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Citations for Lucigen :
151 - 200 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: 2 μg of total RNA were treated with 4U of RNase R (Lucigen) at 37°C for 5 or 10 minutes in case of downregulated and at 37°C for 30 minutes for upregulated circRNAs followed by 20 minutes at 65°C ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Microbiology 2024Quote: ... was added to 0.3% final concentration and Plasmid-Safe ATP-dependent DNase (Epicentre E3105K) was added to 20 U/ml final concentration to digest any free unpackaged DNA ...
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bioRxiv - Microbiology 2024Quote: ... and rRNA was removed from 1 mg of total RNA with Ribo-Zero Magnetic Gold Kit (Epicentre Biotechnologies, Madison, WI, USA). To construct the RNA-Seq library ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was isolated (QuickExtract, Cat #QE0905T, Lucigen) and the gRNA-targeted site was amplified by PCR (primers listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... 20,000 U of Ready-Lyse lysozyme (Epicentre, Madison, WI, USA), and 40 U of SUPERase-In RNase inhibitor for 5 min at room temperature to extract total RNA ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA extractions were done using Epicenter MasterPure Yeast RNA Purification Kit (Lucigen, Middleton, WI). The RNA samples were treated with DNase I to remove all traces of DNA using TURBO DNA-free™ Kit (Invitrogen™ ...
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bioRxiv - Microbiology 2024Quote: ... Baseline ZERO DNAse (Lucigen), Benzonase (Sigma-aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Glu563 variant plasmid DNA was ordered from GenScript (Hong Kong) and transformed into C41 (DE3) cells (Lucigen), before overexpression and purification in a similar fashion to wildtype ...
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bioRxiv - Immunology 2024Quote: ... QuickExtract DNA Extraction Solution 1.0 (Epicentre) was used to generate bulk gDNA from dissociated cells to validate editing efficiency before clonal selection ...
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bioRxiv - Microbiology 2024Quote: ... MPC protein precipitation reagent (Lucigen) was added ...
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bioRxiv - Genomics 2024Quote: ... or the NxSeq® AmpFREE Low DNA Library Kit (Lucigen; # 14000-2). In total ...
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bioRxiv - Genomics 2024Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... a fraction of the cells was used for genomic DNA extraction using QuickExtract DNA Solution (Lucigen). PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of Hybridase Thermostable RNase H (Lucigen) was added to each tube and incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 500mL TES (10mM Tris-HCl, pH 7.5, 1mM EDTA, 100mM NaCl) and incubated with 35,000U of Ready-Lyse (Lucigen) for 10min at room temperature ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Disruption of both alleles of the target locus and integration of the RT were confirmed by PCR after DNA extraction (MasterPure Yeast DNA Purification Kit, Lucigen). The SAT1 gene was removed with the flipper system ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA extracted (QuickExtract™ DNA Extraction Solution, Lucigen, cat. QE09050) and the remainder restimulated with fresh medium and cultured for an additional 14 days (Jones et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA from islets and βTC6 cells were treated with RNase R (Epicentre), followed by RT-qPCR analysis of linear and circRNAs as mentioned previously 18 ...
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bioRxiv - Molecular Biology 2024Quote: ... total DNA and total RNA were extracted from one million cells of the porcine hepatocyte-enriched fraction using MasterPure DNA Purification Kit (Epicentre) and RNAzol RT (Molecular Research Center ...
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bioRxiv - Microbiology 2024Quote: Transposon mutagenesis was performed by electroporation using the EZ-Tn5
Tnp Transposome kit (Lucigen). Briefly ... -
bioRxiv - Microbiology 2024Quote: ... The parasite gDNA was extracted from the infected mosquito midguts 14 days post-infection (Barcode output) by boiling the midguts in Quick Extract DNA Extraction Solution (Epicentre). PlasmoGEM barcodes were amplified by PCR in using the extracted DNA samples and the primers 91_Illumina and 97_Illumina (Table S6) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen). The cells were rescued with 1 ml of recovery medium for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... pCML375 was constructed by inserting this fragment into the EZ-Tn5-carrying plasmid pMOD
(Lucigen). The transposon DNA was purified by digesting pCML375 using PvuII-HF (NEB) ... -
bioRxiv - Molecular Biology 2024Quote: ... by annealing locus-specific oligonucleotides to a common 5’ universal oligonucleotide and performing in vitro RNA transcription (AmpliScribe T7-Flash Kit, Lucigen, ASF3507)(Gagnon et al ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 1 × 107 cells in exponential growth phase using the Masterpure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cloni classic electrocompetent cells (Lucigen, 60117-1). Plasmid DNA was isolated and representation of the pool was verified by Illumina sequencing.
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bioRxiv - Biochemistry 2024Quote: ... coli strain MC1061 (Lucigen). Protein overexpression for purification was carried out in a derivative of E ...
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bioRxiv - Biochemistry 2024Quote: ... and 5’ polyphosphatase (Lucigen), using 1 μL of enzyme per 5 μg of uncapped RNA ...
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bioRxiv - Biochemistry 2024Quote: ... 5’ polyphosphatase buffer (Lucigen) and 5’ polyphosphatase (Lucigen) ...
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bioRxiv - Biochemistry 2024Quote: ∼4 pmol of the purified ligated product obtained from an overnight ligation reaction between CS3 and PPP-Lig was digested with 20 U of RNase R (Lucigen) in a 10 μL reaction in the presence of 1X RNase R buffer at 50 °C for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... Then a two-step enzymatic digestion using 5’ polyphosphatase (Lucigen) and Xrn-1 (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... was transformed into C41-competent cells (Lucigen Corporation, WI, USA). After 16 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: DNA template for the SreA aptamer domain was generated by PCR amplification of the HH SreA pUC57 plasmid with EconoTaq PLUS 2x Master Mix (Lucigen) using primers that are complementary to the 5′ and 3′ ends of P1 (Table S3) ...
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bioRxiv - Molecular Biology 2024Quote: ... or QuickExtract DNA extraction solution (Lucigen, #QE0905T) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 50 U of RiboGuard RNase Inhibitor (Lucigen), 1x TURBO reaction buffer and 5-10 µg of total RNA ...
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bioRxiv - Genomics 2024Quote: ... 1 μL cDNA (600 pg) and 1 μL of tagmentation buffer (TNP92110, Lucigen) were added to 8 μL Tn5 mix ...
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bioRxiv - Genomics 2024Quote: ... first-strand cDNAs were synthesized from 1 µg of DNase-treated RNA using the MMLV Reverse Transcriptase Synthesis Kit (Lucigen) protocol with the Oligo(dT)21 primer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and transformed into Endura electrocompetent cells (Lucigen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 1 μL of 10X stop buffer (TNP92110, Lucigen) was then added to the mixture to quench tagmentation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells in one set were harvested to extract the genomic DNA with 50 μL of QuickExtract DNA Extraction Solution per well (Epicentre), which were subsequently used for PCR screening using Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was performed with Failsafe PCR 2X Mastermix (Lucigen, FSP995H), Failsafe Enzyme (Lucigen ...
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bioRxiv - Genetics 2024Quote: ... and aliquots mock-treated or treated with T4-endonuclease V (T4endoV, Epicentre). DNA was electrophoresed in 1.3% alkaline agarose gels ...
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bioRxiv - Plant Biology 2024Quote: Gel-purified cDNAs were circularised using CircLigase ssDNA Ligase (Lucigen) and 5 M betaine (VWR ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted from surviving cells (Lucigen QuickExtract or Qiagen DNeasy DNA blood and tissue kit ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted using Quick Extract DNA extraction solution (Epicentre Catalog no. QE09050). Colonies were screened by PCR and sequenced using primers flanking each SNP (Primer sequences are provided in Table S6A) ...
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bioRxiv - Genetics 2024Quote: ... which contains a mix of T5 exonuclease (T5E4111K 1000U from Epicentre Biotech-Ecogen), Phusion polymerase (F530s 100U from VITRO and a Taq DNA ligase (Protein Technologies Unit CRG ...
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bioRxiv - Genomics 2024Quote: ... Ligated products were pooled and concentrated using the DNA Clean and Concentrator kit before being electroporated into Endura chemically competent cells (Lucigen). The 94-member library was purified using the Midi Plus kit (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was isolated from the thawed cell pellets using the MasterPure Yeast DNA Purification kit (Lucigen MPY80200). The DNA was digested with XhoI and NgoMIV and fractionated on a 0.6% agarose gel ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X RNase H buffer (Epicentre) and 6 µL of nuclease-free water were added and the samples were incubated at 45°C for 30 min ...