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Citations for Merck :
1651 - 1700 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cells released from double thymidine arrest were incubated 4 hrs later with CDK1 inhibitor RO-3306 (Merck millipore) at 9µM concentration for 4 hrs ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human bronchial epithelial cell lines Calu-3 and the Vero E6 cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with 10% standardized fetal bovine serum (FBS Superior; Merck), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... virus inoculum was removed from each well and cells were overlaid with 300 μL of 0.6% carboxymethylcellulose (Merck) diluted in DMEM supplemented with 2% FBSi ...
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bioRxiv - Physiology 2019Quote: ... Mice were sacrificed using cervical dislocation and epididymal WAT pads were dissected and placed in explant culture growth medium (Dulbecco’s MEM/Ham’s F12 cell culture medium (FG4815, Merck) containing 1% Penicillin-Streptomycin (5,000 U/mL ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded either on Geltrex coated cover slips or in Millicell® EZ SLIDES (#PEZGS0816, Merck Millipore). Cells were fixed with 4% (vol/vol ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were incubated at room temperature for 20 minutes and analyzed with Muse Cell Analyzer (Merck, Darmstadt, Germany) with 5000 events per sample ...
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bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were harvested in 4°C RIPA buffer with protease inhibitor (cOmplete, EDTAfree Protease Inhibitor Cocktail, Merck) and homogenised by sonication for 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspension was collected by centrifugation and transferred to an activated (by elution of 1mL CH3CN (Merck)) and equilibrated (by elution of 1mL MeOH/H2O (80/20 ...
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bioRxiv - Immunology 2020Quote: ... Primed neutrophils were obtained by treating the cells for 25 mins with 10 μM cytochalasin B (Merck, #C6762) and 100 nM N-Formylmethionyl-leucyl-phenylalanine (fMLF ...
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bioRxiv - Cell Biology 2021Quote: ... or 2500 cells/well depending on PARPi dose in 5 ml of medium supplemented with 1.5% methylcellulose (Merck) and the indicated concentrations of Olaparib ...
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bioRxiv - Cell Biology 2022Quote: Double thymidine block (DTB) – exponentially growing cells were treated with 2.5 mM thymidine (Merck Life Science Ltd 6060) for 16 h to arrest them at G1/early S-phase ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were stained with 1 mL 10% Giemsa’s azur eosin methylene blue solution (Merck KgaA, Darmstadt, Germany) in milli-Q water for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... expanding cells were transferred to erythroid differentiation medium (Iscove’s medium with stable glutamine [Merck] containing 3% (v/v) AB serum [Merck] ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Cancer Biology 2022Quote: Xanthine oxidase activity was measured in 4T1 cells cultured in hypoxia using the xanthine oxidase activity kit (Merck) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... before transduction of TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and fresh media added ...
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bioRxiv - Plant Biology 2023Quote: ... cells were transfected with plasmids encoding the respective heterologous proteins using the Genejuice transfection reagent (Merck, Darmstadt, Germany). After 48 hours of incubation at 37°C at 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... 50µl of cell culture medium was incubated with 100µl of Griess reagent A (1% sulfanilamide (Merck, Darmstadt, Germany) and 5% phosphoric acid (Slavus ...
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bioRxiv - Microbiology 2022Quote: ... The cell mixture was spotted on a 0.45 μm pore-size nitrocellulose filter (Merck, Millipore Ltd., Tullagreen, Ireland) placed on marine broth agar ...
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bioRxiv - Cell Biology 2024Quote: ... whole cell lysates were prepared from two pellets per group using Phosphosafe Extraction Reagent (Merck Millipore, Darmstadt, Germany) containing 1 mM Pefablock SC (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 wild-type U2OS or AsiSI-ER U2OS cells were crosslinked with 1% formaldehyde (Merck, 252549-100ML) for 10 min at 37°C and inactivated by the addition of glycine (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with gene-specific siRNAs or a control siRNA (MISSION siRNA Universal Negative Control #1, Merck) to a final concentration of 45 nM and 400 ng of the respective rescue plasmid with 12 µL of Liopfectamine 2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
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bioRxiv - Microbiology 2024Quote: ... and cell debris was removed by passing it through a 0.45 μm pore size filter (Merck, Cat# SLGVR33RB). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... cells were briefly reactivated by addition of 10 ng/ml PMA and 1 μg/ml ionomycin (all Merck) for 1h prior to flow cytometric analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... cell culture supernatant was concentrated using Amicon filter columns with either 30 kDa or 100 kDa cutoff (Merck), retaining glycodelin in the concentrated part ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with 1 µg (1:100 dilution) of a anti-Rabies Virus CVS-11 antibody (Merck) or anti-VZV gE mouse antibody (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with 50mM ammonium chloride for 10 min and permeabilised with 0.25% Triton X-100 (Merck) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: THP1 cells were seeded into 96 well plates in RPMI containing 100 ng / µl PMA (PMA) ( Merck, P1585) and left to adhere overnight at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... For reporter gene assays 293FT cells were transiently transfected using a slightly modified polyethylenimine (PEI, Sigma-Aldrich/Merck) transfection method72 ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After 23 h the cells were detached and reseeded on glass cover slips coated with Concanavalin A (Merck, Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 5 min with prewarmed PBS containing 12.5 mg/ml N-Ethylmaleimide (Merck Life science) at 37 °C prior to harvesting to fix the cysteine-dependent redox state ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferative potential of the cells was calculated using Alamar Blue Reagent (cat. no. R7017; Sigma-Aldrich; Merck KGaA) at a final concentration of 0.2mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Genomics 2023Quote: ... 1µM PD032552 inhibitors (both from Cell Guidance Systems) and 1000 U/mL units of Leukemia Inhibitory Factor (Merck). Cells were passaged with 0.05% accutase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on the chamber slides were fixed with ice cold methanol (Sigma-Aldrich/Merck Cat. No. 34860) for 5 minutes at room temperature and then gently washed with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections for the generation of stable cell lines were performed using X-tremeGENETM 9 DNA transfection reagent (Merck), following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: Cells of 2-3 days growth with approximately 70-90% confluency have been treated with Bortezomib (Merck, #504314) at 100 nM for 42 h ...
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bioRxiv - Microbiology 2023Quote: ... auris cells (CHRIST Alpha 1-2 LD plus lyophilizer, Osterode, Germany) using Tri Reagent (Merck Ltd. Budapest, Hungary). The quality of RNA was determined using the Eukaryotic Total RNA Nano kit (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Cell nuclei were isolated using a cell lysis buffer (5mM Pipes pH8.0, 85mM KCl, 0.5% NP40, Complete protease inhibitor cocktail (Merck)) for 20min ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transduced with 1 MOI of concentrated virus containing 8 ug/mL Polybrene (Merck, TR-1003-G). Two days later ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated at a density of 0.4 × 105 cells/cm2 on poly-L-lysine (0.1 mg/ml, Merck) coated glass coverslips ...
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bioRxiv - Genomics 2023Quote: ... Slide that now contained stained cells was air dried and mounted with 1ml of 85% glycerol (Merck Millipore) and covered with a coverslip (Menzel-Glaser) ...
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bioRxiv - Immunology 2023Quote: ... Cells were subjected to image acquisition with Image Stream X MKII imaging flow cytometer (Amnis/Merck Millipore/Luminex) and analyzed with IDEAS 6.3 software (Amnis/Merck Millipore/Luminex) ...