-time PCR (qPCR) using FastStart SYBR Green master mix (Roche) according to the manufacturer’s recommendations in a LightCycler 480 II device (Roche) ...
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bioRxiv - Genetics 2023Quote: ... We PCR-amplified bisulfite-treated DNA with 15-16 cycles with KAPA HiFi Uracil polymerase (Kapa Biosystems). We used Illumina TruSeq unique index adapters for PCR amplification and multiplexing of the libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001). PCR amplification was performed with the following parameters as described in the EpiCypher CUT&RUN kit ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed in 96-well format using the quantitative real-time PCR System (Roche 480 LightCycler). 2 μL cDNA were added to a final reaction volume of 10 μL containing H2O ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time PCRs were carried out with the Fast Start Essential DNA Green Master kit (06402712001, Roche) and Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexed libraries were quantified by qRT-PCR using a commercially available kit (Roche KAPA Biosystems Cat# KK4854). The quality of cDNA samples was verified with a bioanalyzer (Agilent Technologies 2100).
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bioRxiv - Molecular Biology 2023Quote: ... CFP/GFP and mCherry probes were synthesized using the PCR digoxigenin probe synthesis kit (1163090910, Roche, Switzerland) with primers shown in Table 1 and the following PCR conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... DIG-labeled TER and alpha-tubulin probes were generated using the PCR DIG Probe Synthesis Kit (Roche) and used in the hybridization step (S1 Table ...
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bioRxiv - Cancer Biology 2023Quote: ... Full length cDNAs were amplified for 22 cycles of PCR using KAPA HiFi DNA polymerase (KAPA Biosystems). cDNA samples were purified with Ampure XP beads at a 1:0.8 ratio and cDNA quality in randomly selected 10 wells/plate was assessed on a High Sensitivity Bioanalyzer chip (Agilent) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in 10 µL reactions using KAPA SYBR Fast qPCR Kit (KAPA BIOSYSTEMS). Expression levels were calculated using the 2–ΔΔCt method ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative PCR was performed with LightCycler Fast Start DNA Master SYBR Green I (06924204001, Roche, Basel, Switzerland) in LightCycler 96 instrument (Roche ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR profiles were visualized using the commercially available FastStart Universal SYBR Green Mastermix (Roche, SUI) and Qiagen human Primer Assays (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was performed on a Roche LightCycler 480 using KAPA SYBR FAST qPCR MasterMix (Roche: KK4611).
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bioRxiv - Microbiology 2023Quote: ... Polymerase Chain Reactions (PCR) were performed using KAPA HiFi HotStart Ready Mix® (KAPA biosystems, Boston, USA) with the following thermal parameters ...
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bioRxiv - Cell Biology 2024Quote: ... The final pooled library was quantified using a KAPA qRT-PCR Library Quantification assay (Kapa Biosystems, 07960140001). RNA-seq libraries were sequenced on a NextSeq 2000 platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR reactions were performed in 10 µl with Kapa SYBR Fast qPCR universal mix (Kapa Biosystems) with 100 nM of each PCR primer per reaction in a Roche LightCycler 96 with the following program ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ligated fragments were amplified for 16 cycles using KAPA Hifi Hot Start PCR kit (Roche, Basel, Switzerland), and purified again with 1X AMPure XP beads ...
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bioRxiv - Molecular Biology 2024Quote: ... in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche, Basel, Switzerland). Two primers were used in the PCR reaction for assessment of target rDNA expression ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA samples were analysed by qPCR using a Light Cycler®96 Real-Time PCR System (Roche) and qPCR BIO SyGreen Master Mix (PCR Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR reactions were performed in 96-well plates on a LightCycler 96 System (Roche, Basel, Switzerland) using FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was purified from 106 infected cells using the High pure PCR template preparation kit (Roche) and subjected to quantitative real-time PCR (qPCR) ...
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bioRxiv - Plant Biology 2024Quote: ... Gene expression was measured by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics) using the SYBR Premix Ex Taq (Tli RNaseH ...
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bioRxiv - Genetics 2024Quote: ... TaqMan real-time polymerase chain reaction assays (PCR) consisted in 2.5 ul of Fast Master Mix (Roche), 0.125 ul of assay ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed in a 25 μl reaction mixture containing 12.5µL 2X KAPA HiFi HotStart ReadyMix (Roche), 50 ng genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR was performed using the KAPA SYBR FAST ABI Prism 2x qPCR Master Mix (Roche, Switzerland) and a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The SYBR Green Dye detection system was used for quantitative real-time PCR on Light Cycler 480 (Roche). Controls consisting of ddH2O were negative for target and housekeeping genes ...
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bioRxiv - Genetics 2021Quote: ... cDNA was used as a template for quantitative PCR reactions using the FastStart Essential DNA Green Master (Roche) and transcript-specific primer pairs (Table S1 ...
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bioRxiv - Neuroscience 2021Quote: The genotype of these knockout medaka was examined by using PCR with LightCycler 96 (Roche Diagnostics, Basel, Switzerland). The genome DNA of each fish was extracted through proteinase K treatment (0.4 mg/ml proteinase K (Kanto Chemical Co. ...
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bioRxiv - Physiology 2022Quote: Real time quantitative PCR amplification reaction were carried out in a LightCycler 480 detection system (Roche, Basel, Switzerland) using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 1st round PCR was performed by adding 25 μl 2x KAPA HiFi HotStart Ready Mix (KAPA biosystems) with primers of NEB Oligos kit ...
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bioRxiv - Developmental Biology 2022Quote: ... Q-PCR was conducted to detect RNA amounts using FastStart Universal SYBR Green Master (ROX; Roche, Basel, Switzerland) with StepOne Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Size selected cDNA was PCR amplified for 12-14 cycles with KAPA HiFi hot start (KAPA Biosystems, KK4602). Final PCR product was cleaned up with PCI extraction followed by ethanol precipitation and finally separated by 7.5% PAGE-Urea to remove adaptor dimers ...
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bioRxiv - Microbiology 2019Quote: ... The process of qRT-PCR was performed by the LightCycler® 480 SYBR Green I Master (Roche, Germany) in a final reaction volume of 20 μL ...