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Citations for Merck :
1751 - 1800 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... cells were filtered and resuspended from the filters (MF™ Membrane Filters 0.45 µm, Merck KGaA, 64293 Darmstadt, Germany) in the same volume of synthetic medium without serine ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proportional volumes of bacterial and yeast cells were mixed and filtered through 0.45 µm mixed cellulose membrane (Merck, Millipore) before transferring onto IM agar supplemented with 200 µM acetosyringone ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was removed by centrifugation and the supernatant was filtered through a 0.22 µm syringe filter (Merck Millipore). Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... room-temperature and slightly dried SabCG media plates and a sterile glass L-shaped bacteriology cell-spreader (Merck, S4522) was used to spread the liquid evenly over the entire surface and allowed to soak/dry before use.
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bioRxiv - Microbiology 2020Quote: A 100μL bacterial suspension of 2.0 × 106 cells/mL was prepared and loaded on the four-chambered CellASIC ONIX B04A-03 microfluidic platform (Merck). Cells were trapped with the following pressure and flow time settings ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were dissected free in cold HBSS and flat mounted on cell culture inserts (Millicell 0.4 μm pore; Merck) ganglion cell layer up ...
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bioRxiv - Cancer Biology 2021Quote: ... cells and barcoded beads were respectively diluted at the concentration of 200 cell/μL in PBS with BSA 0.01% (Merck) and 120 bead/μL in lysis buffer ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... control cells were generated using the SHC202 -MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid (Merck). After infection ...
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bioRxiv - Immunology 2020Quote: ... with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 at 4°C at a density of 105 cells/m in starvation media (RPMI (Merck), containing 0.5% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Microbiology 2021Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... live cell fluorescence microscopy was performed as described before (85) with minor modification using 5 μg/ml Hoechst (Merck) to stain the nuclei ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the obtained 2-cell stage embryos were transferred to a fresh drop of KSOM (Merck, cat. # MR-107-D). The IVF with b6d2F1 oocytes and b6casF1 has on average a 2-cell development (fertilization ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were kept for viral titer determination and the cell pellets were harvested by Tri-reagent (Merck, Israel) for RNA purification.
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bioRxiv - Molecular Biology 2022Quote: Chicken embryonal fibroblast cells (DF1; a kind gift from Dr Dylan Sweetman) were expanded in standard Dulbecco’s Modified Eagle Medium (DMEM; Merck Life Science UK Limited ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells not set aside for surface antigen staining were incubated in PBS containing 0.1% Triton X-100 (X100, Merck) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were fixed on 4% PFA and senescence determination was made by measuring SA-β-gal activity (KAA02 Merck) and IF against the markers of senescence ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with lentiviral particles in the presence of polybrene (8 g/mL, Cat# TR-1003-G, Merck); after 48 hours of transduction ...
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bioRxiv - Microbiology 2022Quote: ... and human embryonic kidney (HEK)293T cells (ATCC #CRL-3216) were maintained in Dulbecco’s Modified Eagle Medium with high glucose (Merck), supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: Hela Kyoto and FERRY subunit knock-out cells were cultured in DMEM media supplemented with 10% FBS Superior (Merck) and 50 μg/ml streptomycin (P/S ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured for at least 5 passages in DMEM containing either L- arginine and L-lysine (Merck) or L-arginine [13C6] and L-lysine [2H4] (Cambridge Isotope Laboratories).
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was then lysed in 5X volumes of Lysis Buffer (300μL) [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 cells were treated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Merck Life Science NV) for 24 hours in a T75 culture flask ...
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bioRxiv - Cancer Biology 2024Quote: ... reflecting cell-number and -viability) was assessed using the MTT [Thiazolyl Blue Tetrazolium Bromide] assay (Sigma/Merck, M5655-500mg) according to manufacturer’s instructions and sigmoidal dose-response curves were generated to calculate the mean IC50 values of each drug that were used in the subsequent study ...
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we re-analyzed microarray-based transcriptome datasets: i) HepG2 cells treated with vehicle (0.0125% DMSO) or 12 μg/ml silymarin (Merck) for 24 h (n = 3 biological replicates)98 ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated at a final density of 1 × 104 cells/cm2 on fibronectin-coated (MM Merck Millipore, FC010) µ-Slide 8 Well Glass Bottom Chamber Slides (Ibidi ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed once with PBS and then incubated with RPMI containing 1 µg / ml propidium iodide (PI) (Merck) and nigericin (Merck ...
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bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
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bioRxiv - Immunology 2024Quote: ... For FACS sorted cells RNA was amplified cDNA synthesized using a SeqPlex RNA Amplification kit (Merck Life Science, UK) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
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bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.
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bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were settled in a 96-well half area microplate with clear flat bottom (Corning/Merck) at a density of 25,000 cells per well at RT for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml, Merck, Cat.N. 3072) for the duration of the experiment ...
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation and pellets were suspended in PBST with 250 μg/mL lysozyme (Merck, Darmstadt, Germany) and lysed by three subsequent freeze-thaw-cycles ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Genetics 2023Quote: ... The screens were performed without feeder cells in M15 medium supplemented with 1000 U/ml leukemia inhibitory factor (Merck). Cells were plated on flasks coated with 0.1% gelatin solution (Merck) ...
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bioRxiv - Immunology 2023Quote: ... cell lines were cultured to a density of 70-90% on 150mm Corning tissue-culture treated culture dishes (Merck). For HLA ligandome LC-MS ...
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bioRxiv - Microbiology 2023Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
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bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Merck 200-664-3). None of the cell lines were authenticated but low passage numbers were utilized.