Labshake search
Citations for Roche :
751 - 800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The colorimetric reaction was performed by adding NBT/BCIP reagents (Roche) to the alkaline phosphatase buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... For qPCR LightCycler 480 SYBR Green I Master mix (Roche Diagnostics, Vilvoorde, Belgium) was used in final reaction of 15 μL ...
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bioRxiv - Microbiology 2024Quote: ... Transposon junctions were amplified with oligos pJZ_Fwd_RND1 and olj376 using 500 ng DNA and KAPA HiFi Hotstart Mix (Kapa Biosystems, #KK2602). Reactions were stopped at the inflection point of amplification (6-14 cycles) ...
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bioRxiv - Microbiology 2024Quote: ... The LightCycler 96 Real-Time PCR System (Roche Diagnostics) was used for amplification with the following thermal cycle conditions ...
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bioRxiv - Cell Biology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2024Quote: ... on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were incubated with 2% Dispase II (neutral protease; Roche Applied Science, Indianapolis, IN) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Microbiology 2024Quote: ... The mRNA transcript levels of selected IVSPER genes were measured by quantitative reverse transcription-PCR (qRT-PCR) using a LightCycler® 480 System (Roche) and SYBR Green I Master Mix (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... and SYBR Green I Master Mix (Roche). Expression levels were normalized relative to a housekeeping wasp gene (elongation factor 1 ELF-1) ...
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bioRxiv - Microbiology 2024Quote: ... These libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche). All samples were pooled and run on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The dual barcodes were attached in a second PCR with the following reaction mix using KAPA HiFi HotStart ReadyMix (Roche), using 1 µL of a 1:10 dilution of the initial PCR as a template and 0.3 µM of the barcoding primers (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were exposed to chemiluminescence detection films (Roche Diagnistics, Rotkreuz, Switzerland). Detection of anti-actin served as loading control for the lysate.
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bioRxiv - Molecular Biology 2024Quote: ... beads were collected using a magnet stand at 4 °C and washed 3 times with beads wash buffer (sperm dilution buffer supplemented 1x cOmplete EDTA-free protease inhibitor cocktail (Roche, # 4693132001), 1x PhosSTOP (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Ethylenediamine tetraacetic acid -free protease inhibitor cocktail (Roche) was added fresh to the binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... RNaseA treated samples were then mixed with 1 μL of 19 mg/ml Proteinase K solution (Roche) and incubated at 55 °C for overnight ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... qPCR analysis was performed using LightCycler 480 SYBR Green I Master Mix (Roche) using primers listed in Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2024Quote: ... completed with protease and phosphatase inhibitors (Roche #11697498001 and #5892970001), to isolate the nuclei from the cytoplasm ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR reactions were carried out on 0.625 ng of cDNA using a LightCycler 480 System (Roche), with the SYBR® Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2024Quote: Total RNAs were isolated using TriPure Isolation Reagent (Roche). For reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche) equipped with a 100W xenon excitation lamp ...
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bioRxiv - Cancer Biology 2024Quote: ... human 293FT cells were grown on 10cm dishes and transfected with sgRNA-containing vector (pX330A-1x2-cNAT10/PITCh) and donor vector using Xtremegene 9 transfection reagent (Roche). 72 h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were validated using the Agilent TapeStation and quantified by using Qubit Fluorometer as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were multiplexed and clustered on a flowcell ...
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bioRxiv - Molecular Biology 2024Quote: ... containing Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... . Apical wash and basolateral samples were assayed following protocol instructions (Cytotoxic Detection Kit, Roche Applied Science). To calculate absolute values ...
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bioRxiv - Microbiology 2024Quote: ... L-lactate dehydrogenase (Roche Applied Science) was used to construct a standard curve that demonstrated an ample linear dynamic range (r2>0.998 ...
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bioRxiv - Microbiology 2024Quote: ... and CDT genes was determined by quantitative reverse transcriptase PCR (qRT-PCR) in a LightCycler 480 thermal cycler (Roche Diagnostics, USA). The primers specific for toxin A ...
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bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cycling was carried out in a Lightcycler (Roche), and GLuc mRNA levels determined by the ΔΔCt method using 18s RNA as reference gene (18s F ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Cell Biology 2024Quote: ... and organoids were resuspended in RIPA protein lysis buffer containing protease inhibitor cocktail (Roche) at day 7 ...
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bioRxiv - Microbiology 2024Quote: ... and DNA ligations were performed using the Rapid DNA Ligation kit (Roche).
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bioRxiv - Microbiology 2024Quote: ... supplemented with cOmpleteTM EDTA-free protease inhibitors (Roche) as previously described (13) ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Microbiology 2024Quote: ... in a LightCycler® 480 system (Roche Molecular System), using primers and probes targeting the M gene of influenza A (sequences available upon request ...
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bioRxiv - Microbiology 2024Quote: ... Typhi harboring the different transcriptional reporters were grown at 37 °C in LB supplemented with glucose-6-phosphate (G6P) (Roche) (concentrations ranging from 7.6 μM to 10 mM ...
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bioRxiv - Cell Biology 2024Quote: ... and PhosSTOP (Roche). Protein concentration was calculated by using the BCA Assay (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Schizonts were then lysed in the presence of 0.15 % saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (Roche) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...
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bioRxiv - Microbiology 2024Quote: ... The prepared libraries were then quantified using KAPA Illumina library quantification kit (Roche) and run on a LightCycler 480 real-time PCR instrument (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... and run on a LightCycler 480 real-time PCR instrument (Roche). The quantified libraries were then multiplexed and the pool of libraries was then prepared for sequencing on the Illumina NovaSeq 6000 sequencing platform using NovaSeq XP v1 reagent kits (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in TBS containing 5% BSA (Roche, 10735086001) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed in blocking buffer with anti-HA-tag (rat, Roche, 11867423001, 1:250) or anti-CD9 (mouse ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked in 5% BSA (Roche, 10735086001) in PBS solution ...
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bioRxiv - Cancer Biology 2024Quote: ... with LightCycler® 480 SYBR Green I Master (Roche, cat. no:04 707 516 001) was used to perform quantitative PCR under following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCRs reactions used KAPA HiFi HotStart ReadyMix (Roche) as per manufacturer’s instructions using an annealing temperature of 65.7C.
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bioRxiv - Cell Biology 2024Quote: ... 1x protease inhibitors cocktail (Roche) and 1x phosphatase inhibitors cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2024Quote: ... the transfected cells underwent initial selection with Hygromycin B (Roche, 10843555001) and were subsequently sorted into single colonies in 96-well plates via flow cytometry.
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (Roche, 11814460001 or abcam, ab290), anti-GAPDH (Proteintech ...