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Citations for Roche :
951 - 1000 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... and run on LightCycler 96 Systems (Roche). Relative gene expression was quantified by normalizing cycle threshold values with 18S rRNA using the 2-ΔΔCt cycle threshold method ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... 10% glycerol and a cocktail of protease inhibitors (Roche, Basel, Switzerland). Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... with protease inhibitor cocktail (Roche) and 1 mM phenylmethanesulfonylfluoride added ...
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bioRxiv - Immunology 2024Quote: ... using a LightCycler (Roche) as described previously (18) ...
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bioRxiv - Molecular Biology 2024Quote: ... MEFs were frozen in liquid nitrogen and stored at 80 °C or directly lysed with lysis buffer (50 mM HEPES pH 8.0, 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: Samples were lysed in RIPA buffer containing protease inhibitor (Roche) and Laemmli buffer containing 2.5% of β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail, 1x Roche PhosSTOP) for 1h on ice by agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phosstop™ phosphatase inhibitor (Roche 4906845001). The mixture was homogenized 40x using a Dounce homogenizer on ice ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... with oligo-p(dt)15 (Roche®, 10814270001). The cDNAs were analyzed by real time PCR using SYBR Green (Bimake ...
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bioRxiv - Immunology 2024Quote: ... using SYBR Green I master mix (Roche). Equal amounts of cDNA were used to assess relative expression of uncle fester compared to ef1α using the following exon spanning primers ...
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bioRxiv - Immunology 2024Quote: Quantitative Polymerase Chain Reaction (qPCR) was performed on a LightCycler480 (Roche) using SYBR Green I master mix (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0) with protease inhibitors (Roche). Insoluble fraction in lysate was spinned down at 12,000 rpm for 15 min at 4°C to discard ...
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bioRxiv - Molecular Biology 2024Quote: ... The detection was done using DIG Luminiscent Detection Kit (Roche) following the recommendations of the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... and cOmplete Protease Inhibitor Cocktail (Roche). Cell lysate was mixed at 1:100 (v:v ...
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bioRxiv - Molecular Biology 2024Quote: ... at a ratio of 6:1 using lipofectamine (Roche, catalog #6365787001), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% Glycerol and cOmplete protease inhibitor EDTA-free (Roche, Germany)) were added and cells were sonicated using an MS72 tip on a Bandelin Sonopulse device with 52% amplitude for 15 s on ice ...
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bioRxiv - Immunology 2024Quote: ... 1 unit of Liberase TL (Roche) and 0.6 mg DNAse enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... cOmplete protease inhibitor EDTA-free (Roche, Germany) and incubated with 1 ml of cleared input lysate overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using a LightCycler 96 with SYBR Green (Roche) and gene-specific primers (Supplemental Table S2) ...
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bioRxiv - Immunology 2024Quote: ... Plasma triglycerides were measured with Cobas p 312 (Roche).
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bioRxiv - Immunology 2024Quote: ... all from Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of DNase I (Roche) treated RNA was converted to cDNA using BioScript Reverse Transcriptase (Bioline ...
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bioRxiv - Genomics 2024Quote: ... Each qPCR reaction was assembled with FastStart Universal SYBR Green Master (Rox) (Roche Cat. No. 4913850001) and 2 µl of undiluted cDNA sample in 10 µl total volume ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.5 mM spermidine and 1X complete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11836170001). The nuclei were then further processed with a loose-fit Dounce homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche) and left on ice for 10 mins ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was amplified using the 2X KAPA Taq ReadyMix PCR Kit (KAPA Biosystems, now Roche Sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM EDTA pH 8.0) with cOmplete™ protease inhibitor (Roche 4693159001) and Phosstop™ phosphatase inhibitor (Roche 4906845001) ...
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bioRxiv - Immunology 2024Quote: ... 1X EHF buffer with 15 mM MgCl2 (Roche), 1 U HiFi enzyme mix (Expand High Fidelity PCR System ...
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bioRxiv - Molecular Biology 2024Quote: Labelling of probes with digoxigenin was done using the 2nd generation DIG Gel Shift EMSA kit (Roche, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed again and visualized by Lumi-Light Plus detection reagent (Roche, Basel, Switzerland).
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Immunology 2024Quote: ... 0.025 mg/ml DNAse I (#11284932001, Roche), and 0.5 mg/ml Collagenase D (#11088866001 Roche ...
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bioRxiv - Immunology 2024Quote: ... and 0.5 mg/ml Collagenase D (#11088866001 Roche) for lungs or (ii ...
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bioRxiv - Plant Biology 2024Quote: ... and anti-GFP antibody (Roche).
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were resuspended in lysis buffer (100 mM Pi pH 7, 100 mM NaCl, 1 mM DTT, C0mplete protease inhibitor tablet, Roche) and lysed by sonication for 8 min (Vibracell ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... by measuring released LDH (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR assays were prepared using KAPA SYBR FAST qPCR kit (Kapa Biosystems) and run on an Applied Biosystems QuantStudio 12K QPCR instrument ...
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bioRxiv - Plant Biology 2024Quote: ... AV2 RNA was detected with the digoxigenin (Roche Diagnostics GmbH) labelled in vitro transcribed RNA fragment reverse complementary to the AV2 CP ORF ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on a 480 LightCycler thermocycler (Roche, Indianapolis, IN, USA) following the manufacturer’s instructions with TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Plant Biology 2024Quote: ... Probes were synthesized using the DIG RNA Labeling Kit (Roche, Mannheim, Germany) for CDS of the FLC gene cloned into the pGEM®-T Easy vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... a LightCycler® 480 (Roche) and the primers described below ...
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bioRxiv - Plant Biology 2024Quote: ... using commercial SYBR green mastermix (Roche). Samples were subjected to 2 min pre-incubation at 95°C followed by 40 amplification cycles of 15 s at 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... the volumes of each reagent used were as follows: FastStart Universal SYBR Green Master (Roche) 5.50 μL ...
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bioRxiv - Plant Biology 2024Quote: ... 100 nM of each forward and reverse primer (Supplementary Table S4) and 125lllng λ-DNA (Roche Diagnostics, Vilvoorde, Belgium) was mixed with 2lllμL of a 12x diluted sample cDNA ...