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Citations for Roche :
901 - 950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... DNA was amplified using the 2X KAPA Taq ReadyMix PCR Kit (KAPA Biosystems, now Roche Sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM EDTA pH 8.0) with cOmplete™ protease inhibitor (Roche 4693159001) and Phosstop™ phosphatase inhibitor (Roche 4906845001) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phosstop™ phosphatase inhibitor (Roche 4906845001). The mixture was homogenized 40x using a Dounce homogenizer on ice ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... with oligo-p(dt)15 (Roche®, 10814270001). The cDNAs were analyzed by real time PCR using SYBR Green (Bimake ...
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bioRxiv - Immunology 2024Quote: ... using SYBR Green I master mix (Roche). Equal amounts of cDNA were used to assess relative expression of uncle fester compared to ef1α using the following exon spanning primers ...
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bioRxiv - Immunology 2024Quote: Quantitative Polymerase Chain Reaction (qPCR) was performed on a LightCycler480 (Roche) using SYBR Green I master mix (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0) with protease inhibitors (Roche). Insoluble fraction in lysate was spinned down at 12,000 rpm for 15 min at 4°C to discard ...
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bioRxiv - Molecular Biology 2024Quote: ... The detection was done using DIG Luminiscent Detection Kit (Roche) following the recommendations of the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... in H2O] and incubated with In Situ Cell Death detection kit (Roche) used according to manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... they were purified in large volume columns using the High Pure Viral Nucleic Acid Large Volume Kit (Roche) with 2.5 mL of PB buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for high throughput sequencing were made by PCR using KAPA HiFi HS ReadyMix (Roche) from genomic DNA (Qiagen blood and tissue kit ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was amplified using the 2X KAPA Taq ReadyMix PCR Kit (KAPA Biosystems, now Roche Sequencing, Indianapolis, IN) for 35 cycles using an annealing temperature of 64°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was used for qRT□PCR analyses using the TransStart® Green qPCR SuperMix kit (AQ101-01, TransGen Biotech, Beijing, China) on a LightCycler® 480 System (Roche, Basel, Switzerland). Ubiquitin 5 was selected as an internal control ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA libraries were prepared using the Transcriptor First Strand cDNA Synthesis Kit (Roche). For RT-qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries are prepared by KAPA HyperPrep Kit (Roche, 07962347001). DNA sequencing was performed on the Illumina HiSeq 2500 or HiSeq X.
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bioRxiv - Genomics 2024Quote: ... and one EDTA-free protease inhibitor tablet (Roche) per liter of culture) ...
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bioRxiv - Genetics 2024Quote: ... 1X PhosSTOP (Roche Cat. # 04906837001), 1 mM NaF ...
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bioRxiv - Neuroscience 2024Quote: ... Blood glucose levels were measured using glucometer (Accu-Chek, Aviva, Roche) every week by fasting mice for 2–3h and then withdrawing a drop of blood from the tail vein ...
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR (qPCR) analysis was conducted on a LightCycler480 II (ROCHE), and the qPCR data were normalized to the reference genes UBC and PP2A ...
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bioRxiv - Molecular Biology 2024Quote: ... containing protease inhibitor cocktail (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The KAPA HiFi HotStart ReadyMix (Roche) was used with primers P5_tracrRNA_fwd and P7_tracrRNA_rev and the following cycling conditions ...
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bioRxiv - Molecular Biology 2024Quote: PCR-based amplification of sgRNA regions was performed as described previously27 with minor modifications - KAPA HiFi HotStart ReadyMix (Roche) was used for PCR1 and PCR2 with primers listed in Table S2 and following cycling conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... and 400nM 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP, Roche).
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bioRxiv - Developmental Biology 2024Quote: ... 0.1% tween) and stained in alkaline tris buffer with 550nM Nitro blue tetrazolium chloride (NBT, Roche), and 400nM 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR reactions were set up in technical duplicates using LightCycler 480 SYBR Green I Master Mix (Roche), 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... on a Light Cycler Real-Time PCR System (480II, Roche). The primer sequences (Shanghai Generay Biotech Co. ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was obtained using Proteinase K (3115887001, Roche, Basel, Switzerland) digestion followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Neuroscience 2024Quote: ... The assay was performed using a LightCycler480 (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (Roche; #11583816001), anti-Phospho-IκBα Ser32 (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... 1% IGEPAL complemented with 1x of reconstituted 10x Complete Mini Protease Inhibitor Cocktail Tablet (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (Roche; #11583816001), anti-β-actin (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed for 10 min on ice in 3 ml ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1, 1 mM PMSF, 1,5x Roche protease inhibitor mix). The DNA was sheared by sonication (4x 7 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... protease inhibitor cocktail tablet (Roche; #11873580001; solved in ddH2O), DTT (Serva ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Molecular Biology 2024Quote: ... sequencing grade enzyme (Roche) per sample and tryptic peptides were desalted using C18 Sep-Pack Cartridges (Waters) ...
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bioRxiv - Immunology 2024Quote: ... lungs were digested with DNase (Roche 4536282001) and Liberase (Roche 5401119001 ...
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bioRxiv - Immunology 2024Quote: ... and Liberase (Roche 5401119001) solution at 37°C for 1h and then smashed using a 70µm Cell Strainer filter (Corning 352350) ...
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bioRxiv - Neuroscience 2024Quote: ... 1X proteinase inhibitor cocktail (Roche, #11697498001). The tissue lysate was frozen for 20 minutes at -20°C ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared by KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems, MA, USA) as previously described (Grabowska et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... 1 x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... pHL7.4) with the addition of protease and phosphatase inhibitors (1X protease inhibitor mixture (cOmplete, Roche), 1LmM NaF ...
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bioRxiv - Neuroscience 2024Quote: ... ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablets (plus protease inhibitors) (Roche). For experiments where separate measurements were made in ganglia versus neurites ...
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bioRxiv - Neuroscience 2024Quote: ... 1 x protease inhibitor (Roche, 11873580001), RNAse inhibitor (0.4 U/ul ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were incubated in ice-cold RIPA lysis buffer (Thermo) supplemented with an EDTA-free protease inhibitor cocktail and phosphatase inhibitor (Roche). Plates were placed in a rocking platform in the cold room for 20 minutes and scraped ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5 mM spermidine along with phosphatase and protease inhibitors (Roche). Homogenization was performed on ice ...