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Citations for Roche :
2701 - 2750 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid minigenes were transfected 24 h after cell seeding using X-tremeGENE 9 DNA Transfection Reagent (Roche; Mannheim, Germany). HepG2 ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Genomics 2023Quote: ... Cells were washed twice in cold PBS and scraped off the plates in cold PBS containing protease inhibitors (Roche, Complete 11836170001 and Halt ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μg pAcBAC3 plasmid was used to transfect RAW 264.7 cells by mixing the DNA with 6 μg X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of wound tissue were prepared by a combination of enzymatic digestion (Liberase Blendzyme, Roche Applied Science) and mechanical disruption (Medimachine System ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... The TUNEL assay was performed using the in situ Cell Death Detection Kit (11684817910, Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These CPER products were then transfected into Huh7.5 cells using X-tremeGENE™ HP DNA (Roche 6366236001, Basel, Switzerland).
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Flash-frozen cell pellets were resuspended in 1.2 ml RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed as in (46) using the TMR red in situ cell death detection kit (Roche, 12156792910).
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bioRxiv - Neuroscience 2023Quote: Human induced pluripotent stem cell (iPSC) line KYOU-DXR0109B (ATCC) was routinely cultured on growth-factor reduced Matrigel (Roche) in mTeSR1 culture medium (StemCell Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Immunology 2023Quote: ... The final sodium concentrations in the cell culture media were confirmed by potentiometry with a Cobas 8000 analyzer (Roche). T cells were stimulated with plate-bound CD3 (1 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... cell fusion was performed by incubating the dish with 1 ml of prewarmed polyethylene glycol 1500 (PEG 1500, Roche) for 1 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were collected using RIPA lysis buffer (Beyotime Biotechnology, Jiangsu, China) containing 1% protease inhibitor (Roche, 11697498001) and 1% phosphatase inhibitor (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclei were isolated from 5 million cells with hypotonic buffer (20 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, protease inhibitors (Roche)) for 15 min on ice ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were lysed in 300 µL hypotonic lysis buffer (see Vesicle isolation) supplemented with protease inhibitor tablet (Roche). The lysates were split into two and half was treated with cGAMP ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from A549 cells under different treatment conditions using the High Pure RNA Isolation Kit (Roche), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Single cell suspension was obtained by digesting DRGs in Collagenase type 1 and Dispase II (1 mg/ml; Roche) at 37°C for 70 min18 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were generated from minced lung tissue that was subjected to collagenase (Liberase TM Research Grade, Roche), hyaluronidase (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear lysates were prepared by resuspending cell pellets in lysis buffer (10mM Tris pH 8.0, 10mM NaCl, 0.2% NP40) supplemented with protease inhibitors (Roche, # 4693159001) and phosSTOP (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA fragmentation was visualized by using an in situ cell death detection kit (Fluorescein; Cat #, 11684795910; Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
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bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown for 96 h and cellular viability was determined at indicated time points using the cell proliferation reagent WST1 (Roche) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral preparations were prepared using standard techniques by co-transfection of 293T cells with the lentiviral construct together with pMDLg/pRRE and pMD2.G using Fugene (Roche). Culture supernatants were harvested at 72 h after transfection and concentrated by ultracentrifugation using standard techniques ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged and 25 µL of cell-/ debris-free supernatant was used per well for the LDH assay (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: Expression level of Nud1-HA3 and Nud17A-HA3 was determined by western blot analysis of whole cell extracts as previously described (Ten Hoopen et al., 2012) using monoclonal antibody 12CA5 (Roche) at 1:1000 dilution and monoclonal antibody B-5-1-2 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by addition of 5 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (i.e., MTT) using a Cell Proliferation Kit I (Roche Diagnostics, Mannheim, Germany), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Biochemistry 2021Quote: ... or a fixed amount of JR-FL or LAI pseudo-virus (1,000 pg of CA-p24) was added to the cells (70-80% confluency) in the presence of 400 nM saquinavir (Roche) to block secondary rounds of infection and 40 µg/ml DEAE in a total volume of 200 µl ...
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bioRxiv - Developmental Biology 2021Quote: ... The crosslinking reaction was stopped adding Glycine to a final concentration of 0,125 M and the cell pellet was washed three times with cold PBS with protease inhibitors (Complete mini EDTA –free proteinase inhibitor cocktail; Roche). Fixed samples were stored at -80C ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and then washed in PBS buffer before incubation with terminal deoxynucleotide transferase (In Situ Cell Death Detection kit; Roche, Switzerland) for 1 h at 37 °C in a solution containing TMR red dUTP ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell suspensions were prepared by digesting the mouse skin tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...