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Citations for Lucigen :
101 - 150 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Biochemistry 2024Quote: ... washed with water and total cellular RNA was isolated using a MasterPure Yeast RNA Purification Kit (Lucigen) according to the vendor’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... encoding residues 21 to 344 which lacks the N-terminal mitochondrial-targeting sequence) was cloned into pSol vector (Lucigen) to generate the pSol-His8-SUMO-MGME1 plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... and circularized with circLigase2 (Epicentre). The cDNA templates were amplified using Phusion polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... cloni 10G cells were procured from Lucigen. All the primers used in this study were obtained from Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA isolation was performed using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to manufacturer’s instructions and total gDNA was quantified using a nano-drop (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... and expressed in BL-21 (DE3) cells (Lucigen, Middleton, WI). Bacterial cultures were grown overnight at 37°C in 2x YT (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... and an excess of Expresso Sumo Protease (Lucigen). Following a 2-hours incubation at room temperature with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments sized between 30 and 40 kb were isolated as previously described (Tasse et al., 2010) and cloned into pEPIFOS-5 fosmids (Epicentre Technologies). EPI100 E ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting cDNA products were circularized with CircLigase ssDNA ligase (Lucigen, USA). Illumina sequencing adaptors were introduced by PCR and the final library sequenced at the JHU Genetic Resources Core Facility on a HiSeq2500 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic mapping to mouse GRCm38 and RepeatMasker analysis was performed by Lucigen. Read quality was evaluated by Python Bio.SeqIO56 and FastQC57 ...
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bioRxiv - Cancer Biology 2024Quote: ... treatment was sequenced by Lucigen using the Mini-Y Adapter Library kit specialized for small DNA strands ...
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bioRxiv - Cancer Biology 2024Quote: ... and gDNA was extracted by using QuickExtract DNA Extraction solution (Epicentre) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted cDNA was circularized with CircLigase II ssDNA ligase (Lucigen, CL9021K) for 1 hr at 60°C ...
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bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
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bioRxiv - Cell Biology 2024Quote: ... We treated 10 μg of RNA with RNase I (Epicentre) for 45 min at 25°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.75 mM biotinylated UTP (Biotin-16-UTP, Lucigen BU6105H) and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044 ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted using 50µL QuickExtract DNA Extraction Solution (Lucigen, Qiagen). DNA sequences surrounding the deletion site were amplified using the following primers ...
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bioRxiv - Bioengineering 2024Quote: ... 20ul of QuickExtract (Epicentre) buffer was added ...
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bioRxiv - Biochemistry 2024Quote: ... Genotypes of off-spring were identified by isolating DNA using QuickExtract buffer (Epicentre, #QE09050), subsequent amplification of the targeted area using primers G1 and G2 and employing the T7 endonuclease assay ...
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bioRxiv - Biochemistry 2024Quote: ... cloni® cells (Lucigen) and plated onto LB agar supplemented with 100 µg/mL kanamycin ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was extracted from frozen slices using QuickExtract buffer (Lucigen). The genomic region containing CRISPR-edits was PCR amplified from genomic DNA template using RedExtract PCR mix (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was prepared using Quick Extract (Lucigen) and column purified ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed with PBS and genomic DNA extracted with Quick Extract (Lucigen), PCR amplified in the human ApoE gene exon 4 containing the E4 target base (see below) ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated from ear clips using QuickExtract buffer (Lucigen) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated from ear clips using QuickExtract buffer (Lucigen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell colonies originating from a single clone were isolated in 12-well plates and genomic DNA was extracted using QuickExtract DNA Extraction Solution (Lucigen) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... Electrocompetent bacteria (Lucigen, #60242-1) were electroporated (10 µF ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μl QuickExtract DNA Extraction Solution (Lucigen) was used following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was extracted from hPSCs using Quick Extract DNA solution (Lucigen, QE09050). Gene targeting specific amplicon was amplified using one primer on the bGH poly A sequence of the insert and the other primer outside the right homology arm ...
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bioRxiv - Plant Biology 2024Quote: ... and 50 mM EDTA) and mixed with 10 µl Ready-Lyse lysozyme (Epicentre Biotechnologies, Madison, WI, USA) and 50 µl RNase (10 mg/mL) ...
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bioRxiv - Biochemistry 2024Quote: ... 1U/µl RiboGuard RNase Inhibitor (Lucigen; RG90925), 0.1 U/µl 5’-Phosphate-Dependent Exonuclease ...
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bioRxiv - Biophysics 2024Quote: ... cloni® 10G cells (Lucigen). All cultures contained 50 μg/ml kanamycin for bacterial selection ...
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bioRxiv - Bioengineering 2024Quote: ... DNA extraction from various hiPSC clones was carried out using the QuickExtract DNA Extract Solution (Epicentre), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cells transduced with a sgControl or a sgRNA targeting UXS1 or VCAN were collected and their genomic DNA extracted using QuickExtract DNA (Lucigen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... EconoTaq Plus Greeen (30033, Lucigen) was purchased ...
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bioRxiv - Cancer Biology 2024Quote: ... eukaryotic mRNA was enriched with Oligo (dT) beads and prokaryotic mRNA was enriched by removing rRNA with Ribo-ZerotM Magnetic Kit (Epicentre, Madison, WI, USA). The enriched mRNA fragments were then split into short fragments using lysates and reverse transcribed into a strand of cDNA using random primers ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted using QuickExtract (Epicentre) and genotyping PCRs were performed with MyTaq HS Red Mix (Bioline ...
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bioRxiv - Cell Biology 2024Quote: ... End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25°C for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: The mouse metabolism library (ref32) was a generous gift from Kivanc Birsoy and was amplified using Endura Electro-Competent cells (Lucigen). Lentivirus was produced for the screen library by the co-transfection of the screen library plasmid with the packaging plasmids psPAX2 and pMD2.G into 293T cells as described above ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification was carried out using Premix D mixture of the FailSafe™ PCR System (Epicentre, # FS99250). The following primers were used for this analysis ...
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bioRxiv - Biochemistry 2024Quote: ... was transformed into electrocompetent MC1061 cells (Lucigen) to maximize transformation efficiency.38 25 µL of cells were pre-chilled on ice for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... DNA extraction was done using QuickExtract DNA Extraction Solution (Epicentre, #E09050) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from these clones using the QuickExtract DNA extraction solution (Catalog No. QE09050, Lucigen) following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 10 μL RNAse mix (2 μL thermostable RNAseH (Epicentre), 8 μL RNAse buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 30mM MgCl2 buffer was added to each reaction plus 2.5µg of Rnase A (EpiCentre) for 1hr at 37°C ...
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bioRxiv - Immunology 2024Quote: ... gDNA isolation was performed from a portion of the clones via QuickExtract DNA Extraction Solution 1.0 (Epicentre). Genotyping primers were created by designing flanks around the mutation site ...