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Citations for Merck :
1601 - 1650 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cell proliferation assay was done using the standard MTT assay (Sigma-Aldrich/Merck, Catalog No. 11465007001). MTT assay was performed on each well following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: Treated BMDM cells were lysed with RIPA buffer (supplied with cOmplete™ Protease Inhibitor Cocktail, Merck#11697498001), and protein concentration was determined by Bradford assay ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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Ozone mediates tumor-selective cell death caused by air plasma-activated medium independently of NOxbioRxiv - Cancer Biology 2023Quote: ... The cells were maintained in 10% FBS (Serena Europe Gmbh, Brandenburg, Germany) containing DMEM (Merck, NM, USA) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (FBS/DMEM ...
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bioRxiv - Cell Biology 2023Quote: HEK cells were grown in an 8-well chamber slide (Millicell EZ slide, Merck-Millipore, Burlington, MA) to early confluence ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and fixed for at least 3 h with 70% ethanol (Merck, 100983). Cells were washed with PBS and analyzed by using the Cell Cycle Kit (Luminex Corporation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was assessed by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; S7100, Merck Millipore). Images were obtained with a Leica DM3000 microscope with a mounted Leica DFC420 camera ...
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bioRxiv - Neuroscience 2023Quote: All cell cultures were maintained in 25mM glucose DMEM (Dulbecco’s Modified Eagle’s Medium; catalogue # D5671, Merck, UK) with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was estimated using a propidium iodide stain (2µg/mL; catalogue # P4864, Merck Life Sciences (UK)) as previously published28.
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS+ and incubated twice (15 min each) with 50 mM MESNA (Merck) in 100 mM Tris ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... cell lysates (500 μg) were subjected to immunoprecipitation with LRP1α (1 μg, Cat#: L2295, Merck, Rahway, NJ), HA (0.2 μg ...
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bioRxiv - Microbiology 2024Quote: ... HEK293 cells were transfected with different vectors and exposed to different concentrations of HCA (Merck, Cat#: 700159P) and GW4064 (MCE ...
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bioRxiv - Genomics 2024Quote: ... All protoplasts from each conical flask were then filtered through a 100 µm cell strainer (CLS431752, Merck) into one 50 ml falcon tube and pelleted using a swinging bucket rotor centrifuge at 1,000-2,000 x g for 2-3 minutes at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown on LB agar plates (40g/l LB-agar, 100 µg/ml Ampicillin (Merck A9393)) and single clones were cultivated in LB broth overnight (25g LB ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed twice with PBS and detached by treating with 1 mL Accutase™ (Merck) for 30 min at 37°C in 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were labeled with 20 mM 5-chloro-2-deoxyuridine (CldU, Sigma-Aldrich by Merck, Darmstadt, Germany) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA immunoprecipitation was performed on 100 μl cell lysate per sample using the Magna RIP Kit (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene transcription in CHO cells was induced by adding 100 ng/mL doxycycline (Merck KGaA, Darmstadt, Germany). Cells were incubated at 37℃ in 5% CO2 and 95% air.
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed once with DPBS and stained with 1 μg/ml Hoechst 33342 (Merck, Zug, CH) in a Live Cell Imaging Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cells were harvested for FACS analysis (excitation 488 nm, emission 690/50 nm; Guava easyCyte Flow Cytometer, Merck). Increased red fluorescence has been correlated with the formation of mitochondrial reactive oxygen species (ROS) ...
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bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The number of cells in each sample was counted using a Guava easyCyte 8HT flow cytometer (Merck, Germany). To describe and compare the effect of each chelator ...
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bioRxiv - Neuroscience 2019Quote: ... 25 U/ml Penicillin and 25 µg/ml Streptomycin and 0.455% (v/v) 7.5% NaHCO3 aqueous solution) on Millicell Cell Culture Insert (Merck) at air-medium interface ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Genomics 2019Quote: The populations were enriched in metaphasic cells by 2 h of treatment with 200 nM nocodazole (Merck, M1404) prior to cell recovery ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 h cells were stained for 30 min at 37 °C with NAO dye (200 nM, Merck), washed ...
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bioRxiv - Biophysics 2021Quote: HaCaT mCherry-Histone H2B cells were seeded in 96-well glass bottom plates (Greiner Sensoplate (M4187-16EA, Merck)) at 75 000 cells per well ...
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bioRxiv - Bioengineering 2021Quote: INS-1 cells were stained with red fluorescent membrane-inserting dye PKH-26 (Lot# SLBW0232, Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Each medium collection was concentrated/buffer exchanged to PBS (1X, pH 7.4) using an Amicon stirred cell (Merck) with a 3 kDa molecular weight cut-off membrane ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with 250 μl of PBS and analysed via flow cytometry (Guava easyCyte 5HT; Merck group). 5000 events were recorded per strain and condition using InCyte software (3.1).
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bioRxiv - Neuroscience 2021Quote: ... seeded at a density of 25,000 cells/cm2 on culture plates or glass coverslips previously coated with 0.01% poly-L-lysine (P8920, Merck), and incubated at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 2 twice with PBS and were permeabilized with PBS–0.06%-Triton X-100 (Merck, USA) for 10 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted from the cells 48 h post-transfection by complete lysis-M buffer (Merck, USA). Twenty-five mg of total protein were loaded per well in a 12% SDS PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Microbiology 2019Quote: DF1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Extraction of RNA from cell lines/strains was carried out using TRI Reagent (Merck Life Sciences, Dorset UK) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 16.6 μl/10,000 cells) encoding for ZsGreen transcription in a complex medium containing 5μg/ml polybrene (Merck Millipore). Using qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... The cells at 13 days and 20 days after electroporation were treated with doxycycline (1 µg/mL, Merck) for 24 hours and subjected to microscopic and flow cytometric analyses ...
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bioRxiv - Cell Biology 2022Quote: ... Cells (8 × 104) were seeded on fibronectin (bovine, 0.1 mg/mL) coated Millicell EZ SLIDE 8-Well (Merck) in 300 µL media/well (RPMI + 10% FCS + 1% ultraglutamine + 1% Pen/Strep + 1% HEPES + 1% Na-pyruvate + 1% MEM NEAA + 0.1% mercaptoethanol).
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were isolated from cord blood fractions by density centrifugation using Ficoll (Biocoll, Merck Millipore, Burlington, Massachusetts). Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... Pseudotyped GFP expressing coronaviruses were harvested from the cell supernatant 24 hpi and concentrated using Amicon columns (Merck). Then the viruses were titrated in Vero E6 cells and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were subjected to SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Merck Millipore; Darmstadt, Germany). After blocking the membranes with 5% skim milk (Morinaga ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for one hour at room temperature with a mouse monoclonal antibody against γH2AX (Merck) diluted in PBS/SVF (1/1000 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed using 4% paraformaldehyde and stained with antibodies against ZO-1 (rabbit polyclonal, #617300, Merck-Millipore) and Nestin (mouse monoclonal ...