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Citations for Roche :
2651 - 2700 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... These were identified by immunohistochemical staining (IHC) with antibodies against T cells: CD3 (Roche Cat # 790-4341, pre-diluted), CD4 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: (Thawed) Cell pellets were lysed on ice for at least 10 min with 1x protease inhibitors cocktail (PIC; Roche) and 1x quenchers (10 mM NaN3 ...
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bioRxiv - Genomics 2021Quote: ... followed by lysing the cells on ice for 3 min in 50 μl of ATAC-seq RSB containing 0.1% NP40 (Roche), 0.1% Tween-20 (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... Glioma graft cells were labeled by the anti-human Vimentin antibody (mouse monoclonal, V9, 790-2917, Roche, pre-diluted), followed by a mouse secondary antibody (rabbit polyclonal anti-mouse biotin antibody ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Microbiology 2022Quote: ... 2013)) were transfected in HeLa cells 24 h before infection using X-tremeGENETM HP DNA Transfection Reagent (Roche, 6366244001), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested and re-suspended in buffer A supplemented with 10 μM ZnCl2 and protease inhibitor (Complete, Roche). Cells were then homogenized by sonication and the crude extract was centrifuged for 30 min at 18,000□×□g ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: Cells were lysed using RIPA lysis and extraction buffer with protease inhibitors and phosphatase inhibitors from Roche (Indianapolis, IN). Protein concentration were determined with the Bio-Rad protein assay (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed twice with ice cold PBS and scraped into cold PBS with phosphatase inhibitors (PhosSTOP, Roche). Pelleted cells were snap-frozen and lysed with 8M urea buffer with 100nM TEAB (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed by passive lysis buffer (25mM Tris-HCl, 150mM NaCl, 0.5% CA630) containing protease inhibitor cocktail (Roche). The cells were lysed in SDS loading buffer and boiled for 5-10min ...
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bioRxiv - Genomics 2022Quote: ... Trim24 knockout TC-1 ES cells (background 129S6/SvEvTac) carrying an RMCE selection cassette (described previously82) were selected under hygromycin (250μg/ml, Roche) for 10 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... ∼600 µL of 0.5 mm diameter glass beads were added and cells were lysed by bead beating on a MagNA Lyser (Roche) three times for 45 s each at 4500 RPM with 2 min rests at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Physiology 2022Quote: ... fixed and proceeded with colorimetric immunoassay for the quantification of cell proliferation according to manufacturer instructions (Roche, Germany, 11647229001).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were subsequently washed with phosphate-buffered saline (PBS) and re-suspended in PBS containing protease inhibitors (Roche, Canada). Cells were heated at 54°C for 3 min in a thermal cycler (VeritiPro ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions of two lung samples from mice of the same sex were pooled and washed with a 2X volume of ACK Lysing Buffer (Quality Biological) as above to lyse red blood cells and then were resuspended in PBS with 0.5% BSA (Roche) and 2 mM EDTA (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Cultured cells were first washed twice with sterile 1X PBS and subsequently scraped from the wells using a cell scratcher in 200µl lysis buffer (RIPA buffer supplemented with 1x Roche complete mini protease inhibitor cocktail and Phospho-Stop phosphatase inhibitor) ...
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bioRxiv - Physiology 2024Quote: ... RNA oligonucleotides were delivered to MLO-Y4 cells using the X-treme Gene siRNA Transfection Reagent (Roche; Cat#04476093001) according to a protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... MIO-M1 cells were transfected with 20 µg/ml of pEGFP or pEGFP-SIK2 vector using FugeneHD (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl+ protease inhibitors (Roche cat # 4693116001) and quantified by BCA assay (Pierce cat # 23223) ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Genetics 2024Quote: ... The testis cells were immediately dissociated through homogenization in 1X PBS containing proteinase inhibitor cocktail (PIC, cOmplete tablets Roche) (PBS+PIC) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with indicated concentrations of SN-38 or DMSO for 72 h before MTT assay (Roche, 11465007001), according to the manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured for the indicated amount of time with or without human IL-2 (TECINTM; teceleukin, ROCHE), human IL-15 (247-IL/CF ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated from cells and reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Gene expression was quantified using FastStart Roche SybrGreen (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell volume was determined again and 2 volumes cold buffer A++ (Buffer A + cOmplete protease inhibitors (Roche, cat 04693159001) and 0.15% NP40) ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and lysed with 1x RIPA buffer (CST, #9806) supplemented with 1x protease inhibitor cocktail (Roche, #693116001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Neuroscience 2022Quote: Cells were collected by first washing them with ice-cold 1x PBS supplemented with 0.5x protease inhibitor cocktail (Roche) and then collecting them with a cell scraper in PBS/0.5x protease inhibitor in an eppendorf tube ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometry analysis was performed on cell suspensions from kidney homogenates that were digested with 0.2 mg/ml liberase (Roche) and 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 cells were transfected with FAM-labelled hairpins using X-tremeGENE 9 DNA Transfection Reagent (Roche, USA; 1.0 µL). After 16 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were lysed using 200 μL/well cell lysis buffer (100 mM Tris-HCl pH 8.8, 1 % SDS) and protease inhibitors (cOmplete, Roche) on ice for one h ...