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Citations for Merck :
2101 - 2142 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were grown in M9 medium (Green et al., 2012; Meyer and Schlegel, 1983) containing (15NH4)2SO4 (Sigma/Merck KGaA, Darmstadt, Germany) or (15NH4)2SO4 and 13C-D-glucose (Euriso-Top GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
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bioRxiv - Microbiology 2020Quote: ... the bacteria were inoculated into the same medium (0.2% v/v) and the cell density was monitored using a Guava EasyCyte flow cytometer before harvesting (Merck Millipore, Billerica, MA, USA). Cell pellets were harvested from 10 ml culture by centrifugation which were stored at −80°C prior to lipid extraction.
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Microbiology 2021Quote: ... Five milliliters of each standard solution was placed in a flat-bottom test tube for spectrophotometer analysis (Spectroquant Empty cells 16 mm; Merck KGaA, Darmstadt, Germany), which was also used for incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020). For biochemical experiments ...
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bioRxiv - Neuroscience 2023Quote: Cerebellar organoids were generated as previously described (Silva et al 2020) with some alterations: 80-90% confluent iPSCs were dissociated into single cells using Accutase (Merck, Cat. no. A6964), and 4,500 cells were seeded per well of 96 well V-bottom low adhesion plates (S-bio ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with freshly made SA-β-gal buffer containing 25 mM Na2HPO4 (Merck Life science), 7.4 mM Citric Acid (Merck Life science) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Cancer Biology 2023Quote: 1000 freshly sorted GFP+ and GFP- MM13 and MM27 cells were grown as hanging drops in IMDM complete medium (Sigma Aldrich-Merck, Cat#I3390) for 72 h at 37°C in normal (20%O2 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Neuroscience 2023Quote: ... filtered to remove cell debris and particles were concentrated (30-fold) via low-speed centrifugation (Amicon Ultra-15, Ultracel-100; Merck Millipore; Cat# UFC9100) before aliquoting and storage at −70°C ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cell Biology 2022Quote: The cellular health of Eca EPCs Non-OB and Eca EPCs OB was evaluated using Guava® Muse® Cell Analyser (Sigma-Aldrich/Merck, Poznan, Poland). The metabolic activity of cells was determined based on mitochondrial membrane potential using Muse™ Mitopotential Assay Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies were added to each aliquot (equivalent to 100 ml of cell culture): 6μl anti- H3K56ac for the mid-S time point (Merck-Millipore, 07-677-IS (lot# 266732) or 10 μl anti- H3K56ac for the early-S time point (Active Motif ...
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bioRxiv - Neuroscience 2019Quote: The level of RET phosphorylation in the cells was analyzed by Western blotting of immunoprecipitated RET with phospho-tyrosine-specific antibody (1:1500 Merck Millipore Cat# 05-321, RRID:AB_309678) as described previously (Leppanen et al. ...
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bioRxiv - Cell Biology 2024Quote: ... rat neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL poly-D-lysine (PDL, Merck/Sigma-Aldrich #A-003-E). Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020) ...
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bioRxiv - Biochemistry 2023Quote: ... Fifty μl of a suspension in water of GA-fixed cells were mixed with 50 μl of 2% osmium tetroxide in water (Cod. 75632-5ML, Merck Life Science S.r.l., Milan, Italy). After 30 min at room temperature ...
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bioRxiv - Physiology 2023Quote: The pancreatic β cell line INS-1E was cultured in a humidified chamber with 5% CO2 in RPMI-1640 medium (Merck, Sigma Aldrish, Darmstadt-Germany) at 11.1 mM glucose supplemented with 5% (vol./vol.) ...
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bioRxiv - Cell Biology 2024Quote: ... reducing RAD2l levels about 40% relative to our CTCF-EGFP line (using Simple Western of asynchronous cell lysates, RAD2l detected via anti-RAD2l antibody (0S-908, Merck Millipore, l:S0, Suppl. Fig. 4G). 0verexpression of RAD2l rescued this effect ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were dissociated by mincing and enzymatic digestion of the extracellular matrix using 500 µg/ml collagenase type 1a (Merck Life Sciences UK Ltd, Gillingham, U.K) and 100 µg/ml DNAse I (Lorne Laboratories Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Microbiology 2020Quote: ... as previously described (details in Supplementary materials).13–16 Organoid-derived monolayers (ODMs) were prepared in Matrigel-coated transwell cell culture inserts (Merck Millipore, 0.6 cm2, 0.4 μm pores). For this ...