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Citations for Roche :
2951 - 3000 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Cells were plated in 96-well plates and treated as indicated for 72 hours before performing MTT survival assay using Cell Proliferation Kit 1 MTT (Roche) following the manufacturer’s instructions ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... cells and mice tissue were collected and fragmented in lysis buffer (Beyotime, Shanghai, China) supplemented with protease inhibitors cocktail (Roche). Isolated proteins were then segregated by gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... Neuro2A and H4 cells were transfected 24 to 48 h before observation with calcium phosphate or with X-tremeGENE9 (Roche), following the manufacturer’s instructions ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: For sample preparation, cells were lysed in RIPA lysis buffer strong (Beyotime, P0013B) supplemented with protease inhibitor cocktail (Roche, 4693116001). Cells were lysed at 4 °C for 20 min and spun at 14000 rpm for 10 min to remove insoluble debris ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was caried out using the In Situ Cell Death Detection TMR rot kit (Roche, cat. no. 12156792910) according to the manufacturers protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The daily sampling procedure consisted of cell density and viability using a Cedex HiRes analyzer (Roche Diagnostics, Indianapolis, IN, USA), metabolites (lactate ...
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bioRxiv - Molecular Biology 2023Quote: ... The frozen cell pellets were resuspended in lysis buffer (50 mM TRIS pH 7, 150 mM NaCl, 1 mM EDTA, Roche’s complete Protease inhibitors ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed for 30 min on ice in lysis buffer supplemented with fresh 1 x protease inhibitor cocktail (Roche). Unless otherwise indicated ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with PBS for three times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche) on ice for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... The treated lymphoma cells were cooled down by addition of the three-fold volume of ice-cold DPBS supplemented with 1xPhosSTOP (Roche) and 100µM sodium orthovanadate ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:10 in water and qPCR was performed with 3 μL of each diluted cell lysate using the LightCycler 480 Probes Master mix (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Lysates from crosslinked cells were prepared by adding 1ml of 1x PXL lysis buffer + quenchers + protease inhibitors (Roche, cat. #11873580001) and triturating to disrupt cells ...
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bioRxiv - Cell Biology 2023Quote: Wild-type HMC3 cells and potential G6PD deficient colonies were used to make cells lysate using RIPA buffer supplemented with 1x Protease inhibitor cocktail (Roche). Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma) ...
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bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 ng of pCAG-mCherry were transfected into 80% confluent HEK293T cells in 24-well plates using FuGene6 (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T and HT1080 cells were incubated for 24 hours before the addition of 400 µg/mL and 100 µg/mL hygromycin (Roche) for selection ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed 80 h post seeding in RIPA buffer (Thermo, 89901) containing cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysate were centrifuged at 15000 rpm for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were washed once then resuspended in a 1:1 ratio with CHAPS lysis buffer + protease inhibitors (Roche Complete) and frozen dropwise in liquid nitrogen before freezer-mill lysis ...
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bioRxiv - Biophysics 2024Quote: ... 2.0 × 106 cells/mL of WT Neuro2a or OptoTau KI cells were incubated in 10 mM of HEPES buffer supplemented with 1% protease inhibitor (cOmplete, Roche), 1 µM of okadaic acid (Cayman Chemical) ...
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bioRxiv - Developmental Biology 2024Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche), commonly ...
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial fraction along with total cell lysate and cytosolic fractions were lysed in RIPA buffer that contained Complete mini protease inhibitor cocktail (Roche) and phosphatase inhibitor (PhosSTOP ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from dCasRx+gRNA transfected HEK293T cells was reverse transcribed using oligo dT and the Transcriptor First Strand cDNA Synthesis Kit (#04897030001, Roche) as manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Stable cell lines were established by selection with Schneider’s Drosophila medium supplemented with 300µg/ml hygromycin (Roche, Palo Alto, CA).
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bioRxiv - Cell Biology 2024Quote: Apoptosis was measured by Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling (TUNEL) assay using In Situ Cell Death Detection kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the three different cell types were assayed for telomerase activity according to the TeloTAGGG Telomerase PCR ELISA Kit (11854666910; Roche).
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bioRxiv - Immunology 2024Quote: ... Thawed synovial tissues were disaggregated into single-cell suspensions by mincing and digesting with 100 micrograms per milliliter LiberaseTL (Roche) and 100 micrograms per milliliter DNaseI (Roche ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The wells containing fixed cells were washed once with 1X PBS and stained with DAPI (50 μg/ml) for (Roche, 10236276001) 10 min at RT and protected from light ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Molecular Biology 2020Quote: ... washed with ice-cold PBS and re-suspended with 500µL of ‘IP-MS Cell Lysis Buffer’ which was supplemented with 1x cOmplete™ Protease Inhibitor Cocktail (Roche, 11697498001) and 1x PhosSTOP™ (Roche ...
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bioRxiv - Immunology 2019Quote: ... 300 ng of total RNA from three biological replicates per B cell subset was used as input for the KAPA stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems). Libraries were assessed for quality on a bioanalyzer ...
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bioRxiv - Microbiology 2019Quote: ... Twenty-four hours post-transfection cells were rinsed with PBS and lysed in NP40 buffer supplemented with cOmplete™ protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation and incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was added to a 50 µL aliquot of cells in addition to 50 µg boiled fish sperm carrier DNA (Roche 11467140001). Cells were equilibrated at 30° for 10 minutes after which 0.5 mL 40% Polyethylene glycol (PEG4000 ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Genetics 2019Quote: ... was used as a hypotonic solution and cells were dropped onto a slide with 1% PFA containing protease inhibitors (Roche 1836153). After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were washed twice in cold PBS and total RNA was extracted using a High Pure RNA isolation kit (Roche 11828665001). Reverse transcribed RNA was used for sequencing by the University of Lausanne ...
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bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed according to the manufacturer’s instructions (In Situ Cell Death Detection Kit, Fluorescein, Roche, #11 684 795 910). In brief ...
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bioRxiv - Neuroscience 2021Quote: Cytotoxicity in the cell cultures (primary cortical neurons and cell lines) was assessed using the cytotoxicity lactate dehydrogenase (LDH) detection kit according to the manufacturer’s instructions (Roche, Basel, Switzerland). Furthermore ...
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bioRxiv - Biochemistry 2022Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were prepared using 100-500 ng of cell free DNA (cfDNA) or sonicated genomic DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested from frozen plates by scraping into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001 ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Cancer Biology 2020Quote: Real-time measurements of cell migration and invasion were performed using the CIM-Plate 16 module of an xCELLigence System RTCA DP real-time cell analyzer (Roche, UK). For invasion measurement ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... assay [31] was performed on cryosections of retinal explants using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... were added and after an additional 6 hours BrdU was added and a cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences).
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bioRxiv - Cancer Biology 2020Quote: Neurosphere cell cultures and PC3 were synchronized at metaphase by treating with 80 ng/ml Colcemid (Roche, 10-295-892-001) overnight ...