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Citations for Roche :
2801 - 2850 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 50 μl mix of 5 μl enzyme and 45 μl labeling solution (TUNEL In situ Cell Death Detection Kit, TMR Red’ from Roche) for 1.5 h at 37°C in a dark humid chamber ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Detection of apoptotic cells in tissue sections was performed by the TUNEL assay with the In-Situ Cell Death Detection Kit (TMR red, Roche), according to the protocol described previously22 ...
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bioRxiv - Cell Biology 2022Quote: ... were isolated from whole blood by first centrifugation then the buffy coat was lysed with Red Blood Cell (RBC) Lysis Buffer (Roche) and then washed with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: CD14 negative PBMCs were cultured at 5 × 106 cells/ml in RPMI 1640 with 10% FBS in the absence or presence of Phytohemagglutinin (PHA) (1 μg/ml) (Roche) for 24 h.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Pathology 2022Quote: Total RNA was purified from cells using a Tiangen Easy Fast RNA Extraction kit and reverse-transcribed using Transcriptor First Strand cDNA Synthesis kit (Roche). Expression of mtDNA encoded genes were determined by quantitative real-time RT-PCR using SYBR Green PCR Master Mix (Roche Applied Science) ...
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bioRxiv - Microbiology 2022Quote: ... UK) at 1kbar (10g of cells in 40ml final of 20mM Tris pH 8.0 containing protease inhibitor (Roche, EDTA free), at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: Pellets were lysed via sonication of a 33 percent (w/v) cell suspension in 10 mM Tris pH 7.5/2 mM CaCl2 with protease inhibitor (Roche, 11836170001), cleared with centrifugation at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed in assay buffer (1% NP-40) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche) ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted from adherent cells by scraping into extraction buffer (1× LysisM, 1× protease inhibitor cocktail, 2 x phosphatase inhibitor cocktail (all Roche), 2 mM sodium orthovanadate (Sigma ...
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bioRxiv - Microbiology 2022Quote: Cells were harvested and washed with ice-cold PBS and lysed in RIPA buffer and protease inhibitor (Roche, Cat.04693116001) for 30min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability analysis by lactate dehydrogenase (LDH) release assay was performed with the Cytotoxicity Detection Kit (LDH) (Roche, Mannheim, Germany) and has also been described 23,31.
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced in HEK293T cells using helper plasmids (VSVG and psPAX2) with X-tremeGENE 9 DNA Transfection Reagent (Roche). Target cell lines were infected with the virus and 10 μg/ml polybrene ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were plated in 6-well plates (100,000 cells/well) and transfected the next day using X-tremeGENE™ 9 DNA (Transfection Reagent, Roche), with HA-NLGN1 + AP-MDGA1 or AP-MDGA2 + BirAER (1 μg/well) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Labelled cells were washed twice with 1mL FACS buffer and resuspended in 500uL FACS buffer containing DNAse I (10ug/mL, Roche). Sorting of the cells was performed into 500uL RNAlater on FACSAria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with cold phosphate buffered saline (PBS) then scraped into cold PBS containing protease inhibitors (Roche, Laval, QC) and 5 mM sodium fluoride (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension by incubating the retinas in 1mL of HBSS containing 10U of papain (Roche) and 200µM cysteine ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral vector expressing the corresponding sgRNA was transfected into HEK293T cells with lentiviral packaging vectors VSV-G and Delta-VPR using XtremeGene transfection reagent (Roche). After 24 hr ...
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bioRxiv - Bioengineering 2021Quote: ... P14 CD8+ T cells were activated for 24 h as described above and resuspended in T cell media + 30 U/ml rhIL-2 (Roche) at 2 × 106 cell/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC lysis buffer (1.5 ml buffer per gram cell pellet) and the complete stock solution (1 tablet Complete EDTA-free (protease inhibitor cocktail, Roche) and 50 μl benzonase nuclease cell resuspension (PSF ...
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bioRxiv - Developmental Biology 2022Quote: RNA isolated from one E9.5 tailbud or 1mg RNA isolated from the in vitro cells was used as template for cDNA synthesis (Roche, 04897030001) using random hexamers and eluted in 200µl and 100µl H2O respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were centrifuged at 300 g for 5 minutes and red blood cells were lysed from samples using RBC lysis buffer (Roche) in 5 ml for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... All viruses were packaged for 48 hours following transfection of HEK-293T cells using X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Neuroscience 2019Quote: ... cells were washed with cold PBS and lysed on ice with RIPA buffer containing complete mini protease inhibitor cocktail (Roche), followed by bath ultrasonication in chill water and centrifugation for total protein extract ...
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bioRxiv - Cell Biology 2019Quote: Lysates from A375 cells stably expressing FLAG-EZH2 with or without Dox-inducible expression of FLAG-USP7 were prepared by incubating the cells in lysis buffer containing protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the candidates were PCR amplified from a U2OS (human bone osteosarcoma cell line) genome prep as template with the Kapa Hifi Hotstart Polymerase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were collected from the cell culture by centrifugation and homogenized in PBS buffer with cOmplete EDTA-free protease inhibitor (Roche). The cells were solubilized by supplementing dodecyl maltoside (DDM ...
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bioRxiv - Genetics 2019Quote: Protein was isolated from 10 brain-imaginal disc complexes were dissected from wandering L3 larvae or S2 cells and homogenized in 1X Laemmli/RIPA buffer containing 1X protease inhibitors (Roche cOmplete Mini EDTA-free protease inhibitor tablets ...
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bioRxiv - Neuroscience 2019Quote: Transfected neuroblastoma cell line SH-SY-5Y were resuspended in iced RIPA buffer added of protease inhibitors (#04 693 159 001 Roche) and subjected to sonication (Biorupture sonication system ...
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bioRxiv - Physiology 2020Quote: ... Patch-clamp experiments on HCN1 and HCN2 were performed with transient transfection of 2 μg of either pCDNA3.1-HCN1 or pCDNA3.1-HCN2 (generously provided by Dr. Eric Accili) in HEK293 cells using Fugene6 (Roche, Basal, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... memory B cells were plated at 6 B cells in 55 μl per well into 96 × 384-well plates in B cell media supplemented with 100 U ml−1 IL-2 (Roche), 50 ng ml−1 IL-21 (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were harvested in ice-cold PBS using a cell scraper and lysed by sonication for three times 10 seconds in ice-cold PBS containing Complete protease inhibitors (Roche). Unbroken cells were removed by centrifugation at 500 g for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the transfected cells were lysed in 800 ul PBS with 1% Triton and protease inhibitor (Roche cOmplete EDTA-free tablets), and cleared of debris by spinning in a table-top centrifuge (Eppendorf 5424R ...
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... 25μl of blood was mixed with 75μl of PBS to obtain cell counts on a hematological analyzer (Sysmex KX-21N, Roche Diagnostics). The remaining blood was red blood cell-depleted using hypotonic lysis buffer (150mM NH4Cl ...
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bioRxiv - Microbiology 2021Quote: ... release into growth medium (as direct indicator of cell membrane integrity loss) was performed with the Cytotoxicity Detection KitPLUS (Roche) according to the supplier’s manual.
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bioRxiv - Cancer Biology 2021Quote: Lysates were prepared from tumor tissue or cultured cells using RIPA buffer supplemented with fresh protease and phosphatase inhibitors (Roche). Protein quantification was performed using the BCA Protein Assay (G Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... 70-80% confluent CHO-K1 cells were transfected in 35 mm dishes using X-TREMEGENE 9 Transfection Reagent (Roche, 6365787001). Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... of 90-100% confluent HEK293T cells were lysed directly using CERI and NER buffer supplemented with 1x protease inhibitor cocktail (Roche), 1x PhosSTOP (Roche) ...
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bioRxiv - Genomics 2019Quote: ... the cells were transfected with 10 μg of the plasmid libraries (HSS, ORI, and pGL4) using X-tremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection of pTON-BIOPS-Flag-EGFP-FOXO1/FOXO3 and pCDNA-HA-E2F1 in HEK293T cells was performed using ExtremeGene 9 (Roche). Transfection of siRNA smartpools targeting EMI1 ...