Labshake search
Citations for Roche :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: Snap-frozen samples were lysed with ASBA lysis buffer (61) containing protease inhibitor cocktail solution (Roche), followed by mechanical homogenization (5x 10sec) ...
-
bioRxiv - Neuroscience 2024Quote: ... and RNase inhibitor (Roche) in 1x reaction buffer (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... and DIG-11-UTP (Roche), treated with DNaseI (Promega) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequence-specific primers and probes for each gene were procured from Roche Applied Science ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
-
bioRxiv - Neuroscience 2024Quote: ... AAV titer was determined by qPCR to normalize titers between different viral vector batches using LightCycler 480 SYBR Green I Master (Roche 04707516001). Primer for qPCR targeting the synapsin promoter were as follows ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% Triton X-100) supplemented with protease inhibitor (Roche miniComplete) and cleared by centrifugation at 1,400 x g for 10 min at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The cDNA produced was diluted 1:2 for use with LightCycler 480 SYBR Green I Master (Roche) with the primers listed in the section above ...
-
bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
-
bioRxiv - Neuroscience 2024Quote: ... and PhosStop (Roche 04906837001) phosphatase inhibitors ...
-
bioRxiv - Neuroscience 2024Quote: ... / 0.1 mg/ml Liberase TL (Roche) / 20 μg/ml DNase I (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... and X-tremegene HP (ROCHE) was used to transfect the plasmid ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% NP-40) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche; Basel, Switzerland). Lysates were incubated for 10 minutes on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
-
bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2024Quote: ... The membranes were exposed to chemiluminescence detection films (Roche Diagnistics, Rotkreuz, Switzerland). Detection of actin served as loading control for the lysate.
-
bioRxiv - Microbiology 2024Quote: RNAs were extracted 48 hours after RNP transfection using the High Pure RNA Isolation Kit (Roche) according to instructions of the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
-
bioRxiv - Neuroscience 2024Quote: ... and a phosSTOP-phosphatase inhibitor cocktail tablet (4906837001 Roche – Merck, Darmstadt, Germany). Brain region lysates were incubated for 10 minutes on ice and centrifuged for 10 minutes at maximum speed at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne) and analyzed using a Lightcycler 480 system (Roche). Fold changes were calculated using the double ΔCt method ...
-
bioRxiv - Neuroscience 2024Quote: ... Fluorescein (Roche-11684795910) at 37 °C for one hour ...
-
bioRxiv - Neuroscience 2024Quote: ... DIG-probes were synthesized with a labeling kit according to the manufacturer’s instructions (Roche, France). In situ hybridization was performed according to a modification of (Tiveron et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... The initial PCR of rbcL F3R3 was performed in a reaction mixture of 12.0 μl with 6.0 μl of KAPA HiFi (Kapa Biosystems, Wilmington, MA, USA), 0.7 μl of 10 μM primer mix for rbcL F3R3 (S1 Table) ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 7.8) containing protease inhibitor cocktail (Roche) for 10 strokes ...
-
bioRxiv - Neuroscience 2024Quote: hiPSC-DANs cell pellets were collected in ice-cold PBS and were homogenised in RIPA buffer containing protease (cOmplete EDTA-free, Roche) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Neuroscience 2024Quote: ... and phosphatase inhibitors (PhosSTOP, Roche). Protein concentration was determined by BCA assay (Thermofisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
-
bioRxiv - Neuroscience 2024Quote: All qPCR reactions were run on a LightCycler 96 (Roche, 05815916001) using a QuantiNova Probe PCR Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 mM NaF and 2 mM Na3VO4) containing a protease inhibitor cocktail (Roche). The cell lysates were clarified by centrifugation at 13,000 rpm for 15 min at 4 °C and then denatured with SDS-PAGE sample buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... and phosphatase inhibitors (Roche). Equal amounts of protein lysates were used to incubate with pre-washed anti-HA magnetic beads (ThermoFisher ...
-
bioRxiv - Neuroscience 2024Quote: ... BSA (2mg/ml, Roche), and 1X SSC ...
-
bioRxiv - Neuroscience 2024Quote: ... Triton 0.01X) supplemented with a protease inhibitor cocktail (#11873580001, Roche Diagnostic). Samples were then sonicated (2 ×3 s ...
-
bioRxiv - Neuroscience 2024Quote: ... alkaline phosphatase-conjugated antibody (Roche). The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate ...
-
bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
-
bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT and protease inhibitor cocktail (Roche), then lysed in a high-pressure homogenizer (EmulsiFlex-C5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
-
bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
-
Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... and libraries were generated using the KAPA Hyper Prep Kit for DNA (Roche, Basel, Switzerland) for sequencing on a NOVAseq S4 flow cell (Illumina) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1× complete proteinase inhibitor cocktail (Roche, 11873580001)) 41 and sorted by Becton Dickinson FACS Aria III flow cytometer (Becton ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was incubated with LysB equilibrate Ni+ beads (Roche cOmplete Resin) for 1h at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... transferred to nylon membrane (Roche) and UV-crosslinked using standard manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: NB was performed according to the manufacturer’s protocol (DIG Northern Starter Kit, Roche). Digoxigenin (Dig)-labeled antisense riboprobes were produced using using T7 RNA polymerase by in vitro transcription with RiboMAX Large Scale RNA Production Systems (Promega) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.1 mM DTT) containing EASYpack Protease Inhibitor Cocktail (Roche). Following homogenization ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2x cOmplete™ EDTA-free protease inhibitor (Roche, Mannheim, Germany), 10 µM MG132 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% Triton and 1x cOmplete solution (Roche). Total protein concentration was measured via Bradford assay ...
-
bioRxiv - Neuroscience 2024Quote: ... and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt, Roche). After development ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
-
bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...