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Citations for Merck :
1851 - 1900 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the iMEF cells were incubated for 7 days with HOT or vehicle and with 100 ng/ml nocodazole (Merck, M1404) in DMSO or with vehicle for 18 h before sample collection ...
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bioRxiv - Cell Biology 2022Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK), HIF2A (Novusbio/NB100-122, Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK) and GAPDH (Merck/MAB374 ...
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bioRxiv - Cell Biology 2022Quote: ... dishes cultured with cells were washed twice with DPBS and stained with 0.25% rhodanile blue (Merck Life Science, UK, #121495) in 1% sulphuric acid/DPBS solution for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The cell medium was aspirated from the wells and fresh medium with one of the factors (Bafilomycin, 200 nM, Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The frozen cell pellets were incubated with 25 mM Tris buffer (pH 7.4) containing 0.05% n-dodecyl-β-D-maltoside (Merck, D4641) and 1 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... after which the filtrate was filtered onto 0.2 µm pore size (free-living cells) polycarbonate filters (Merck Millipore, Darmstadt, Germany). Foam from the Timor Sea (Station 8 ...
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bioRxiv - Microbiology 2020Quote: Live cell fluorescence imaging was performed as previously described 78 with the minor modification of nuclear staining with Hoechst (Merck) instead of DAPI at a final concentration of 5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 20 million cells using the Magna ChIP A/G Kit (One-day chromatin Immunoprecipitation Kits, Merck) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM heroin was diluted in the respective cell medium and sterilized by filtration using a 0.22 μm Millex-GV syringe filter (Merck Millipore). After 20 days differentiation ...
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bioRxiv - Neuroscience 2020Quote: Cell-cycle kinetic differences were assessed by labelling cortical progenitor cells using a nucleotide analog 5-ethynyl-2’-deoxyuridine (EdU; Merck) in vivo following 150 □g EdU injection into the peritoneal cavity of pregnant mice 1 hour before the sacrifice of the embryos ...
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bioRxiv - Microbiology 2021Quote: ... the DNA content or cell sizes were analyzed using the ImageStreamX MarkII Quantitative imaging analysis flow cytometry (Merck Millipore, Germany), which was calibrated with non-labeled beads with a diameter of 2 µm ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Immunology 2020Quote: ... cell culture media were removed and total RNA was extracted from the cells by using 300 μL/well of TRI Reagent (Merck) and Direct-zol RNA minipreps (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: Ago2 RIPs were performed on TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck millipore) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... GGA reactions were performed in a thermocycler according to a standard GGA protocol.52 The GGA products were transformed into NovaBlue cells (Merck), from which the plasmids were purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Biochemistry 2019Quote: ... A single colony of freshly transformed cells was cultured at 37 °C in 500 mL 2xYT medium supplemented with 50 µg/mL kanamycin (Merck) and 25 µg/mL chloramphenicol (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... organoids generated from the H9 doxycycline-inducible ZEB2 (HumiZEB2) cell line were treated with 1.7 μg/ml doxycycline (Merck, PHR1145) during the first 5 days of the protocol.
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bioRxiv - Cell Biology 2019Quote: Hela cells were transiently transfected with pSpCas9(BB)-2A-GFP (PX458)(83) encoding sgRNAs targeting VPS41 using X-tremeGENE (Merck) according to the manufacturers recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... pBADthio-LmaCK1.2ΔC43- V5-His6 and pBADthio-LmaCK1.2ΔN7-V5-His6 were sequenced and transformed in Escherichia coli Rosetta (DE3) pLysS Competent Cells (Merck Cat# 70956) for bacterial expression.
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bioRxiv - Biochemistry 2021Quote: ... Transient transfection of U2OS and HepG2 cells with plasmid DNA was carried out using GeneJuice (Merck Millipore, cat. number 70967) according to manufacturer’s instruction and keeping GeneJuice to DNA ratio of 3:1 (volume in μl ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Microbiology 2021Quote: ... CTR1 KO cells were generated by co-transfecting 293T cells with the pX458 vector carrying the Cas9 protease fused to GFP and a Sanger lentiviral CRISPR vector (Merck) carrying the BFP and a sgRNA against human CTR1 under the control of the U6 promoter (sgRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... iPSCs were maintained according to [71] in 60-mm Petri dishes (0,6 × 105 cells total per dish) coated with 0.1% gelatin (G2625, Merck, Darmastadt, Germany) in Glasgow’s Minimum Essential Medium (GMEM ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Neuroscience 2022Quote: ... microglia were isolated and cultured for 3 days as above before cells were lysed in RIPA buffer (60 μL/well, Merck). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with MISSION® SiRNA oligomers or MISSION® siRNA Universal Negative Control #1 (Sigma Aldrich, Merck, USA) at a final concentration 20 nM ...
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bioRxiv - Immunology 2022Quote: Decidual cells were isolated as described above and incubated in the presence of fluorescence-labeled latex beads (50 beads per cell; latex beads, 1 µm, carboxylate-modified polystyrene, fluorescent yellow-green, Merck) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Infection and selection of stably infected cells were done in the presence of the appropriate concentration of puromycin (Merck-Aldrich), titrated by using the minimum antibiotic concentration sufficient to kill untransfected cells ...
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bioRxiv - Neuroscience 2022Quote: ... cells were replated onto coverglass (8×104 per well) coated with 50 µg/ml Poly-D-Lysine (PDL, Merck, USA) for imaging experiments.
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bioRxiv - Bioengineering 2022Quote: ... were rinsed with PBS and incubated in 500 μL of cell lysis solution containing 0.2% (v/v) Triton-x-100 (Merck, 1.08603.1000) in 5 mM MgCl2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Genetics 2022Quote: ... Transient transfection was carried out in HEK293T cells transfected with 1 μg of plasmid DNA in the presence of X-tremeGene9 DNA transfection reagent (#6365809001, Merck), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Parasitemias were calculated from day 3 post-infection by counting infected red blood cells in blood smears stained with Hemacolor (Merck).
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bioRxiv - Neuroscience 2022Quote: Tissues or cells were homogenized in Pierce IP Lysis buffer supplemented with protease inhibitor cocktail 3 (Merck Millipore, Darmstadt, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Lgr5+ cells in the mice were ablated with a single intraperitoneal dose of Diphtheria Toxin (DT) (Cat # 322326-1mg, Merck) in saline (50 µg kg−1).
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bioRxiv - Cell Biology 2022Quote: Yeast cells were grown to mid-log-phase on SD-Leu media and analyzed on a CellStream analyzer instrument (Merck) using 488 nm and 561 nm lasers for capturing the fluorescence emission of GFP and Cherry ...
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bioRxiv - Neuroscience 2024Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were starved (0% serum) for 24h and then treated for 96h with 5mM Metformin (Merck, cat. nr M0605000) or 2ng/ml TGF-β1 (Peprotech cat nr 10021) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cytospins were prepared by spinning 30.000 cells per slide and stained with May-Grünwald-Giemsa staining (Merck KGaA, Darmstadt, Germany). 2*200 cells were identified by morphology and counted at 20x magnification (Olympus BX51) ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 400 μl of 40 μg/ml ultra-pure digitonin (Merck, CAS 11024-24-1) in vPBS and incubated (25 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck). Cell vials were stored in a liquid nitrogen tank.
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...