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Citations for Merck :
1501 - 1550 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were incubated with directly conjugated fluorescent antibodies in PBS/1% (w/v) BSA (Merck)/0.05% (v/v ...
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bioRxiv - Cell Biology 2023Quote: Cultured HAEC cells were stained for SA-β-galactosidase according to the manufacturer’s protocol (Merck Millipore). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Anterior and posterior samples were dissociated into single cell suspensions using Liberase TM enzyme (Merck 5401119001) as described in 49 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and incubated as described above and supplemented with 50 nM RSL3 (Merck, #SML2234) and 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Cell extracts were filtered using an Amicon Ultra centrifugal filter unit (10-kDa cutoff; Merck Millipore) and washed four times with buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were infected with lentiviruses carrying target sequences above with 10 μg/ml polybrene (Merck). After 2 h ...
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bioRxiv - Immunology 2023Quote: ... Bacterial cells processed via ultrasound and insoluble inclusion bodies were extracted with 1% deoxycholic acid (Merck) and 1% Triton X-100 (Merck ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with either of the following substances: 10 mM LiCl (Merck KGaA; Darmstadt, Germany), 100 μg/mL rhWnt-3a ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed 1x with PBS and extracted by addition of 1 mL TRI reagent (Merck) directly to the monolayer ...
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bioRxiv - Neuroscience 2023Quote: ... Non-adherent cell culture plates were prepared with poly(2-hydroxyethyl methacrylate) (poly-HEMA; P3932, Merck) coating ...
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bioRxiv - Cell Biology 2023Quote: ... cells were released in EdU 40μM in the presence or absence of aphidicolin (2μM, Merck Millipore) for 40 minutes (for prometaphase cells ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with branched polyethylenimine (bPEI, 10 μM stock solution, Merck, Sigma-Aldrich, Darmstadt, Germany) when 90% confluency was reached ...
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bioRxiv - Cell Biology 2023Quote: ... MCF7 cells were infected in the presence of polybrene (1:2000, Merck Millipore #TR-1003-G). After 24 hours incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and an equal number of cells were digested in 100 μl of Nitric acid (Merck #1.00441.1000) overnight at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three more times and affixed to slides with Fluoroshield histology mounting medium (Merck). Cell were viewed on am IX83 inverted microscope (Olympus ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged at around 80% confluency using 1 mL/well Accutase™ (A6964, Merck, UK) and dissociation was stopped with 4 mL/well ADM ...
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bioRxiv - Immunology 2023Quote: ... adherent cells were trypsinized and frozen in fetal bovine serum (FBS, Merck, St. Louis, MO, USA) with 10% DMSO (Merck ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass slides (Merck). After treatments cells were washed with PBS (3 times ...
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bioRxiv - Microbiology 2023Quote: ... Vero/hSLAM cells (PHE) were grown in DMEM with 10% FBS and 0.05mg/ml gentamicin (Merck) with the addition of 0.4mg/ml Geneticin (G418 ...
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bioRxiv - Cancer Biology 2023Quote: ... neural cells were stained with rabbit polyclonal anti-tyrosine hydroxylase antibody (Merck, AB152, 1:200 dilution), guinea pig polyclonal anti-DCX antibody (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... and the pelleted cells were resuspended in PBS containing 20µg/ml propium iodide (Merck Life science).
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the GenElute™ Bacterial Genomic DNA kit (Merck) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... NSCs were plated at 10,000 cells/cm2 in culture plates pretreated with poly-D-lysine (Merck) and laminin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with filipin complex (Merck, cat #F-9765; 0.15 mg/ML in D-PBS) for 2 h and after several washes in D-PBS were visualized by fluorescence microscopy ...
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bioRxiv - Cell Biology 2023Quote: Hela Kyoto or U2OS cells were grown on coverslips coated with Poly-D lysine (Merck, #P7280). Coverslips containing cells were gently washed twice with 1X PBS to remove excess media ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 hour with 10 µg/mL Calcofluor White M2R (CFW) (Merck KGaA). Cells were harvested by centrifugation (2,000 ×g ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Microbiology 2024Quote: The cells in 96-well plates were fixed with 4% paraformaldehyde (PFA, Merck, Cat. no. P6148) in phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2024Quote: pCold-DDX43 and pCold-DDX6 were transformed into Rosetta 2 (DE3) competent cells (Merck Millipore/Novagen). The cells were cultured at 37°C until the OD600 reached ∼0.6 ...
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bioRxiv - Genetics 2024Quote: ... Jurkat and THP-1 cells were grown in Roswell Park Memorial Institute Medium (RPMI, Merck #R8758). Both were supplemented with 10% fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... supplemented with Benzonase Safety Plus Emprove Expert (0.5 µL/g of wet cell pellet; Merck, #1037731010), and mechanically disrupted using an Avestin Emulsiflex-C5 (3 passes at 500-1000 bar) ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured with 50 U/ml of rhIL-2 in RPPMI-1640 medium (#8758, Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone ...
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bioRxiv - Immunology 2024Quote: ... cells were plated using the same conditions and treated with 6.6μM staurosporine (Streptomyces) (InSolution, Merck #569396) and incubated at 37°C and 5% CO2 for the indicated time.
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was prepared and protein concentration was measured by Direct Detect® spectrometer (Merck). An equal amount of proteins (50 µg ...
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bioRxiv - Cancer Biology 2024Quote: ... cells have been lysed using 2× Laemmli buffer supplied with 100mM DTT (Dithiothreitol, Merck Sigma Aldrich), boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico ...
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bioRxiv - Microbiology 2024Quote: ... the cells were cultured in Grace insect complete medium containing G418 (200 μg/mL) (Merck, Germany), and the culture medium was changed once every 3 d ...
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bioRxiv - Microbiology 2020Quote: ... cells were passed through a 0.1 micron polycarbonate filter (Whatman nucleopore track-etch membrane, Merck, Southampton, UK) directly following the initial fixation and then processed as described above.
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bioRxiv - Biophysics 2021Quote: ... ER-Hoxb8 cells were cultured in uncoated six-well culture plates in RPMI 1640 medium (Merck Millipore) supplemented with 10% FBS (Pan Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was discarded and cell pellet resuspended in 1.8 ml ice-cold 50 mM CaCL2 (Merck, 1.02083.0250), incubated on ice for 20 min and centrifuged for 2 min at 5000xg at 0°C ...
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bioRxiv - Microbiology 2019Quote: ... Cells were fixed with 4% paraformaldehyde (Intron, Seongnam, Republic of Korea) and 100% methanol (Merck, Darmstadt, Germany). The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon ...
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bioRxiv - Developmental Biology 2020Quote: ... the number of viable cells was counted using the Muse Count & Viability Assay Kit (Merck Millipore MCH100102) and run on the Muse cell analyser (Merck Millipore) ...
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bioRxiv - Immunology 2021Quote: ... Inhibition of the TLR-4 pathway was achieved by treating cells with 2 µM TAK-242 (Merck) for 6 hours before adding particles ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the embedded cells were lysed in a lysis buffer containing 2.5M NaCl (Merck Milipore, Burlington, MA, USA), 1 mM Na2EDTA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... These cell lines were routinely tested for mycoplasma infection and treated with BM-Cyclin (Merck, Burlington, USA) to eliminate mycoplasma ...
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bioRxiv - Neuroscience 2020Quote: ... cells were lysed and subjected to subcellular fractionation using a ProteoExtract Subcellular Proteome Extraction Kit (Merck, #539790) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were allowed to attach for 24 hours and then tetracycline (0.13 μg/mL, Merck, T7660) was added in the medium of the stable cell lines for the induction of protein expression ...
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bioRxiv - Microbiology 2021Quote: Yeast cells were digested at 90°C for 4 hours in 65% (w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 48 h after transfection in 2x Laemmli buffer containing 200 mM dithiothreitol (DTT, Merck). Lysates were sonicated and separated by SDS-PAGE/immunoblotting using 7,5 % gels ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated for 40 hours with the pan-FGFR inhibitor PD173074 [100nM] (Calbiochem, Merck Eurolab, France). Control cells were treated with DMSO vehicle diluted proportionally to the inhibitor ...