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Citations for Roche :
2951 - 3000 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Reverse transcription and PCR were performed on 2µg of purified RNA using the Transcriptor Reverse Transcriptase (Roche). RNA was first subjected to DNAse (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Single oocytes in PCR tubes were thawed and incubated with 0.2 mg/mL of DNase I (Roche) at 37°C for 30 min on a shaker ...
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bioRxiv - Genomics 2022Quote: ... Input and immunoprecipitated DNA amounts were quantified by real-time PCR with a LightCycler 480 system (Roche) using SYBR Green incorporation according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: All PCR amplifications for Nanopore library preparations were done with Kapa Hifi Hotstart polymerase (Roche Sequencing Solutions): initial denaturation ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed in 25 µl reaction mix containing 2x KAPA HIFI HotStart Ready-mix (Roche #07958935001), 10µM primers and 10 ng of gDNA template from stool or tissue under the following conditions ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time PCR reactions were performed in 96-well plates on a Light Cycler II 480 (Roche) device ...
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bioRxiv - Plant Biology 2019Quote: ... instrument and the DNA quantified by PCR on a LightCycler 480 II (Roche Molecular Systems, Pleasanton, CA) using the Library Quantification Kit (Roche Molecular Systems ...
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bioRxiv - Genomics 2019Quote: ... We then added 15 µl PCR master mix containing 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems)) and 0.5 µl 10 µM PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT) ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR (qPCR) reactions were prepared with LightCycler 480 DNA SYBR Green | Master reaction Mix (Roche Diagnostics). Primers are used at 0.2 μM and presented in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2019Quote: Quantitative reverse transcription PCR was performed with a LightCycler R® 480 II System (Roche Diagnostics, Switzerland). The PCR reaction mixture (10 μl total volume ...
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bioRxiv - Microbiology 2019Quote: PCR results and bio-clinical data were exported from LightCycler® 480 release 1.5.0 software (Roche®) and DxLab® software (MedaSys® ...
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bioRxiv - Molecular Biology 2019Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using the KAPA HiFi HotStart ReadyMix (Kapa Biosystems). i5 and i7 multiplexing barcodes were added in a second round of PCR and final gel-purified products were sequenced on Illumina NextSeq500 or HiSeq2500 systems to determine sgRNA representation in each sample ...
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bioRxiv - Genomics 2019Quote: K562 cDNA was used to amplify region of interest by PCR using FastStart Taq DNA polymerase (Roche) and gene specific primers (Supplementary Table 5) ...
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bioRxiv - Genomics 2020Quote: Northern probes were first synthesized using the PCR DIG Probe Synthesis Kit (Roche # 11 636 090 910). The 512 bp frq probe was amplified from wild-type Neurospora genomic DNA with primers ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed in 25 μl reactions containing 12.5 μl KAPA HiFi Hotstart ReadyMix [2X] (Kapa biosystems), 1.25 μl 5 μM mPing fusion primer (Supplemental Table 3) ...
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bioRxiv - Biochemistry 2020Quote: All libraries were constructed by consecutive extension PCR amplification by using KAPA HiFi HotStart ReadyMix (Roche, Switzerland) and NNK randomized primers (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The qPCR reactions were performed with a Light Cycler 480 Real-Time PCR machine (Roche Applied Science) with the following run protocol ...
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bioRxiv - Neuroscience 2020Quote: ... For further cDNA amplification 20 µl of PCR mix [1xKAPA HiFi Hotstart ready mix (Roche, Basel, Switzerland), 0.5 µM C1-P1-PCR-2 (5’-GAA TGA TAC GGC GAC CAC CGA T -3’) ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR reactions were carried out in triplicate with SYBR Green I Master Mix (Roche S-7563) on a LightCycler 480 system (Roche) ...
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bioRxiv - Neuroscience 2020Quote: DNA was extracted from mouse ear biopsies using High Pure PCR Template Preparation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was amplified by real-time PCR with the LightCycler 480 SYBR Green I Master mix (Roche). Qiagen QuantiTect primers were used for detection of UCP1 (QT00097300) ...
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bioRxiv - Biochemistry 2021Quote: ... The plate was sealed and heated in a real-time PCR system (Light Cycler 480, Roche diagnostic) from 20°C to 80°C in increments of 0.5°C/minute ...
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bioRxiv - Microbiology 2020Quote: ... followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche) and the LightCycler® Nano (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with a LightCycler® 480 SYBR Green I Master Mix kit (Roche). The primers used in the indicated gene array are listed in Table S1 ...
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bioRxiv - Plant Biology 2020Quote: Quantitative PCR (qPCR) was performed in triplicate using a 96-well plate-based (Roche Diagnostics, Penzberg, Germany) in a Thermal Cycling StepOnePlus (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR analysis was carried out on a LightCycler® 480 instrument (Hoffmann-La Roche, BSL, Switzerland) using the SYBR Green detection system ...
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bioRxiv - Systems Biology 2020Quote: ... The cloning of the gRNAs sequences was performed via full plasmid PCR amplification with the KAPA (Roche) polymerase using 60-mer oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Plant Biology 2021Quote: ... The reaction included SYBR green PCR master mix (light Cycler 480 SYBR Green I Master, Roche Diagnostics) with 4-folds diluted cDNA in the total volume of 20μl (Pal et al. ...
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bioRxiv - Neuroscience 2020Quote: ... SYBR green quantitative PCR was performed using the LightCycler® 480 system (Software 3.5, Roche Molecular Biochemicals). To determine the relative enrichment ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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bioRxiv - Microbiology 2020Quote: ... The first round of PCR amplified the V3-V4 region using HIFI HotStart Ready Mix (Kapa Biosystems). The first round of PCR used a denaturation temperature of 95 °C for 3 min ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of the cDNA was used to perform the real-time PCR using Sybr green reagent (Roche). The primers for real-time PCR in HeLa and HAP1 cells are described in [42] ...
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bioRxiv - Genomics 2020Quote: ... DNA was amplified by 9 cycles of PCR using the Kapa Hifi Uracil + DNA polymerase (KAPA Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR was performed using TB green Premix Taq on a LightCycler 480 Instrument II (Roche). Primers are listed in the Supplemental Materials ...
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bioRxiv - Cell Biology 2021Quote: ... on the Real-Time PCR Systems (CFX96, Bio-Rad, USA; or LightCycler 96, Roche Life Science, Swiss). The primers used in this study are listed in Table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... Purified DNA was quantified by real-time PCR with KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) and gene-specific primers using the CFX Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was carried out using the Light-Cycler 480 SYBR Green I Master (Roche). Each 20 μl reaction contained 10 μl of 2x SYBER Green I Master mix ...
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bioRxiv - Biochemistry 2022Quote: ... Each PCR product was quantified by qPCR using the Kapa Sybr Fast qPCR Master Mix (Kapa Biosystems) on a Bio-Rad CFX instrument ...
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bioRxiv - Genetics 2022Quote: The qRT-PCR experiments were conducted using LightCycler® FastStart DNA Master PLUS SYBR Green I (Roche) for TAHRE experiments and Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific™ ...
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bioRxiv - Cancer Biology 2022Quote: ... The qRT-PCR reactions were done using the LightCycler 480 SYBR green I kit (Roche Cat #04077516001), using the following primers at a concentration of 5 μM ...
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bioRxiv - Microbiology 2019Quote: Real-time PCR amplifications were carried out using LightCycler® 480 Probes Master kit (Roche diagnostics, France) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... followed by real time quantitative PCR using Kapa SYBR Fast qPCR kit (Kapa Biosystems, Catalog No. KR0389_S) and Light Cycler 96 (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from 2.5×107 cells using the High Pure PCR Template Preparation kit (Roche). 1.1 µg DNA was then either treated with 10 U recombinant E ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR experiments were performed using iTaq Universal SYBR Green Supermix and a Light Cycler 480 (Roche). Each sample was analyzed in triplicate and normalized to Rap2l transcript abundance ...
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bioRxiv - Microbiology 2020Quote: ... Cobas SARS-CoV-2 PCR Test for the Cobas 6800 System (Roche Molecular Systems, Branchburg, NJ, USA) was used to detect the presence of SARS-CoV-2 [11].
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bioRxiv - Cancer Biology 2020Quote: ... PCR mix was dispensed in a volume of 50 μl using 1x Hifi HotStart Readymix (Kapa Biosystems) and 0.8 mM Template-Switch-PCR primer ...
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bioRxiv - Biochemistry 2021Quote: ... Purified DNA was quantified by real time PCR with KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) and gene specific primers using a CFX Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mix consisted of 5 μL 2X LightCycler® 480 Probes Master (Roche, Cat. No. 04707494001), 1 μL MDA product ...
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bioRxiv - Genetics 2022Quote: ... The PCR procedure was performed by adding 25μL 2 × KAPA HiFi HotStart Ready Mix (KAPA biosystems, KM2602) with 2.5ul primers of NEB Oligos kit and 2.5ul Illumina universal primers ...
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bioRxiv - Genetics 2022Quote: ... Analysis of gene expression was performed using a The LightCycler® 480 real-time PCR System (Roche). The gene ACT1 was used as endogenous control ...