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Citations for Roche :
2851 - 2900 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... cells were collected in lysis buffer (TBS + 1% Triton X-100) containing protease inhibitors (Complete Mini EDTA-Free, Roche 04693159001), briefly sonicated ...
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bioRxiv - Immunology 2020Quote: ... supplemented with BaseMuncher nuclease (Expedion; 1 ml per 40 ml cell suspension) and Complete EDTA-free protease inhibitor mix (Roche). The extract was precleared by centrifugation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were syringe lysed in lysis buffer (8M urea, 50mM EPPS pH 8.5, 150mM NaCl, and Roche protease inhibitor tablet) and the resulting lysates were cleared via centrifugation.
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bioRxiv - Physiology 2019Quote: ... TUNEL staining was conducted following manufacturer’s protocol (In Situ Cell Death Detection Kit, Version 17, Roche Diagnistics GmbH, Mannheim, Germany). Briefly ...
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bioRxiv - Cell Biology 2019Quote: Lentivirus was produced using 293T cells (a gift from G. Green, University of Chicago, Chicago, IL) using Fugene 6 transfection reagent (Roche) to transfect the lentiviral vectors ...
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bioRxiv - Genetics 2021Quote: Overexpression plasmids were respectively transfected into U251-APP cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). Cells grown in 6-well plate were transfected by overexpression plasmid of the target gene or its corresponding control plasmid (each 2 μg ...
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bioRxiv - Biochemistry 2021Quote: For transient transfection cells were seeded and after 18 h cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche), according to the manufacturer’s protocol (for 12-well plate format ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Sample quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells at a confluence of 20-30% were transfected with the respective EGFP-C3 constructs using FuGENE6 transfection reagent (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Metabolic activity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-assay (Cell Proliferation Kit I, Roche Germany, Mannheim) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested by centrifugation and lysed in 1% Nonidet P40/1 mM EDTA/PBS containing Complete protease inhibitors (Roche), and nuclei were removed by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... sgRNA expressing vector along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK-293T cells using the XTremeGene 9 transfection reagent (Roche). Similarly ...
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bioRxiv - Cancer Biology 2021Quote: ... Native fluorescence in situ hybridization (FISH) was performed by incubating cells with a hybridization mix (70% formamide, 1mg/mL Roche FISH blocking reagent 11096176001 ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Genomics 2022Quote: ... The reaction was quenched using 125 mM glycine for 5 minutes with rotation and the cells were washed three times using ice-cold PBS containing complete protease inhibitor cocktail tablets (Roche). Cells were resuspended in lysis buffer (1% SDS ...
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bioRxiv - Biochemistry 2021Quote: The confluent cells were washed with PBS and lysed with ice-cold NP-40 Lysis Buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Naïve and activated B cells were incubated on ice for 20 min in NP-40 lysis buffer in the presence of protease inhibitors (Roche) and lysates were cleared by centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
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bioRxiv - Microbiology 2021Quote: Fifteen nanograms of whole-cell DNA was analyzed by qPCR using 300 nM primers and SYBR green master mix (Roche). The reaction conditions consisted of a 15-min 95°C activation cycle ...
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bioRxiv - Cell Biology 2020Quote: ... Whole cell lysates were obtained by directly exposing cells to sodium dodecyl sulphate (SDS) lysis buffer supplemented with phosphatase and protease inhibitors (Roche). Membranes were incubated in Roti block (Roth ...
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bioRxiv - Immunology 2021Quote: Fresh cell samples were lysed in RIPA buffer (Thermo) supplemented with complete EDTA-free Protease inhibitor cocktail and phosphatase inhibitors (Roche) and sonicated on a Bioruptor (Diagenode) ...
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bioRxiv - Immunology 2020Quote: ... TUNEL assays were performed on sectioned tissue using the TMR red In Situ Cell Death Detection Kit (Roche, Basel, Switzerland), following the manufacturer protocol for labeling of cryopreserved tissues ...
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bioRxiv - Immunology 2020Quote: ... SHIV-Ag85B was prepared by transfecting pSHIV-Ag85B into 293T cells using FuGENE 6 Transfection Reagent (Roche Diagnostics, Indianapolis, IN), and the culture supernatant was stored at 48 h after transfection in liquid nitrogen until use.
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bioRxiv - Immunology 2021Quote: BMDMs and THP-1 macrophage-like cells were lysed in ice-cold RIPA buffer supplemented with complete protease inhibitor and phosphatase inhibitor cocktails (Roche). Protein concentration was measured by the Pierce™ BCA Protein Assay Kit (Thermo Scientific-23227) ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells and tissue were lysed with RIPA buffer (Beyotime Biotechnology) supplemented with PMSF (Beyotime Biotechnology) and protease inhibitor cocktail (Roche). Mettl3 (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... The lymphocytes from the immunized mice were fused with myeloma P3U1 cells at a ratio of 3:1 by mixing in 50% polyethylene glycol (Roche). The fused cells were dispersed in 80 ml of GIT medium (Wako ...
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bioRxiv - Molecular Biology 2021Quote: A-431 cells were transiently hNAA40-flag transfected for 24 h with hNAA40-flag expression vector and using Fugene HD (Roche) according to the manufacturer’s instructions and as described above ...
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bioRxiv - Developmental Biology 2022Quote: Cells in culture wells were washed with PBS before harvesting with TriPure TRIzol reagent (Cat# 11 667 165 001, Roche). The cell-TRIzol mixtures were transferred to collection tubes and homogenized with Tissuelyser II (Qiagen) ...
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bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... The inner bottom surface of the flow cell is coated with an hydrophobic solution (nitrocellulose or Sigmacote) to allow attachement of anti-Digoxigenin (11093274910, Roche) Lionnet et al ...
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bioRxiv - Cell Biology 2022Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: The activity of telomerase in the cell lines was detected using the TeloTAGGG telomerase PCR ELISA Kit (Roche, Cat# 12013789001). The assay was performed according to the manufacturer’s instructions and repeated in triplicate ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... HEK 293 cells stably-expressing GFP-DFCP1 and RFP-ATG9 were generated by transfection of HEK 293 GFP-DFCP1 cell with RFP-ATG9 using X-tremeGENE 9 (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Whole endothelial extracts were prepared from 70-80% confluent cells in lysis buffer (3% SDS, 60 mM sucrose, 65 mM Tris (pH 6.8) containing protease inhibitor cocktail (Roche-11836170001). Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™ ...
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bioRxiv - Cancer Biology 2022Quote: A vector control plasmid and WT and mutant Plk3 expression plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Roche) as described previously (Li et al. ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
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bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... washing them twice with PBS containing 1 mM CaCl2 and MgCl2 and collecting cells by scraping them in CaCl2/MgCl2 PBS containing cOmplete protease inhibitor cocktail (Roche). Lysates were cleared by centrifuging at 1000 x g for 10 min at 4 °C and pellets were resuspended in PBS with 1 % Igepal ...
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bioRxiv - Microbiology 2022Quote: ... 33.75 µL cells were mixed with a PCR master mix containing 37.5 µL KAPA HiFi HotStart ReadyMix (Roche, CN KK2602), 2.25 µL second strand synthesis primer SMRT_dT (10 µM ...
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bioRxiv - Microbiology 2022Quote: ... A cell pellet was resuspended in 250 μL Tris-EDTA buffer and 50 μL 30 g L-1 lysozyme (Roche) in Tris-EDTA buffer ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... the cells or tissues were extracted on ice using standard CHAPS buffer (60 μL per 106 cells) supplemented with a cocktail of protease inhibitors (Roche) and 20 units of RNase inhibitor (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 8-keto-NeuAc derivatives for 24 h and proliferation measured using WST-1 (Roche Applied Science) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Molecular Biology 2022Quote: Pellet of frozen cells corresponding to 10E6 cells were thawed in ice and resuspended in 500 µL of Buffer A with protease inhibitors (Roche). 150 µL of suspension were supplemented with 180 µL of buffer B and sonicated 5 min at 4°C by BioRuptor (high power ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...