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Citations for Merck :
1551 - 1600 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The cells were maintained in 293T medium composed of DMEM high glucose (4.5 g/l; Merck, NJ) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: The cells were grown in an 8-well chamber slide (Millicell EX slide, Merck Millipore, Burlington, MA) to early confluency ...
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bioRxiv - Cell Biology 2022Quote: KELLY cells were treated with 100 μM 5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB, Merck, D1916-10MG) for 3 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 600,000 cells were lysed using lysis buffer containing 50% of TruPAGE™ LDS Sample Buffer (PCG3009, Merck) and 20% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... at a density of 25×103 cells/cm2 and cultured in EndoGro medium (Merck Millipore, Darmstadt, Germany). TEER was measured daily and experiments were performed when it reaches over 30 Ωcm2 (5-7 days after seeding) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed another 2 times and permeabilised with 0.1% Triton™ X-100 (Merck, UK) diluted in PBS and incubated at room temperature for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: The cells used in this study were adherent and cultured in Corning® T75 flasks (Merck, UK) at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... exponentially growing yeast cells (OD600 0.4-0.6) were loaded in commercial microfluidic chips (CellASIC ONIX2, Merck Millipore) as previously described 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were grown for 48h in serum-free ESGRO Complete Clonal Grade Medium (Merck, Cat# SF001- B) supplemented with 1000 U/ml LIF ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated in the presence of 100 μM allylthiourea (ATU; ≥98% purity, Merck KGaA, Darmstadt, Germany), which has been shown to inhibit comammox Nitrospira previously (2) ...
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bioRxiv - Neuroscience 2020Quote: ... individual EBs were transferred to transwell membranes (Millicell Cell Culture inserts, PTFE, 0.4 μM, Merck Millipore, USA) and allowed to further develop at the air-liquid interface ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed before fixation by treating cells with Tyrode’s acidic solution (Merck, T1788-100ML) as described previously (52) ...
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bioRxiv - Cancer Biology 2022Quote: ... Resistant cells were sorted after TROP2 induction with DOX (final concentration 2 µg/ml; purchased from Merck) by surface labeling with TROP2-specific antibodies (see further).
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bioRxiv - Cell Biology 2022Quote: ... allowing the interaction to be observed as PLA foci within the cells (Adopted from Duolink PLA, Merck). PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated for 36 hours in the presence or absence of PU-WS13 (Merck Millipore). S ...
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bioRxiv - Microbiology 2020Quote: ... the end-point cell lysis staining assay consisted of adding Neutral Red dye (Merck KGaA, Darmstadt, DE) to cell monolayers ...
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bioRxiv - Microbiology 2021Quote: ... According to the manufacturer’s manual cells were loaded into a CellASIC-microfluidic plate type B04A (Merck Milipore) and mounted on a Delta Vision Elite microscope (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Calu-3 cells were transduced by addition of lentiviral supernatants containing 8 μg/ml polybrene (Merck Darmstadt). 48 hours after transduction ...
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bioRxiv - Neuroscience 2019Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Genetics 2020Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... the cells at a density of 5×105 /ml were supplemented with 2% dimethyl sulfoxide (DMSO; Merck) and the culturing was continued for another 24 to 72 hr ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was cleared of cell debris by filtering through a 0.45µm filter (Corning, Merck, Darmstadt, Germany) and mixed with 1x PEG solution (SBI System Bioscience ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Neuroscience 2021Quote: ... After 24h cells were lysed in RIPA buffer containing 10μg/ml protease and phosphatase inhibitor cocktail (Merck). Lysates were cleared by centrifugation and stored at −20°C until use.
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Immunology 2021Quote: Madin–Darby canine kidney (MDCK) cells were cultured in Eagle’s minimum essential medium (MEM, Merck, Feltham, UK) supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS ...
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bioRxiv - Immunology 2020Quote: ... the cells were further cultured for 4 days in the same conditions plus Dexamethasone (1 μM; MERCK). IDCs were equally generated in 4 days ...
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bioRxiv - Bioengineering 2022Quote: Unfixed U2OS cells were incubated in a prewarmed solution of 0.2% Saponin (#47036, Sigma-Aldrich now Merck) in Cytoskeleton Buffer (CB ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was extracted from cells using the GenElute mammalian Total RNA miniprep extraction kit (Merck, RTN70-1KT) and was reverse transcribed to synthesise cDNA using High Capacity cDNA Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Recipient cells were incubated for eight hours with lentivirus and selected three days later with puromycin (Merck) or G418 (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: was measured using Corning® Transwell® 8μm pore polycarbonate membrane cell culture inserts (Merck, Cat# CLS3422). 1×106 cells in serum-free RPMI medium were added to the inserts with RPMI containing 10% FBS serving as chemoattractant in the bottom chamber ...
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bioRxiv - Cell Biology 2022Quote: ... cells were quickly but gently resuspended in six-well coated plates with Synthemax (Sigma-Aldrich; Merck KGaA) in MtesR-plus medium supplemented with CloneR (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and seeded onto coverslips that were coated with 25 µg/ml laminin (Merck, #11243217001) in laminin coating buffer (50mM TRIS ...
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... cells were incubated with an IF-optimized EMRE antibody (anti-SMDT1/C22orf32/EMRE; #HPA060340, 1:250; Merck), washed 3 times in PBS ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed and permeabilized with a 3:1 mixture of methanol (Klinipath)-glacial acetic acid (Merck) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Transfections were carried out in 6 well plates with cells at 50–70% confluency using GeneJuice (Merck) with 1 μg of total plasmid DNA per well ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cell extracts were concentrated using Amicon Ultra-15 3 kDa cutoff (Merck Millipore, Burlington, MA, USA). The obtained cell extract was flash-frozen in liquid nitrogen and preserved at −80 °C until further use.
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bioRxiv - Molecular Biology 2022Quote: ... Expression of gene of interest was induced by treating cells with 1 μg/ml doxycycline (Merck, D9891) for 72 hours and confirmed by western blotting.
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bioRxiv - Molecular Biology 2022Quote: Cells pellets were resuspended in 200μl lysis buffer (8M urea, 50mM Tris pH 7.5, protease inhibitors (Merck)) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed with 3.7% formalin and methanol and stained with Giemsa and May-Grünwald (Merck), as previously described (68) ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Cell Biology 2023Quote: ... 2.5 million cells were dissolved into 100 µl of ICP-OES grade 65% HNO3 acid (Merck #1.00441.1000) and was kept at 95°C for overnight ...
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bioRxiv - Genomics 2024Quote: ... then washed twice in fluorescence-activated cell sorting (FACS) buffer (PBS supplemented with 0.5% BSA (Merck, 10735086001) and 2mM EDTA (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The stained cells were analyzed using the ImageStream X Mark II Imaging flow cytometer (Merck Millipore, USA) with an X60/0.9 objective ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 107 cells were lysed in 200 μL lysis buffer [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... cells were twice washed with PBS and permeabilized with 0.1% TX-100 (in PBS, Merck Life science). Cells were washed an additional 2 times ...
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bioRxiv - Cell Biology 2023Quote: ... pps1Δ cells were freshly grown on YES medium plate supplemented with 0.4 mM ethanolamine (Merck, 398136-25ML) before liquid culture ...