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Citations for Merck :
1701 - 1750 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... exponentially growing RPE-1 cells were pulse-labeled with 1 μM CldU (5-Chloro-2’-deoxyuridine, Merck, C6891) and 250 μM IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice with PBS and quenched with 50 mM Glycine (in PBS, Merck Life science) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... they were co-cultured with HB-82 cells in absence or presence of 5 ng/mL tacrolimus (Merck) in an E:T ratio of 1:1 for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Medium for UWB+ B1 cells was supplemented with 200 μg/ ml G418 S (Merck, Kenilworth, NJ, United States) to maintain the transfection ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells were thawed in the presence of benzonase (Merck 70746-3, used at 1µl/ml), cells were counted and up to 3 x 106 cells were processed for mass cytometry ...
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bioRxiv - Microbiology 2023Quote: ... mixed with ∼5 x 105 Jiyoye cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich/Merck) and spun at 800 g for 2 h ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 96 well plates (6055302, Revvity) and incubated with 100 nM Bafilomycin A1 (Merck, B1793) or vehicle (DMSO ...
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... This sequence was inserted into the pET-HisTEV vector and transformed into Rosetta2 (DE3) cells (#71397, Merck Millipore) to express N-terminally His-tagged Praja1 ...
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bioRxiv - Cancer Biology 2024Quote: Adhesion assay to different recombinant ECM proteins was performed using colorimetric ECM Cell Adhesion Array Kit (ECM540, Merck) plating 3×104 cells/well at 48h post-siRNA-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... DNA from cells of each genotype was isolated using the GenElute Mammalian Genomic DNA Miniprep Kit Protocol (Merck) and sequenced using the BovineSNP50 v3 DNA Analysis Bead Chip (Illumina ...
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bioRxiv - Immunology 2024Quote: ... cells after 12 days of IVS with OMPs pools were collected and plated in ELISpot plates (Merck Millipore) pre-coated with a monoclonal anti-IFN-γ mAb (clone 1-D1K purified MabTech ...
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bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were resuspended in ice cold PBS containing 0.1 % Triton X-100 and protease inhibitor cocktail (Merck, 4693116001), in order to extract the cytoplasm ...
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bioRxiv - Molecular Biology 2020Quote: ... cell culture supernatant of HUVECs was concentrated to 1 mL using Amicon-Ultra 15 Centrifugal Filter Units (UFC901096, Merck) and incubated with 0.5 µg of Alexa 488 labeled anti-CD9 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was used to transduce naïve Huh7 cells in the presence of 10 μg/ml polybrene (Merck Millipore). After 72 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin was isolated from cell lysates and enzymatically fragmented using an EZ-Zyme Chromatin Prep Kit (Merck 17 375). 10 ug of anti - AR antibody (Abcam ab74272 ...
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bioRxiv - Immunology 2019Quote: ... Approximately 4×105 of the cultured cells were then transferred to each well of 96-well filter (Merck Milipore) pre-coated with 2 µg/ml TT in 1x PBS or only 1xPBS ...
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bioRxiv - Immunology 2021Quote: ... cells were re-suspended in FACS buffer prior to analysis using a GUAVA EasyCyte benchtop flow cytometer (Merck Millipore) equipped with the relevant laser and filters to detect FITC fluorescence ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed 2x 4 min in PBS and subsequently overlaid with 0.1 mg/ml collagen IV (C7521, Merck) and placed at 4°C overnight (ON) ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Neuroscience 2020Quote: ... After 20 days all the collected media was pooled and concentrated/buffer exchanged to PBS (1X, pH 7.4) using an Amicon stirred cell (Merck) with a 3 kDa molecular weight cut-off membrane.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were washed three times with PBS and saved in PBS supplemented with 0.02% sodium azide (Merck, S2002) at 4°C until image acquisition.
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptotic cells were identified by TUNEL assay on sections using an ApopTag in situ apoptosis detection kit (Merck-Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for SA-β-galactosidase activity overnight using a Cellular Senescence Assay kit (Merck Millipore, Massachusetts, USA) and imaged the next day in the motorised widefield microscope Cell Observer (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... and plated in glass-coverslips (3000-4000 cells/well) pre-coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured on poly-L-ornithine and laminin-coated coverslips in 24-well plates at 150,000 cells per well in serum-free 2i Medium (Merck). After 24h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed with 1x Laemmli (Bio- Rad Laboratories) with Benzonase nuclease and phosphatase inhibitors (Sigma-Aldrich p2850, Merck), and the lysates were migrated in 4–15 % gradient SDS-PAGE gels (Bio- Rad Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... when colony dispersion was observed and the cells were fixed and colored by Hemacolor® stain (Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in 48-well plates (3000-4000 cells/well) previously coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Neuroscience 2020Quote: ... SH-SY5Y wildtype (Accession Nr: CRL2266), and SH-SY5YΔPRNP (Pease et al., 2019)) were cultured in 150-cm2 Corning cell culture flasks (Merck) and counted using Trypan Blue (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... For electrophysiology experiments the excretory/secretory extracts were dialysed/buffer exchanged to PBS using an Amicon stirred cell (Merck) with a 3 kDa molecular weight cut-off membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell extracts (500 μg per IP) were immunoprecipitated for 1h using a PHGDH-specific antibody (Merck Sigma, HPA021241) complexed with Dynabeads Protein A (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... we harvested the medium from the bottom compartment and counted the number of migrated cells using a Bürker-Türk counting chamber (Merck, BR719505). After cell counting ...
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bioRxiv - Microbiology 2022Quote: The growth of asexual blood stages was assessed by infecting NMRI mice at a parasitaemia of 0.01% and counting infected red blood cells in blood smears stained with Hemacolor (Merck). The percentage of total gametocytes was also determined by counting in blood smears ...
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bioRxiv - Immunology 2022Quote: ... Cells were then diluted to 200 μl with cold PBS and acquired using a GUAVA flow cytometer (Merck Millipore). Propidium Iodide (PI,Sigma ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral supernatants were generated into HEK293T cells by transient co-transfections of lentiviral CAS9-RFP Lenti Plasmid (Merck, USA) construct and appropriate amount of packaging vectors (Mission Lentiviral Packaging Mix ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...