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Citations for Roche :
3101 - 3150 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: The cell lysates were extracted with RIPA lysis buffer that contained a protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Basel, Switzerland). Equivalent concentrations of protein were separated using gel electrophoresis and were then transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... a debris removal step was performed (Miltenyi #130-109-398) and blood cells were removed using a blood lysis buffer (Roche 11814 389001).
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were harvested 20 hours post-infection (hpi) and suspended in RIPA buffer with protease and phosphatase inhibitors (Roche, Thermo Fisher). Samples containing 10-20 µg protein were boiled for 20 min in sample buffer ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were treated with the oligonucleotide mixed with DOTAP liposomal transfection agent (35 μg/ml) (#11811177001, Roche Diagnostics GmbH, Mannheim, Germany) for 3 to 6 hrs (16) ...
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bioRxiv - Genomics 2021Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) per gram of cells in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Cell Biology 2021Quote: A reference sample was generated by lysing TK6 cells in DPBS with 2% SDS and cOMPLETE protease inhibitors without EDTA (Roche, 1x concentration) at 70 °C ...
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bioRxiv - Microbiology 2020Quote: ... These mutant viruses were made using HEK-293/17 cells that were transfected with 6 μg of viral DNA using Xtreme-GENE HP (Roche, Basel, Switzerland) Transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... an equimolar amount of DMSO was used to stimulate the cells in triplicate wells and as positive controls phytohemagglutinin (PHA, Roche, 1μg/ml) and a cytomegalovirus MP (CMV ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Neuroscience 2021Quote: The culture medium was collected and cells were lysed in RIPA buffer supplemented with 1x cOMPLETE protease inhibitor cocktail (Roche, Basel, Switzerland). Cell lysates and medium for phosphorylated tau and total tau Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Developmental Biology 2022Quote: ... in situ terminal deoxynucleotidyl transferase deoxyuridin triphosphate nick end labeling (TUNEL) assay was performed using in situ cell death kit from Roche (Basel, Switzerland).
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Microbiology 2022Quote: ... genes was performed using reagents and equipment supplied by Advanced Cell Diagnostics (ACDBio, Hayward, CA) and Ventana Medical Systems (Roche, Tuscon, AZ). The in situ hybridization RNAscope® probes where designed based on the simian HAV Macaca/Germany/2021 ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents were calculated according to amplification of the β-globin gene (two copies per diploid cell) with commercially available materials (Control Kit DNA; Roche Diagnostics). 2-LTR circles ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T/17 cells were seeded onto a 6-well plate and then transfected 24 h later using X-tremeGENE HP (Roche, Basel, Switzerland). After transfection (24 h ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first incubated 1 hour with Blocking Buffer (10% heat-inactivated Goat Serum, 0.5% Blocking Reagent [Roche] in PBS-0.5% Tween) and then 1 hour with 1.5U/ml αFAM-POD antibody (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... while remaining cells were harvested in 500 µL lysis buffer (50 mM Tris pH6.8, 2% SDS, 1x protease inhibitor (Roche Complete EDTA-free)) snap-frozen and stored at -70 °C until further processing.
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bioRxiv - Cancer Biology 2024Quote: Terminal dUTP Nick End Labeling (TUNEL) staining was performed with an In Situ Cell Death Detection Kit (TMR Red #12156792910; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were lysed in cold lysis buffer (10mM Tris-HCl pH7.5, 10mM NaCl, 5mM MgCl2, 1mM EGTA, with Roche Protease Inhibitor #04693159001) and incubated on ice for 10 minutes to extract nuclei ...
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bioRxiv - Genetics 2024Quote: ... and the cell pellet was resuspended in 50-200 μl RIPA buffer (volume depending on the pellet size) supplemented with protease (Roche Diagnostics GmbH) and phosphatase inhibitors (Roche Diagnostics GmbH) ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
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bioRxiv - Genomics 2023Quote: ... 100ng of DNA from each cell line was combined and an aliquot of 150ng was fragmented using KAPA Frag Enzyme (Kapa Biosystems KK8514) and KAPA Frag Buffer (10x ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine nick-end labeling (TUNEL) staining was performed by using the In Situ Cell Death Detection Kit from Roche (Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: Cell lysates for intracellular chemokine quantification were obtained via repeated freeze-thaw cycles at −80°C of cells suspended in media containing protease inhibitor cocktail (Roche Applied Science) and final centrifugation at 8000g to pellet debris ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
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bioRxiv - Cell Biology 2023Quote: The cell pellets were washed once in PBS and resuspended in 5 ml PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref. 11684795910, Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Immunology 2023Quote: Min6.luc and Min6.CD44 cells were harvested for total RNA isolation using the High Pure RNA isolation kit (Roche Applied Science) and reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were treated with dilutions of SB939 for 24 or 48 hours and cell proliferation was assessed using ELISA BrdU kit (Roche Diagnostics GmbH), according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... in Tris-EDTA buffer pH 7.8 at 95°C for 64 min in standard cell condition solution one (CC1) using a Ventana Discovery ULTRA (Roche Indianapolis IN). Blocking was then performed in an inhibitor solution for 12 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability of cell lines in the presence of increasing concentrations of different drugs was assayed using the cell proliferation reagent WST-1 (Roche, Mannheim, Germany) after 72 h-treatments as previously reported 35 ...
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bioRxiv - Neuroscience 2024Quote: ... coli bacterial cells were lysed on ice by sonication in Phosphate-Buffered Saline (PBS) supplemented with protease inhibitors (cOmplete, EDTA-free, Roche Applied Science). The protein was purified over pre-packed Glutathione Sepharose High Performance resin column (GSTrap HP columns ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkaline phosphatase staining was conducted on primary OP6 cell cultures on Day-5 post-transfection using NBT/BCIP stock solution (Roche/Sigma 11681451001) or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804 ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time detection of cellular viability was conducted by cellular impedance measurements using the xCELLigence system Real-Time Cell Analyzer RTCA-MP (Roche Diagnostics, Penzberg, Germany) [26] ...
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bioRxiv - Genomics 2020Quote: ... 2 g of DUX4 expression constructs were transfected into RD cells with 6 L of the X-treme GENE 9 DNA transfection reagent (cat. XTG9-RO, Roche Diagnostics, Indianapolis, USA) diluted in 100 L of Opti-MEM I (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We induced transient expression of the plasmids in 106 cells resuspended in 2 ml using the polymer X-tremeGENE 9 DNA Transfection Reagent (Roche Applied Sciences 06365787001) following manufacturer’s instructions (1 μg total DNA and 3 μl reagent in 100 μl FBS-free culture medium) ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-Buffered Saline + 1% Triton X-100 (TBST) with protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Microbiology 2020Quote: ... and 5 µg of a WEAU gp160 plasmid (pcDNA3.1-WEAU gp160) into exponentially dividing 293T/17 cells using FuGENE 6 (Roche Applied Science, Indianapolis, IN) or into 293F cells using 293fectin (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: N2a cells were collected for expression validation from the 12-well plate at 24 h post transfection in RIPA buffer (Cell Signalling Technologies; Catalog No. 9806) in the presence of phosphatase inhibitor (Roche; Catalog No. 04906837001) and protease inhibitor (Roche ...