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Citations for Merck :
2001 - 2050 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with a BG4-Flag antibody (1:100 diluted in 0.5% goat serum + PBST, MABE917, Merck, Darmstadt, Germany) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the heart was removed and cells were dissociated and filtered through a 70 µm filter after which BSA (Merck Life Science) was added to a final concentration of 4% to inactivate the enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The pNGS2-N12-BC libraries were prepared by purification from the harvested cells by using either GenElute Plasmid Miniprep (Merck, Germany) or NucleoBond Xtra Midi (TaKaRa Bio Inc. ...
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bioRxiv - Genetics 2023Quote: Wild type and PREPL KO HEK293T cells were grown on glass coverslips coated with Poly-D-Lysine (Merck A-003-E) and fixed with 4% formaldehyde ...
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bioRxiv - Immunology 2024Quote: ... cell lysates of macrophages were removed and the infected Vero cells were overlaid with the Vero culture medium containing 0.5% methylcellulose (Sigma-Aldrich; Merck KGaA) for 4 days ...
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bioRxiv - Genomics 2024Quote: ... 8 x 106 HeLa-Cas9-p65-mNeonGreen cells 10 were transduced with 1 ml lentivirus library and 10 μg/ml polybrene (Merck Millipore). After one day ...
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... cells were split into either 6 well-plates (300000 cells per well) coated with 1 mg/ml Poly-L- Lysine (PLL, Merck, P9155) for western blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X Protease inhibitors and 1X Phosphatase inhibitors (PPI)] plus 2μL per sample Pierce Universal Nuclease for Cell Lysis and 1μL per sample Benzonase® Nuclease (Merck, E1014-25KU) for 30min at 4°C on wheel with vigorous pipetting every 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The purity of the cultures was be routinely assessed by examining the characteristic cell morphologies under phase-contrast microscopy and confirmed by immunostaining with mouse anti-GFAP (1:200; Merck, MAB3402) and mouse anti-S100β (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... and the trophozoites were resuspended in 500 μl NaPBS/0.05 % saponin (or permeabilization of cell membranes (Sigma-Aldrich, Merck, Taufkirchen, Germany) and incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM medium (Merck/Biochrom) supplemented with 10% HI-FBS (Merck/Biochrom) ...
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bioRxiv - Bioengineering 2023Quote: ... CellBiologics) were cultured in a complete Endothelial Cell Medium (CellBiologics) in petri-dish pre-coated with 0,2% gelatin (Merck Life Science). Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were grown until confluence in 0.2 mg/ml collagen-type I-coated 6 well plates (Merck, Darmstadt, Germany) and incubated in serum-free medium for 24 h with/without Dox treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... feeder depleted mESCs were seeded for EB formation at 25,000 cells/ml in ESGROTM Complete Basal Medium (ESGRO, Merck, SF002-500) in non-adhesive 60 mm or 100 mm culture dishes ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Immunology 2023Quote: Bovine cytokines and chemokines secreted in the supernatant by cells were measured using the commercial multiplex immunoassay MILLIPLEX MAP Bovine Cytokine/Chemokine Panel 1 (Merck/Sigma). Therefore ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue fragments (‘organoids’) released were incubated for 5 min in Red Blood Cell Lysis buffer (Merck Sigma-Aldrich, Gillingham, Dorset, UK), washed and then plated for 1 hr at 37°C in DMEM/10%FBS (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Microbiology 2024Quote: Human small lung carcinoma A549-AT cells stably expressing ACE2 and TMPRSS2-GFP73 and VeroE674 cells were maintained in Dulbecco’s Modified Eagle’s High Glucose Medium (DMEM, Merck, Cat. no. D6546), 1% L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were then washed in cold 50 mL cold PBS cOmplete™ EDTA-free Protease Inhibitor Cocktail tablets (Merck, #73567200) and centrifuged at 2000 rpm for a further 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Microbiology 2024Quote: ... algal cells concentrations from a late exponential phase culture were counted by a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) using 561 nm excitation and 702 nm emission ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the SH-SY5Y cells were collected and the ROS levels were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells were incubated with 5 µM DHE dye for 20 min at 37°C in the dark and then the fluorescence intensity was measured by flow cytometry in the PE channel ...
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bioRxiv - Genomics 2020Quote: ... 10 × 106 cells were used per IP and all lysis and wash buffers were supplemented with Roche cOmplete™ protease inhibitors (Merck, 11873580001) and Pierce phosphatase inhibitors (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were allowed to adhere overnight (16 h) in the presence of 2 mM Thymidine (S-phase block; Merck, Darmstadt, Germany) and released into fresh medium ...
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bioRxiv - Bioengineering 2020Quote: ... the digests were separated by an Amicon® Bioseparations Stirred Cells device over a 1kDa ultrafiltration disc (Merck Millipore; Amsterdam, The Netherlands) into a retentate and filtrate ...
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bioRxiv - Microbiology 2019Quote: ... total GLP-1 levels in cell supernatants were measured using High Sensitivity GLP-1 Active ELISA-Chemiluminescent (Merck Millipore, EZGLPHS-35K, Germany) according to the reports (38 ...
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bioRxiv - Genetics 2019Quote: ... Chromosome spreads were prepared by dropping the cell suspension on a grass slide and stained with 5% Giemsa solution (Merck Millipore, Germany) for 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were washed with cold PBS and centrifuged at 2000 x g for 5 minutes and stained using a Muse™ Cell Cycle Kit (Merck Millipore) for 30 minutes in dark conditions ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...