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Citations for Thermo Fisher :
4901 - 4950 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed by using a gene specific primer (1 μM final concentration) located near the 3’ end of the FLEXI intron with 100 U Maxima H reverse transcriptase (ThermoFisher) in the manufacturer’s buffer and incubated at 25°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... we prepared for each sample and IgG control: 120□μl of Dynabeads M-280 Sheep anti-mouse IgG (Thermo Fisher) or 40□μl of Dynabeads protein G (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed 3 times for 2 min each in 0.01 M PBS with 0.2% Triton X-100 and were incubated in Alexa Fluor secondary antibodies (Thermo Fisher Scientific) (Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then lysed by glass bead disruption and incubated with IgG-coupled magnetic beads (Dynabeads M-270 Epoxy from Invitrogen) with slow tilt rotation for 1 h at 4°C in Buffer 30 (20 mM HEPES (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellet sections were imaged using a Talos L120C microscope operated at 120 kV with a Ceta-16 M (4,096 × 4,096) camera (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... and 17 µL of 1x RNA Sequencing Buffer (20 mM sodium citrate pH 5, 1 mM EDTA, 7 M urea) (Ambion). The mixture was split across three tubes (9/8/8 µL) ...
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bioRxiv - Cell Biology 2024Quote: ... Freshly harvested primary monocytes were plated at a density of 20-25 M per 10 cm plate in macrophage culture media containing: RPMI (11875119, ThermoFisher), 10% FBS (26140079 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with 0.1 M phosphate buffer and incubated with Alexa Fluor secondary antibodies (Fisher Scientific, dilutions 1:1000) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... CTG Hairpin-Biotin and GC Clamp-Biotin were bound to Streptavidin Magnetic Beads (Dynabeads® M-280 Streptavidin, Life Technologies) with a 50 nM final concentration of biotinylated antigen for the first round and 10 nM final concentration of biotinylated antigen for the second and third rounds.
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bioRxiv - Biophysics 2020Quote: ... Sections were then incubated with an anti-rat Alexa Fluor-488 secondary antibody (1:300 dilution; Catalog #A-11006, Invitrogen) for 1 hour ...
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bioRxiv - Biophysics 2020Quote: Ki-67 immunostaining was performed using a rat anti-Ki-67 antibody (1:400 dilution; Catalog #14-5698-82, Invitrogen). All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024 ...
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bioRxiv - Cell Biology 2020Quote: Isolation of mouse lung endothelial cells was performed according to the following protocol: Day 1-Incubate sheep anti-rat IgG Dynabeads (Invitrogen) overnight with PECAM (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Abberior), anti-rat STAR RED (1:100, Abberior) and anti-guinea pig Alexa Fluor-488 (1:1000, Thermo Fisher Scientific) and DAPI (1 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 2 hrs with Alexa Fluor 555-conjugated goat anti-rat antibody and Alexa Fluor 488-conjugated goat anti-mouse antibody (both 1:200; Invitrogen). Fiber images were acquired by fluorescence microscopy and analyzed manually using Image J ...
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bioRxiv - Cell Biology 2020Quote: ... BM cells from non-doxycycline treated mice were isolated and lineage depleted using Sheep Anti-Rat IgG magnetic Dynabeads (Invitrogen). The lineage depleted cells were heat activated for an hour at 37°C prior to placing them on the 5μm pore transwell insert ...
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bioRxiv - Immunology 2021Quote: ... slides were washed twice with PBS and stained with secondary antibodies (1:400; streptavidin conjugated goat anti-rat IgG-Alexa fluor 488 from Invitrogen) for 2-3 hr at room temperature in a humidified chamber followed by incubation in prolong gold antifade reagent with DAPI (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-647 phalloidin (Invitrogen) if needed ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:500), Alexa 555-labeled goat anti-rat IgG (A21434, 1:500), Alexa 555-labeled streptavidin (S32355, 1:500) from Invitrogen; horseradish peroxidase-conjugated goat anti-rabbit IgG (1706565 ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... Coverslips were subsequently washed in quadruplicate (5 min each) with PBS and diluted secondary antibody (1:500 goat anti-rat IgG Alexa Fluor 488 conjugate, Invitrogen) was applied as above ...
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bioRxiv - Immunology 2021Quote: ... mice were woken up to allow any fecal matter to evacuate and then anesthetized again to introduce 100μL of Cy-5-labelled glucose or Cy-5 secondary goat anti-rat antibody (0.1mM diluted in PBS, ThermoFisher A10525) into the colon via a gavage needle enema ...
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bioRxiv - Bioengineering 2020Quote: ... the slides were washed extensively with PBS and incubated with secondary antibodies (Alexa Fluor 488 goat anti-rat IgG, Invitrogen, Carlsbad ...
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bioRxiv - Bioengineering 2020Quote: ... the slides were washed extensively with PBS and incubated with secondary antibodies (Alexa Fluor 488 goat anti-rat IgG, Invitrogen, Carlsbad, CA or Alexa Fluor 568 donkey anti-rabbit IgG, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... mice received an injection of rat anti-mouse tumor necrosis factor (TNF) antibody (2.5 mg/kg intraperitoneal, clone MP6-XT22, Thermo Fisher) or a control isotype antibody (IgG1 kappa ...
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bioRxiv - Cell Biology 2019Quote: ... Primary cultured cortical neurons were isolated from male and female embryonic day 18 Sprague-Dawley rats and maintained in Neurobasal medium (Gibco) supplemented with 2mM glutamine (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were stainied with secondary antibodies in 0.3% triton-X100/PBS for 2 hours at room temperature (anti-rat Alexa Fluor 568, Thermo Fisher A-11077 ...
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bioRxiv - Neuroscience 2019Quote: ... The hCMEC/D3 96-well plates had been pre-coated with 0.1mg/ml rat tail collagen type1 (Gibco cat#A1048301).
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bioRxiv - Developmental Biology 2019Quote: Cortical hemispheres from neonatal rat pups (P1-P3) were isolated and dissected in ice-cold DMEM containing penicillin-streptomycin (Gibco). Care was taken to remove meninges and white matter ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS containing 0.3% Triton X-100 (PBS-Tx) overnight at 4◦C followed by incubation with goat-anti-rat AlexaFluor647 (1:500; ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Sprague-Dawley rat embryos (Hilltop Labs Inc., Pittsburgh, PA) and then plated and maintained in neuronal medium consisting of Neurobasal medium (Invitrogen) supplemented with 1% penicillin-streptomycin with 2 mM glutamine (Invitrogen) ...
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A Comprehensive Integrated Anatomical And Molecular Atlas Of Rodent Intrinsic Cardiac Nervous SystembioRxiv - Neuroscience 2019Quote: ... Microarrays were used to perform transcriptomic assays on all samples by the Thomas Jefferson University Cancer Genomics Core using the Clariom S Assay for rat from Affymetrix.
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bioRxiv - Bioengineering 2020Quote: ... Brain sections were stained in a 1:220 dilution of goat anti-rat IgG + 500 U RNase inhibitor (Life Technologies). After 1-hour exposure ...
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bioRxiv - Genomics 2019Quote: Dispersed rat islet cells or FACS-sorted β-cells were transfected with a pool of 4 siRNAs directed against rat Scrt1 or a negative control (On-Target plus 081299-02 and 001810-10 respectively, Dharmacon) using Lipofectamine RNAiMAX (Thermofisher). INS 832/13 cells were co-transfected with pGL3 promoter and psicheck plasmids (Promega ...
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bioRxiv - Biophysics 2019Quote: ... cRNA of TRPV1 transcribed from the rat TRPV1 clone in the pGEMSH vector using the mMessage mMachine kit (Thermo Fisher) was injected into Xenopus laevis oocytes ...
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bioRxiv - Neuroscience 2020Quote: ... hippocampal tissue from E19 Sprague Dawley albino rat embryos was harvested on ice in 4°C Hibernate E (Life Technologies) and digested with 20 units/mL papain (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... the free-floating sections were incubated for 2 h in staining solution together with anti-rat Alexa 594 and anti-rabbit Alexa 647 (1:400, ThermoFisher). Slices were washed 3 times with PBS and mounted using Dako Fluorescent mounting medium (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... EDC (20 mM) was added to the solution which was combined with rat tail collagen I (Gibco, 3 mg/mL) at a 70:30 gelatin ...
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bioRxiv - Biochemistry 2020Quote: ... slides were rinsed with PBS and incubated for 1h with secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen) and DAPI (4083 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor goat-anti rabbit 488 nm, goat anti-rat 594 nm, Invitrogen) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used in this study: rat anti-mCherry (Life Technologies cat#M11217, 1:500–1:1000), chicken anti-GFP (Aves Labs cat#GFP-1020 ...
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bioRxiv - Developmental Biology 2020Quote: ... The following secondary antibodies from Thermo Fisher Scientific were incubated at room temperature for one hour: Donkey anti-rat Alexa488 (1:100, Thermo Fisher, Cat # A-21208), Donkey anti-rat Alexa594 (1:100 ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496, 1:1,000; chicken anti-GFP, Life Technologies A10262 ...
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bioRxiv - Neuroscience 2020Quote: ... and detection carried out with fluorescent-labeled secondary antibodies (goat anti-rat, 1:800, Alexa Fluor 488, A-101650, Invitrogen). For the detection of mCherry endogenous fluorescent protein animals were anesthetized with Avertin ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217 ...
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bioRxiv - Immunology 2021Quote: ... 1:100) followed by anti-rat secondary antibody conjugated with Alexa Fluor 674 (Thermo Fisher, catalog no. A21247, 1:200), and stained for subcapsular sinus macrophages using anti-sialoadhesin (CD169 ...
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bioRxiv - Microbiology 2021Quote: ... and rat bladder carcinoma cell line 804G were cultured in Minimal Essential Media (MEM; Gibco, Grand Island, NY, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217 ...