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Citations for Roche :
2751 - 2800 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the cells or tissues were extracted on ice using standard CHAPS buffer (60 μL per 106 cells) supplemented with a cocktail of protease inhibitors (Roche) and 20 units of RNase inhibitor (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Molecular Biology 2020Quote: ... 100,000 cells were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to activated ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Genomics 2022Quote: Short-insert paired-end libraries of the lrPCR mtDNA product of the cell lines were prepared with KAPA HyperPrep kit (Roche) with some modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with 27 µl labeling solution plus 3 µl enzyme solution (In Situ Cell Death Detection Kit, AP, Roche) at 37°C overnight ...
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bioRxiv - Genomics 2019Quote: ... An additional Proteinase K incubation (65 μl of 10mg/mL per 7 million cells starting material) at 65°C for two hours was followed by RNase A (Roche; 15 μl of 10mg/mL per 7 million cells starting material ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in 500 μl lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.3% NP40, 1× Roche Complete) and kept on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... PBS was aspirated and the cells where lysed in lysis buffer (3x radioimmunoprecipation assay buffer, protease inhibitor cocktail (Roche 1169749801), 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Molecular Biology 2019Quote: Detection of DNA strand breaks in MC3935-treated schistosomula was performed using the In situ Cell Death Detection kit (Roche), as previously described (33) ...
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bioRxiv - Cell Biology 2020Quote: ... washing cells twice with 2mL of cold PBS and lysing in 1mL RIPA buffer with protease inhibitor cocktail (Roche, 4693159001). Collected media was spun down at 400x g for 5 minutes to pellet any floating cells ...
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bioRxiv - Developmental Biology 2019Quote: Embryos were injected with ndr2 RNA (and MOs) at one-cell stage as described above and dechorionated using Pronase (Roche). At 256- to 512-cell stage ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed twice in ice cold PBS and cell pellets were lysed for 10 min at a density of 107 cells/ml in lysis buffer supplemented with Protease inhibitor (Complete Mini EDTA-free Protease Inhibitor cocktail tablets; Roche). Lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The infected cells were harvested in RIPA buffer solution supplemented with cOmplete™ and EDTA-free Protease Inhibitor Cocktail (Roche). Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: Protein lysates from adult zebrafish tissues and transfected HEK293T cells were prepared using RIPA lysing buffer with EDTA-free protease inhibitor (Roche). Concentration of protein lysates was determined using either the Pierce BCA protein assay kit (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... The cytotoxic potential of the samples was determined from THP1-cell supernatants after 3 hours by using the Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and co-transfected into 60-70% confluent BHK cells grown in 6-well plate tissue culture plates using Fugene HD transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells became 70-80% confluent and were transfected with the corresponding plasmids using the transfection reagent FuGENE 6 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: PFA fixed zebrafish larvae were used in combination with the In situ Cell Death Detection Kit (TMR and Fluorescein) (Roche). After O/N fixation larvae were washed and permeabilized for 45 min at 37°C using Proteinase K ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... subtilis strains at the different time points were evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) using the In-Situ Cell Death Detection Kit with fluorescein (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The cDNA encoding for the A33 construct was obtained by total RNA extraction from the HT-29 colorectal carcinoma cell line (High Pure RNA Isolation Kit, Roche), reverse transcription (Transcriptor reverse transcriptase ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 200 mM NaCl, Roche protease inhibitor cocktail) and lysed by two passes through an EmulsiFlex-C5 homogenizer (Aventis) ...
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bioRxiv - Cancer Biology 2021Quote: ... The candidate cells were selected with 2 µg/ml puromycin for a week and the selected cells were analysed by High-resolution melting peaks analysis using real-time PCR (Light cycler 480 II, Roche).
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bioRxiv - Cell Biology 2019Quote: ... TUNEL staining was performed on testis sections according to the instructions provided with the cell death detection kit (11684795910, Roche). To reduce experimental variations ...
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bioRxiv - Microbiology 2021Quote: ... each tube contained about 106 of cells in 200 μl of the digestion buffer containing 50 μU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... Transfected HEK29T cells and COS-7 cells were washed and lysed with 1% Triton X-100 in PBS with protease inhibitor cocktail (Roche) at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with the TUNEL reaction mixture according to the protocol (In situ Cell Death detection Kit, TMR red; Roche) and incubated for 1 h at 37°C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular protein was isolated from exponentially growing cells and lysed in ice cold RIPA buffer containing 1x cOmplete Mini Protease Inhibitor Tablets (Roche). Protein concentration were quantified and 75 ug resolved (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately eight million cells were harvested for each sample and lysed into Pierce lysis buffer (Thermo) containing Complete protease inhibitors (Roche). Lysate was clarified by centrifugation at 10,000 g for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... At 80% confluency the cells were harvested on ice by scraping and washed once with PBS containing protease inhibitors (Roche). The cells were pelleted at 300g for 5 min at 4°C and were washed once with KPBS buffer (136 mM KCL ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid NL4.3 and various mutants were transfected into JLTRG-R5 cells with 1 μg of viral DNA using Xtreme-GENE HP (Roche) Transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysate was prepared by sonication of 2×108 cells on ice in 100 mM sodium phosphate buffer pH 7 including EDTA-free Complete Protease Inhibitors (Roche) and 0.01% TX-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were briefly washed in ice-cold PBS and lysed in RIPA buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2020Quote: Damage to the human colon epithelial cell line HT-29 was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared in 2× Laemmli loading buffer supplemented with cOmplete protease inhibitor and PhosSTOP phosphatase inhibitor tablets (Roche), 1 mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then lysed using 1x RIPA buffer (Thermo) supplemented with 3x HALT Protease inhibitors (Thermo) and 1 × PhosSTOP (Roche), 2 μl (500 Units ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Cancer Biology 2020Quote: ... and lysed in RIPA buffer (Cell Signalling Technology, USA) supplemented with protease (Mini, EDTA-free Protease Inhibitor Cocktail, Roche, UK) and phosphatase (PhosSTOP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells lysed with lysis buffer were incubated in the buffer containing protease and phosphatase inhibitor cocktails (04693159001 and 04906845001, Roche), 1 mM serine protease inhibitor PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates were prepared from melanocyte cell lines using ice-cold RIPA buffer supplemented with complete protease inhibitor cocktail and PhosStop phosphatase inhibitor cocktail (Roche). For western blotting ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells were lysed in 300 μl Triton X-100 lysis buffer supplemented with EDTA-free protease inhibitor mix (Roche). 100 μl supernatant and 100 μl diluted cell lysates were independently incubated with 100 μl AP substrate p-nitrophenyl phosphate (PNPP ...