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Citations for Merck :
1401 - 1450 of 2142 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and Accutase dissociation reagent (97% StemPro® Accutase® Cell Dissociation Reagent (Merck-Millipore, #SCR005), 0.025% Trypsin (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: ... SK-N-BE cells expressing LAMP1-eGFP are treated with 10 µg/mL nocodazole (Merck) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... after which cells were lysed by resuspending in 50 μL of BugBuster reagent (Merck, 71456) and incubation under constant rotation for 10 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... the cells were passaged using 0.02% EDTA Ca2+/Mg2+-free PBS pH 7.2-7.4 (Merck) once 80% confluency was reached ...
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bioRxiv - Pathology 2023Quote: Buccal cells were smeared on glass slides and fixed by M-FIX spray (Merck Millipore). They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242 ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... Transfected cells were collected after three days and suspended in Hank’s Balanced Salt Solution (Merck). The AAV-containing cell lysate was treated with 250 U/ml benzonase at 37 °C for 30 min without adding MgCl2 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 30 min at 4°C with 0.4 % paraformaldehyde (PFA, Merck KGaA) and permeabilized by washing twice with permeabilization buffer (PBS containing 2 % FBS and 0.1 % saponin (Sigma)) ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were fixed and stained with a 0.5% Crystal Violet (Merck-SIGMA, Cat# C6158) in 20% Methanol for 1hr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured at low passage numbers and passaged consistently using Trypsin-EDTA 0.25% (Merck) and tested regularly for mycoplasma contamination (all cells used for experiments were mycoplasma negative at the time of testing).
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bioRxiv - Cell Biology 2023Quote: ... cells were detached with 0.5g/l trypsin and 0.2g/l ethylenediaminetetraacetic acid solution (EDTA) (Merck), for experiments ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with PBS and lysed with 1X RIPA buffer (Merck Millipore #20-188) supplemented with a protease inhibitor cocktail (Thermo Scientific #87785) ...
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bioRxiv - Cell Biology 2023Quote: ... and an equal number of cells were digested in 100uL of Nitric acid (Merck #1.00441.1000) overnight at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with 3% PFA (paraformaldehyde, cat#158127, Merck Millipore, Burlington, MA, USA) for 10 min and subjected to immunofluorescence ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: Analysis of apoptotic cells was performed by dual staining with FITC-conjugated Annexin V (Merck) and propidium iodide (PI ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... Quantification of viable cells was performed by staining with 7-aminoactinomycin D (7-AAD; Merck) and flow cytometry or with crystal violet staining with colorimetric detection.
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bioRxiv - Neuroscience 2024Quote: ... in 0.1 M PB for 45 min to permeabilise cells and with 1% H2O2 (Merck) in 0.1 M PB for 20 min to block endogenous peroxidase ...
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bioRxiv - Neuroscience 2024Quote: ... EBs were dissociated and cells were plated on poly-D-lysine/laminin-coated dishes (Merck) and cultured in neural differentiation medium for 30 days.
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bioRxiv - Microbiology 2023Quote: ... Cells were then fixed and permeabilized using a combination of 4% paraformaldehyde (Sigma-Aldrich/Merck) and 0.1% Triton X-100 (Sigma-Aldrich/Merck) ...
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bioRxiv - Pathology 2023Quote: ... 48h after transfection rAAV8 vectors were harvested from cell lysate and treated with Benzonase (Merck) at 100U/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 80-90% confluent iPSCs were dissociated into single cells using Accutase (Merck, Cat. no A6964) and 9000 cells were seeded per well of 96 well V-bottom low adhesion plates (S-bio ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the cells were plated on 15% Poly-L-Ornithine (Merck/Sigma A-004-C) and 1:100 Laminin-(Biotechne 3400-010-02 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized with 0.1% (weight/volume) Triton X-100 (Merck, Cat. no. X100) in PBS and the nuclei were stained with the DNA dye Hoechst 33342 (1 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were lysed resuspending with BugBuster and Lysonase according to the manufacturer’s protocol (Merck). Lysed cells were fractionated by centrifugation at 16000 g for 20 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... We then stimulated the cells for 4 h with 100 ng/ml PMA (524400; Merck) and 1 µg/ml ionomycin (407952 ...
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bioRxiv - Cancer Biology 2024Quote: Transwell migration assay was performed using the colorimetric QCM Chemotaxis Cell Migration Assay (ECM508, Merck), in 24 well-plate format with 8µm pore size ...
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bioRxiv - Microbiology 2024Quote: ... Any detached HBEC-5i cells were removed with Biocoll separation solution (Merck Biochrom, Berlin, Germany). The parasites were then grown under standard conditions to a parasitemia of 5-10% ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and stained using γH2AXSer139 –FITC 1:250 (16-202A, Merck Millipore). Cells were analyzed with BD Accuri C6 flow-cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... cells were treated with 100 nM Bafilomycin A1 or 1 µM Rapamycin (Merck, #553211-500UG) 24 h before harvesting ...
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bioRxiv - Immunology 2024Quote: ... The SH-SY5Y cell line was cultured in the minimum essential medium Eagle (EMEM, Merck) and F-12 nutrient mix (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were stimulated 8 h after transfection with TNF-α (Merck, catalog no. H8916-10UG), IL-6 (Prepotech ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed twice with PBS and detached by treating with 1 ml AccutaseTM (Merck) for 30 min at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: Cultured cells for 24 hours of incubation were fixed with 4% paraformaldehyde (PFA) (#104005, Merck) in Hank’s balanced salt solution containing 1 mM Ca2+ and Mg2+ ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were transfected with pcDNA3.1 and pEFl vectors using GeneJuice transfection regent (Merck Millipore, #70967-3) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... DNA and RNA were removed from the cell lysate using benzonase (1 U/ml, Merck Millipore) for 10 min on room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells (5 × 105) were mixed in 2ml viral supernatant supplemented with 8μg/ml polybrene (Merck Millipore) and further incubated at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were spun down again and stained using 0.25 μg α-H3S10p (Merck Millipore 04-817) in 1 % Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: A549 cells were treated with 2 ng/ml TGF-β1 (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 2 days in DMEM with 10% FBS on non-treated culture dish surfaces (31 ...
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bioRxiv - Cell Biology 2022Quote: ... according to the manufacturer’s protocol and analyzed using Muse Cell Analyser (Sigma-Aldrich/Merck, Poznan, Poland). Obtained data were used to determine PDT with an algorithm published by Heuer et al.[25] and Cell Calculator [26].
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bioRxiv - Cell Biology 2020Quote: ... Endothelial cells were isolated by administration of 0.1% collagenase D solution from Clostridium histolyticum (Merck KGaA) into the vein for 20 minutes at 37°C and subsequently cultured in endothelial cell growth medium purchased from PromoCell supplemented with 1% penicillin/streptomycin and used up to passage 4 for all indicated experiments.
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bioRxiv - Biophysics 2019Quote: ... the streptavidin tetramer mixture was concentrated in a stirred ultrafiltration cell (10 kDa cut-off, Merck). Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Microbiology 2021Quote: Blocks of cells or tissue were incubated overnight in 2.3M sucrose at 4°C (Merck, K17687153) in 0.1M phosphate buffer ...
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bioRxiv - Microbiology 2021Quote: ... cells and tissue were washed 3 times with phosphate buffered saline (PBS) + 0.02M glycine (Merck, K27662101) to remove fixative ...
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bioRxiv - Neuroscience 2020Quote: ROS levels of cells were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells pretreated with TC-HT or HT at 42.5°C high temperature setting for 8 cycles or 2 h ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were detached in a similar manner: 4 mg/mL lidocaine hydrochloride (L5647; Merck KGaA) were solved in Versene Solution freshly for each detachment ...
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bioRxiv - Neuroscience 2021Quote: ... covered with mounting medium conjugated with a nuclear cell body marker (Fluoroshield TM with DAPI, Merck) and protected using standard coverglass ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Merck), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were stained using ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) according to manufacturers instructions.
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bioRxiv - Synthetic Biology 2021Quote: ... the medium was removed and cells were fixed for 10 min in 4% formaldehyde solution (Merck). The fixation solution was removed and the cells were washed twice with nuclease-free H2O (nfH2O) ...