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Citations for Merck :
1801 - 1850 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the retina was placed with the RPE facing down on cell culture inserts (Millicell, Cat. No. PICM0RG50, Merck Millipore) with DMEM culture medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the cell pellet was resuspended in PBS-citrate solution supplemented with 0.01% w/v bovine serum albumin (BSA, 10735086001, Merck) and centrifuged (5 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 wild-type or p53 missense mutant cells were transfected with the plasmids using X-treme GENE HP (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were resuspended in a modified sucrose electroporation buffer prepared by adding 272 mM sucrose (Sigma-Aldrich, Merck) to Opti-MEM™ Reduced Serum Medium (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Immunology 2023Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck) according to manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... MPAs or U373 cells were seeded in DMEM (catalogue # 11966) with 7.5mM glucose (catalogue # G7021, Merck Life Sciences (UK)) ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck). After 48 h ...
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bioRxiv - Immunology 2023Quote: Cells were infected separately with five different lentiviral transduction particles (at MOI = 5) containing five different shRNA species (Merck) specific for the mouse Flot2 gene (NM_008028 ...
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bioRxiv - Systems Biology 2023Quote: ... For the multicellular spheroid generation 7.000 cells were seeded on ultra-low attachment 96-well plates (Corning CoStar, Merck). The spheroids were cultured for 72h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... LSEC were isolated by gradient density sedimentation of resting non-parenchymal cells on 25–50% Percoll (Merck Millipore, Germany) and purified by using CD146-based isolation on magnetic MicroBeads (MACS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Genetics 2024Quote: ... The harvested medium was then centrifuged at 1,300 rpm at 4 °C for 5 min to remove cells and then filtered by 0.45 μm filter (Merck) and the virus was collected and stored at -80 °C.
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bioRxiv - Immunology 2024Quote: ... cells were plated on 10-well diagnostic microscope slides (Epredia) coated with 0.1% (w/v) poly-L-lysine (Merck) in H2O ...
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bioRxiv - Neuroscience 2024Quote: ... After perfusion and brain tissue digestion as detailed above, cell pellet was resuspended in MACS buffer (PBS, 0.5% low endotoxin BSA (Merck), 2 mM EDTA ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from cells or tissues by RIPA buffer supplemented with a protease inhibitor cocktail (Merck, #11836170001) and phenylmethanesulfonyl fluoride (Beyotime ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviruses were added to cells together with 6 µg/ml of polybrene (TR-1003-G, Merck Milipore, Darmstadt, Germany) and incubated for 24 hours.
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bioRxiv - Neuroscience 2024Quote: ... Sections or cells were pre-incubated in blocking buffer containing 2–10% normal donkey serum (Merck Millipore, Burlington, MA) and 0.1% Triton X-100 at RT for 1 h and incubated overnight at 4 °C with primary antibodies and subsequently incubated with secondary antibodies conjugated with Alexa Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... Labeled DiO - AEVs (1x1011/well [10 µl]) were added to THP1-b cell monolayers cultured on collagen solution (Merck) coated 12-well chamber slides (IBIDI ...
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bioRxiv - Immunology 2024Quote: B cell lines used as target cell lines (i.e. Raji and MEC1) were grown at 37°C and 5% CO2 in RMPI-1640 medium (Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Biophysics 2020Quote: ... 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 µm pores (Cat # 353096, Merck). The inserts were coated with rat-tail collagen I ...
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bioRxiv - Cell Biology 2020Quote: Epithelial canine MDCK II (CRL2936) cells were obtained from the ATCC and grown in MEM supplemented with 5% FBS (Merck) at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Microbiology 2021Quote: ... and human lung carcinoma (A549) cells (CCL-185) were purchased from ATCC (Manassas, VA USA) and maintained in Dulbecco’s modified Eagle medium (Merck, Germany) supplemented with 10% foetal bovine serum (FB-1365 ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were finely dissected into tiny pieces and enzymatically digested into single cells by incubating them in a dissociation solution containing 0.125 mg/ml Collagenase (Merck, C9891), 1 U/ml Dispase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
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bioRxiv - Plant Biology 2020Quote: Frozen cell pellets harvested from approximately 1 L of suspension cultures were crushed with quartz fine granules (Merck, Darmstadt, Germany) with a precooled mortar and pestle in ice-cold lysis buffer (25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed once and then incubated with the appropriate fluorescently labelled secondary antibody (anti-mouse polyvalent Ig-FITC (Merck) or anti-His6 HIS.H8 DyLight 488 ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Cell Biology 2021Quote: Equal numbers of c-Kit+ bone marrow cells were expanded in the presence of doxycycline for 24 hours and treated with cycloheximide (CHX; 100 µg/mL; Merck) for 8 min at 37 °C before harvesting by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellets were resuspended in PBS+0.5% Triton-X 100 supplemented with Complete™ protease inhibitor cocktail (Merck, USA) and frozen overnight at −80 °C for lysis ...
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bioRxiv - Biochemistry 2020Quote: ... Cell culture supernatants were collected after 48 hr and 72 hr and filtered through 0.22 µm syringe filters (Merck Millipore). Filtered supernatants were stored at -80 °C until further analysis ...
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bioRxiv - Biochemistry 2020Quote: ... The cerebella were removed and sliced into 400 μm sagittal sections using a McIlwain tissue chopper and then plated evenly across two Millicell cell culture inserts (Merck) in six-well plates (two inserts per cerebellum ...
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bioRxiv - Cancer Biology 2021Quote: ... BM cells harvested from moribund mice were cytospun and stained with Giemsa’s azur-eosin-methylene blue solution (Merck, cat.no. 109204).
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bioRxiv - Cancer Biology 2021Quote: ... Apoptotic cell death was analyzed by staining with Annexin V/PI as per the manufacturer’s instructions (APOAF-20TST, Merck Millipore) and analyzed in BD LSR-Fortessa using FACS-Diva software (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... HL-60 and MS-5 cells cultured alone and in co-culture were incubated for 24 hours in 50 μM H2O2 (Merck) complete cell culture medium ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Systems Biology 2020Quote: The constructs containing the TCA cycle enzymes fused to the biotin ligase and a FLAG tag were stably integrated in the Flp-In T-REx HeLa cells using the X-tremeGENE 9 DNA Transfection Reagent (Merck) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus containing media was collected and PUER cells were transduced by spin-infection in the presence of 12 μg/ml polybrene (Merck) at 850 g for 2h ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF7 cells were transduced with lentiviral particles at 75% confluency in the presence of 10 μg/ml polybrene (Merck, Germany). 24h after transduction virus containing medium was replaced with selection medium for the respective expression constructs to start the selection ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
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bioRxiv - Cell Biology 2021Quote: ... The following day cancer cells were serum-deprived for 24h and then all cell lines were treated either with camptothecin 1-10μM (ref. C9911, CPT; Sigma-Merck, Argentina) or DMSO (ref ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... Spleen cells isolated from the mouse with the best serum titre were fused with Sp2/0 cells in the ratio of 5:1 using polyethylene glycol (PEG) 3000 (#817019, Merck). 10 million cells of the fusion mix were combined with 2x 104 BALB/c peritoneal macrophages and seeded in a 96-well plate ...
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bioRxiv - Microbiology 2022Quote: ... and non-expressing (d2EGFP−) cells were seeded into a 96 well plate pre-coated with 1 mg/ml Poly-D-Lysine (PDL, Merck). Aryl hydrocarbon receptor (AhR ...
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bioRxiv - Microbiology 2022Quote: Supernatants harvested from infected Rhileki cells were centrifuged at 3,500 rpm and precipitated with 10% polyethylene glycol 8000 (Merck, Germany) overnight in 4°C ...