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Citations for Roche :
2901 - 2950 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Pellet of frozen cells corresponding to 10E6 cells were thawed in ice and resuspended in 500 µL of Buffer A with protease inhibitors (Roche). 150 µL of suspension were supplemented with 180 µL of buffer B and sonicated 5 min at 4°C by BioRuptor (high power ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
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bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... transfected HEK293T cells were harvested at the indicated time points and resuspended in ice-cold RIPA buffer with protease inhibitor cocktail (Roche). Total protein was quantified by BCA assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: The retroviral recombination substrate pINV-12/23 was introduced into primary leukemic cells utilizing X-treme GENE HP DNA Transfection Reagent (Roche). Recombina-tion efficiency of pINV-12/23 was evaluated through flow cytometry analysis for mouse CD90 (mCD90 ...
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bioRxiv - Biochemistry 2024Quote: ... The pcDNA3 constructs were used for transient transfection in HEK293 cells using an X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
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bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
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bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral vectors expressing sgRNAs were transfected into HEK293T cells with lentiviral packaging vectors CMV VSV-G and psPAX2 using XtremeGene 9 transfection reagent (Roche). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: hiPSC-DANs cell pellets were collected in ice-cold PBS and were homogenised in RIPA buffer containing protease (cOmplete EDTA-free, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Pathology 2024Quote: Protein lysate samples were prepared from snap frozen aortas and cells in RIPA solution (Cat# P0013B; Beyotime) supplemented with protease inhibitor (Cat# 05892791001; Roche). Denatured protein lysates were resolved by 8% −10% (wt/vol ...
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bioRxiv - Cell Biology 2024Quote: ... cultured cells were washed with PBS and lysed in ice-cold RIPA buffer supplemented with EDTA-free cOMPLETE protease inhibitor (Roche) on ice ...
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bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from 200 µL of indicated cell supernatants using a High Pure Viral RNA Kit (Roche, Germany). The extracted RNA was eluted in 50 µL of elution buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and from WT and Hcar1 KO mice were lysed in RIPA buffer (cell signaling) supplemented with 0.1 mg/ml PMSF and proteinase inhibitor cocktail (11697498001, Roche, USA), and then were homogenized thoroughly (BRANSON Sonifier 150 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with cold PBS and lysed in IP lysis buffer supplemented with protease inhibitors (Roche, Basel, Switzerland, #05892791001). The supernatant was obtained by centrifugation at 10000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted, resuspended in HSB (PBS supplemented with 150 mM NaCl, 1 mM DTT, protease inhibitor cocktail Complete (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Cell Biology 2023Quote: Heart sections (described above) were used for TUNEL assays using the in situ cell death detection kit (Cat No. 11684817910, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Cell growth of shRNA-treated cell lines was assayed either through crystal violet staining or using the Promega CellTiter-Glo proliferation kit (Roche) or alternatively by live-cell confluence measurements with the IncuCyte (Essen BioScience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with 10 ml of cold 1x PBS supplemented with proteinase inhibitors and phosphatase inhibitors (Roche # 4693132001) and the pellets were snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were syringe lysed in lysis buffer (8M urea, 50mM EPPS pH 8.5, 150mM NaCl, and Roche protease inhibitor tablet) and the resulting lysates were cleared via centrifugation.
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bioRxiv - Microbiology 2023Quote: Cells transiently expressing M2-1-GFP or GFP as control were lysed with cell lysis buffer (CST) supplemented with complete mini-EDTA-free protease inhibitors (Roche) on ice and cell debris removed by centrifugation ...
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bioRxiv - Genomics 2023Quote: Chromatin samples prepared from 40 million ES or 501 Mel cells were supplemented with protease inhibitors (Complete Protease Inhibitor Cocktail, Roche) and loaded on a 9.9 ml manually poured 10-30 % sucrose gradient containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Cells and tissues were lysed in NP-40 lysis buffer or SDS lysis buffer supplemented with protease inhibitor (Roche, 04693159001) and then centrifuged at 4 °C to obtain cellular lysate ...
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bioRxiv - Neuroscience 2023Quote: ... 293 cells (ATCC, CRL-1573) and HEK 293T (ATCC, CRL-3216) were transiently transfected with cDNAs using Xtreme Gene HP (Roche). For ICC ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended and homogenized in buffer B supplemented with 2 mM MgCl2 and 1x protease inhibitor (Roche). The protein was extracted by incubating with 1% GDN (Anatrace ...
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bioRxiv - Developmental Biology 2023Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RPE-1 cells were trans-fected with the different Flag constructs for 48 h using X-tremeGENE 9 DNA reagent (Roche). Cells were lysed in 25mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml hypotonic buffer supplemented with 10 µg/ml PMSF and protease inhibitor cocktail (Roche). Cells were incubated on ice for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were blocked and permeabilized overnight (STORM) or for 2h (STED and LSCM) (1% Roche Western Blotting Reagent (WBR), Merck ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
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bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
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bioRxiv - Cell Biology 2023Quote: Apoptotic cells were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Developmental Biology 2023Quote: The distribution of apoptotic cells was determined by TdT-mediated dUTP nick-end labeling of the fragmented DNA (TUNEL, Roche). Briefly ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed in Homogenization buffer (20 mM Tris, pH 7.4, 10mM EDTA, supplemented with phosphatase inhibitors (Phosphostop, Roche, Germany), and protease inhibitors (cOmplete ...
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bioRxiv - Immunology 2023Quote: ... Stored synovial tissue samples were then thawed and disaggregated into single-cell suspensions by mincing and digesting with 100 µg/mL LiberaseTL (Roche) and 100 µg/mL DNaseI (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... the cells washed twice with cold PBS and then scraped from the cell culture dishes in 1 mL PBS with cOmplete protease inhibitor by Roche. The cells were washed once with lysis buffer 1 (140 mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded at 400,000 cells per well in 6-well plates and transfected the day after with indicated cDNA constructs with FuGENE6 (Roche). Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining on 60 dpf paraffin embedded testes sections were performed using a fluorescein In Situ Cell Death Detection Kit (11684795910, Roche). Immunolabelling with Vasa antibody was done before the TUNEL staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were lysed on ice with a cell scraper and radioimmunoprecipitation assay lysis buffer (Pierce) supplemented with protease inhibitor tablets (Roche) and a phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...