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Citations for Lucigen :
151 - 200 of 2386 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... with RiboGuard RNase inhibitor (Lucigen, Cat # E0126-40D7) and oligo-dT primers (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli DH5α or CopyCutter EPI400 (Lucigen) was used for cloning and grown in Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Genetics 2023Quote: ... DNA were extracted using MasterPure yeast DNA purification kit (Epicentre, MPY80200). ChEC DNA was subjected to size selection using the Pippin Prep (SageScience ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen) were transformed with 2.5 µl of the eluate each ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were sorted in 5 μl of QuickExtract DNA Extraction Kit (Epicentre, USA) in 96-well format.
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bioRxiv - Neuroscience 2023Quote: 1 µg of BH RNAs was incubated with 1 U/µg RNase R (Lucigen RNR07250) at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was done by using the MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) to gain high quality and quantity DNA for metagenomic sequencing ...
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... and residual linear DNA was degraded by Plasmid-Safe ATP-dependent DNase (Lucigen). In vitro cleavage reactions were performed with 250 ng of Plasmid-Safe-treated circularized DNA ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis and strand-specific library generation was carried out using the Script-Seq V2 Kit (Epicentre) and the FailSafe PCR enzyme mix ...
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bioRxiv - Genomics 2023Quote: ... with the following modifications as previously published37: Swabs were placed in tubes containing 350 ul Tissue and Cell Lysis solution (Lucigen), 1.5 mg/ml lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... then resuspend in 1mL resuspension buffer (20 mM Tris-HCl pH 7.5, 300 mM NaCl, 12500 U Ready-lyse (Epicentre technologies), and one tablet of EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage selections were amplified by adding them to 1.0 ml TG1 cells (Lucigen) grown to mid-log phase (OD ∼ 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified cDNA was then circularized using CircLigaseII ssDNA ligase (Epicentre) for 1[h at 60°C and was subsequently PCR amplified using the primers 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC-3′ and 5′-CAAGCAGAAGACGGCATACGAGATJJJJJJGTGACTGGAGTTCAGACGTGTG-3′(where Js indicates the 6-mer index sequence for Illumina sequencing).
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bioRxiv - Microbiology 2023Quote: ... ribosomal RNA was removed using the Ribo-Zero rRNA removal kit for Gram-negative bacteria (Epicentre/Illumina), and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina) (76) ...
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bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen #60242-1) using a Gene Pulser Xcell (Bio-Rad #1652662) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen, Middelton, WI, USA). 50 ng of genomic DNA were used to amplify the region that spans the cutting site of each gRNA using KAPA2G Fast ReadyMix (Kapa Biosystem ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using the MasterPure Yeast DNA purification kit (Epicentre, MPY80200), and concentrations were measured by Qubit ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of RNase inhibitor (Lucigen) and 450 μl of IP buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... and excess unligated linker was removed using 10 U/µl 5’ deadenylase/RecJ exonuclease (Epicentre, RJ411250), followed by pooling and purification of ligated footprints using an Oligo Clean and Concentrator column (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA (gDNA) was extracted by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Microbiology 2023Quote: ... Samples were collected using sterile Catch-All™ Specimen Collection Swabs (Epicentre Biotechnologies, Madison, WI), and placed in MoBio bead tubes with buffer (MoBio PowerSoil™ kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Single clones were expanded and genotyped for the desired knock-in by PCR on genomic DNA extracted with Quickextract solution (Lucigen). Clones showing biallelic knock-ins were expanded and frozen in LN2 at early passages ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using to MasterPure Yeast RNA Purification kit protocol (Epicentre) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturer’s instructions (Lucigen). In all cases ...
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bioRxiv - Plant Biology 2023Quote: ... coli [C41 pLysS (Lucigen, USA)] by cloning their coding sequences in pET28a(+ ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5% SDS) and purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, WI, USA). RNA was prepared by TRizol according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli 10G is a highly competent DH10B derivative [39] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C43 (DE3) cells (Lucigen) while VLCADWT (res ...
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bioRxiv - Genomics 2023Quote: ... The MDA reactions were prepared using RepliPHI Phi29 DNA polymerase and Reagent set (Epicentre, Illumina, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 250 pmol of Qβ-RNA were treated with 60 U of RNA 5’-polyphosphatase (Epicentre) in 1 x polyphosphatase reaction buffer at 37 °C for 70 min ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA were extracted using QuickExtract DNA Extraction Solution (Epicentre). PCR amplification products of the mutation site were used in restriction fragment length polymorphism assays with the AluI restriction enzyme (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... cloni 10G (Lucigen) was used for library construction ...