Labshake search
Citations for Roche :
2901 - 2950 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisbioRxiv - Molecular Biology 2024Quote: ... Real-time Q-PCR thermal cycling reactions were performed by the LightCycler 480 (Roche, Meylan France) directed by the LightCycler 480 Software (version 1.5.1.62) ...
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bioRxiv - Genomics 2024Quote: ... treated with USER enzyme and amplified by PCR using HiFi HotStart Uracil+ Ready Mix (Kapa/Roche). Completed libraries were quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems ...
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bioRxiv - Genomics 2024Quote: ... pegRNAs were amplified from the oligo pool via PCR using KAPA HiFi HotStart ReadyMix (#KK2602, Roche). pegRNA oligo subsets were specifically amplified from the oligo pool using primers specific for the library BC (Supplementary Table 9) ...
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bioRxiv - Microbiology 2024Quote: ... NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems), according to the QuikChange site-directed mutagenesis manual ...
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bioRxiv - Microbiology 2023Quote: ... Concentration of the pool was measured with quantitative PCR (KAPA Library Quantification Kit, Roche, Basel, Switzerland). Amplicon libraries were mixed with 5% PhiX and sequenced with MiSeq reagent kits v2 500 cycles (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The individual libraries were quantified using the KAPA real-time PCR system (ROCHE DIAGNOSTICS Cat # 07960441001), following the manufacturer’s instructions and using a custom-made series of standards obtained from serial dilutions of the PhiX DNA (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche) with the following cycle conditions ...
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bioRxiv - Immunology 2023Quote: ... ‘reverse’ 5’-GGGTACTTGATTTCATAGACTTTA-3’) were used in a qRT-PCR on a LightCycler 480 II (Roche). The data were analysed with LightCycler® 96 SW 1.1 (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The ERIC-PCR was performed with 25-μL volumes containing Taq DNA polymerase buffer 1X (Roche), 200 mM dNTP (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The qRT-PCR was performed using KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems) with StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Pathology 2023Quote: ... 2 canine gastrointestinal biopsies and 5 canine normal tissues (High Pure PCR Template Preparation Kit, Roche Applied Science ...
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bioRxiv - Microbiology 2023Quote: ... Quantifications were performed by real-time PCR on a Light Cycler instrument (Roche Diagnostics, Meylan, France) using the second-derivative-maximum method provided by the Light Cycler quantification software (version 3.5 (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μl reactions were prepared for each sublibrary with the KAPA HiFi PCR Kit (Roche, KK2502) containing 0.5 μl of the oligo pool resuspended to 20 ng/μl ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μl reactions were prepared for each sublibrary with the KAPA HiFi PCR Kit (Roche, KK2502) containing 0.5 μl of the oligo pool resuspended to 20 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... The library was amplified through nine PCR cycles using the Kapa HiFi HotStart Ready Mix (Roche) to generate sufficient material for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time quantitative PCR performed in duplicates in 384-well plates with a LightCycler 480 (Roche) using SYBR Green I Master (Roche ...
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bioRxiv - Immunology 2023Quote: ... and fractions 7–10 (cytosolic ribosomes) were pooled and treated with PCR grade proteinase K (Roche) in 1% SDS to release ribosome protected fragments ...
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bioRxiv - Genomics 2023Quote: ... and sequencing libraries were prepared from tagmented samples by PCR amplification using Kapa HiFi polymerase (Roche). For H3K4me3 ChIP ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative real-time PCR (qPCR) analysis was performed using a SYBR Green Mix qPCR kit (Roche) and the LightCycler 480 Detection System (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA levels were determined by quantitative real-time PCR using the Universal ProbeLibrary (Roche, Life Science) and LightCycler 480 Probes Master (Roche ...
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bioRxiv - Cell Biology 2023Quote: Polymerase chain reaction: 240 μL of the PCR supermix (1.66x KAPA HiFi HotStart readymix (Roche, 07958935001), 1.33 μM PCR primer ...
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bioRxiv - Plant Biology 2023Quote: ... The purified PCR products were prepared for the libraries using the Kapa DNA Hyper Kit (Roche) utilizing indexes from TruSeq DNA UD indexes for Illumina (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... The modified DNA was amplified with 13 cycles of PCR using Kapa U+ polymerase (Roche 07959052001) and indexed primers (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The first step PCR was performed in 25 µL reactions including KAPA HiFi HotStart ReadyMix (Roche), 515F50 and 806R51 primers (covering a ∼350-bp fragment of the 16S V4 region ...
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bioRxiv - Immunology 2023Quote: ... prior to gene expression analysis by real-time quantitative PCR (LightCycler, Roche; or QuantStudio 3, ThermoFisher). Quantitect SYBR green PCR mastermix (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR reaction was performed in duplicate using a LightCycler® 480 instrument II (Roche). Data are normalized relative to B2m (2(−ΔCt)).
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were quantified by real-time PCR using KAPA Library Quantification Kit for NGS (Roche) and assessed for size distribution on a Fragment Analyzer (Agilent ...
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bioRxiv - Biophysics 2023Quote: Differential scanning fluorimetry was performed using a Light Cycler 480 II Real-time PCR System (Roche). NP (in 50 mM Tris at pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... full-length KILR was amplified from T47D cDNA using the KAPA HiFi PCR Kit (Kapa Biosystems) and KCTD1-5 cDNA was synthesized by IDT ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was then performed using Taqman Fast Advanced MasterMix with Universal Probe Library Probes (Roche) with specific primers designed using the Roche Lifescience Assay Design Center (https://lifescience.roche.com/en_it/brands/universal-probe-library.html#assay-design-center ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNA was purified using the High Pure PCR Product Purification Kit (Roche Applied Science) [44].
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bioRxiv - Pathology 2023Quote: ... Viral titres were quantified by Real-Time PCR using the LightCycler480 SYBR Green I Master (Roche) and primers targeting eGFP (5’- GACGACGGCAACTACAAGA-3’ ...
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bioRxiv - Genomics 2023Quote: ... All qPCRs were carried out on a LightCycler 480 Real-Time PCR system (Roche Life Science). Oligonucleotide PCR primers ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche, Branford, CT, USA) with PCR amplification conditions optimised for each plant tissue (Table S2) ...
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bioRxiv - Genetics 2024Quote: ... PCR pre-amplification was performed by adding 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM ISPCR primers (5’-AAGCAGTGGTATCAACGCAGAGT ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPATM SYBR FAST qPCR Kit (KAPA Biosystems) and StepOnePlusTM Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... Quantivative reverse transcription PCR (qPCR) reaction was performed using KAPA SYBR FAST qPCR Master Mix (Roche) and gene-specific primers (Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole transcriptome amplification was performed with KAPA HiFi Hotstart Readymix PCR kit (Kapa Biosystems, Cat: KK2602) and SMART PCR Primer (Supplementary Data) ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR was executed using a 384-well block on a LightCycler 480 system (Roche, Switzerland). Relative transcript levels for Pink1 and Prkn were calculated with 2-ΔΔCT [46] using Actb as housekeeping gene and normalized to the relative expression level of the corresponding tissue from 3-month-old mice ...
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bioRxiv - Cell Biology 2024Quote: ... The Quantitative real-time PCR was performed with a Roche LightCycler 480 Instrument II (Roche diagnostics), using 2µl of 10 times diluted cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification of first-strand cDNAs was performed on the LightCycler 480 Real-Time PCR system (Roche), using Light cycler 480 SYBR Green I Master mix 2X (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification of first-strand cDNAs was performed on a LightCycler 480 Real-Time PCR system (Roche) in 96-well plates at 95 °C for 2 min and 45 cycles of 95 °C for 10 s and 56 °C for 60 s ...
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bioRxiv - Neuroscience 2024Quote: ... Riboprobes were generated by PCR and labelled with the DIG RNA labelling kit from Roche (11175033910). The next day ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... The qRT-PCR reactions were performed employing a Mini Option real-time detector (Roche LightCycle@480). Each reaction mixture comprised 10 µL of Talent qPCR Premix (2×) ...
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bioRxiv - Cell Biology 2020Quote: ... and analyzed by qPCR using the KAPA SYBR Green 2x PCR master mix (Kapa Biosystems, Wilmington, MA) in an Eppendorf Realplex 2 Mastercycler according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPA SYBR FAST Universal qPCR Kit (KAPA BIOSYSTEMS) in a QuantStudio3 system (Life Technology) ...
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bioRxiv - Microbiology 2021Quote: The cds1 ORF was PCR amplified from genomic Mtb DNA using KOD Xtreme HotStart DNA polymerase (Roche) according to the manufacturer’s protocol and primers (Rv3684F and Rv3684R) ...
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bioRxiv - Microbiology 2019Quote: ... The library was quantified prior to loading using quantitative real-time PCR (KAPA Biosystems, Wilmington, MA, USA) on a CFX96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... probes and TaqMan master mix were mixed and PCR was run in a LightCycler® 480 (Roche). The mean cycle threshold (Ct ...
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bioRxiv - Synthetic Biology 2022Quote: ... and internal sites removed via PCR using KAPA HiFi HotStart DNA Polymerase with 2X Master Mix (Roche), or synthesized (Integrated DNA Technologies) ...