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Citations for Merck :
1951 - 2000 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
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bioRxiv - Developmental Biology 2024Quote: ... Citrine+ cells were enriched via FACS using a BD Influx cell sorter with a 70um nozzle into DNA Lobind 1.5mL Eppendorf tubes (Merck, EP0030108051), spun post sort at 400g ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... grown in semi-suspension in 150 mm plates in a mixture of serum free media (ACF CHO medium EX-CELL Merck, CHO ONE media Capricorn Scientific ...
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bioRxiv - Immunology 2024Quote: ... Viral supernatant was filtered and used for transduction of NALM6 and K562 cells through spinoculation together with 8 μg/ml protamine sulfate (Merck). Transduced cells were selected using puromycin (1 μg/ml).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant containing the pre-cleared crude cell lysate was collected by attaching the tubes to a Magna Rack (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: Transfected cells were solubilized in gel loading buffer and analyzed through electrophoresis before being transferred to nitrocellulose membranes (Merck Millipore). Immunodetection was achieved with the primary antibodies (1:1000 in blocking solution) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue slices were dissected under the microscope in ice cold PBS before they were transferred on top of hydrophilic Millicell cell culture inserts (Merck Millipore) into serum-free Advanced Dulbecco’s modified Eagle’s medium F12 supplemented with 0.5% N2 ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lymphocytes were isolated from the spleen and incubated in a tissue culture tube at a cell concentration of 1 × 106/ml in RPMI1640 (Nacalai Tesque) containing lipopolysaccharide (10 μg/ml, Merck KGaA), concanavalin A (3 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed using ice-cold RIPA buffer containing 1% of protease and phosphatase inhibitor cocktail (Sigma Aldrich/Merck, Poznan, Polska). The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoid cells were cultured in self-renewing medium with ROCKi for 8 days before Dox (Doxycycline hyclate, 2 µg/ml, Merck, D9891) and TMP (Trimethoprim ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 100μM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP; Merck Life Sciences, Gillingham, UK) to activate the P2RX7 ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of ZsGreen1-expressing cells (%ZsGreen) and ZsGreen1 mean fluorescence intensity (MFI) of each sample were analyzed using Guava ExpressPlus software (Merck Millipore) and MFI was expressed as fluorescence arbitrary units (FAU) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... detached cells were then added to phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA) to a final concentration of 10 μM and dissociated to single cells ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... 72 hours conditioned-media from cancer cells was collected and filtered with a 0.45 μm filter (Millex, Merck Millipore, Massachusetts, USA) before adding to fibroblasts ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were dissolved in 1 ml of PBS and nuclei were stained with propidium iodide (PI) (Merck Millipore, Burlington, MA, USA) and analyzed using a flow cytometer (Guava PCA) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50% confluent cells were plated and allowed to adhere for at least 24 hours before treatment with 1 μM 4OH-TAM (Merck, H7904) or with identical volume of EtOH for the indicated time ...
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bioRxiv - Systems Biology 2022Quote: Yeast cell culture pellets were resuspended in lysis buffer composed of 100 mM triethylammonium bicarbonate (TEAB) (Merck Sigma, Cat. No. T7408) containing 0.1% ...
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bioRxiv - Molecular Biology 2022Quote: Antibody-bead mixture was washed twice in iCLIP lysis buffer to remove unbound antibody and RNase-treated cell lysates were added alongside cOmplete EDTA-free Protease Inhibitor Cocktail (Merck, #11836170001) and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Neuroscience 2021Quote: ... These slices were explanted onto Millicell cell culture inserts (pore size 0.4 µm, diameter 30 mm, Merck Millipore Inc., cat# PICMORG50). Membrane inserts were placed into a six-well plate with 1 mL of culture medium ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... USA) cells were transduced with lentiviral particles at 70% confluency in the presence of polybrene (TR-1003-G, Merck, Darmstadt, Germany). 24 h after transduction virus containing medium was replaced byG-418 sulfate (#M3118.0050 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Neuroscience 2022Quote: ... To fix the cells they were washed once with warm PBS and incubated for 20 min at 37°C with 4 % paraformaldehyde (Merck Millipore). After washing ...
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bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 min at 37 °C and washed with 5% FBS (EmbryoMax® ES Cell Qualified FBS, ES-009-B, Merck) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... the medium was changed to normal growing medium and cells were treated with the following compounds and concentrations: Alpha-amanitin (#A2263-1MG, Merck AG): 1 ...
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bioRxiv - Cell Biology 2021Quote: ... human iPSC cells were detached using Accutase for 5min at 37 °C and followed dissociated into single cells with phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA). Cells were centrifuged at 300 rpm for 5 min and the pellet was resuspended in hESC medium (20% knockout serum replacement ...
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bioRxiv - Microbiology 2020Quote: ... and the proportion of EGFP-positive cells was determined every day by flow cytometry using a Guava EasyCyte Flow Cytometer (Merck Millipore). The difference of the percentage of EGFP negative cells between induced and non-induced conditions were plotted as a function of time of growth ...
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bioRxiv - Immunology 2021Quote: ... 20 μl of this cell/collagen mixture was added in one well of pre-cooled Corning™ 96-Well half-area plate (Merck) and the plate was centrifuged ...
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bioRxiv - Immunology 2022Quote: Cells were lysed by addition of a mild lysis buffer containing 1% octyl-β,D-glucopyranoside (#O9882-500MG, Sigma-Aldrich, Merck), 0.25% sodium deoxycholate (#1065040250 ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Synthetic Biology 2022Quote: Cells were grown in CellASIC ONIX microfluidic plates for haploid yeast cells in media controlled by the ONIX2 microfluidics system (Merck, Germany). Details regarding the composition of the media used for different promoter induction experiments are given in Supplementary Note 1.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cancer Biology 2022Quote: ... HIC1 KO cells were seeded on coverslips and DNA damage was induced by incubation with 10 μM etoposide (Sigma-Aldrich/Merck) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... removed and faux adherent cells from the lower membrane washed into the bottom well before migrated cells were counted using a Guava EasyCyte 8HT Flow cytometer (Merck Millipore). Data was then presented as the total number of migrated cells or relative to vehicle controls (% control).
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bioRxiv - Genetics 2022Quote: In vitro differentiated 18 d erythroid cells stained with Hoechst 33342 were resuspended with 150 μl DPBS for analysis with Imagestream X Mark II (Merck Millipore). Well-focused Hoechst-negative single cells were gated for circularity analysis with IDEAS software ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...