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Citations for Thermo Fisher :
4951 - 5000 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was used at a concentration of 1:100 followed by treatment with 1:1000 goat anti-rat IgG (H+L) cross-adsorbed secondary Alexa Fluor 594 antibody (ThermoFisher) to detect PARP ...
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bioRxiv - Microbiology 2021Quote: ... NRK-49F normal rat kidney fibroblasts (ATCC CRL-1570) were seeded in Nunc Square BioAssay Dishes (Thermo Scientific, ref. 166508) with 50 mL Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... slides were treated with appropriate secondary antibodies (1:1000 goat anti-rat/rabbit Invitrogen, 1:1000 goat anti-chicken, Abcam) following PBS rinsing ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed with 0.1% PBST (3 x 10 min) followed by incubation with fluorophore-conjugated secondary antibodies: donkey anti-Rat Dylight 488 or 647 (1:500, Invitrogen) and donkey anti-Goat Alexa 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with both donkey anti-rat Cy3 (1:800; Jackson Immuno Research) and Alexa Fluor-488 donkey anti-rabbit (1:500; ThermoFisher) in 1.5% NDS (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... and the secondary antibody (Alexa488 conjugated anti-rat or anti-rabbit IgG in 1/100 dilution; Thermo Fisher Scientific, US) were suspended in Can get signal solution (Toyobo ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary Abs (raised in goat) were used: anti-rat or anti-rabbit IgG-Alexa Fluor (AF) 488 or 594 (ThermoFisher), biotin-labelled anti-rat IgG (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... Purity was verified via SDS-PAGE and circular dichroism by comparison of a commercially available rat tail collagen solution (Invitrogen) (Supplemental Figure 1).
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bioRxiv - Cell Biology 2021Quote: ... 15 mM HEPES DMEM/F12 no phenol red) were seeded onto plates coated with 50 μg/ml collagen type I from rat tail (ThermoFisher) for 1 to 2h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... To construct standard curve for genes of interest total RNA was extracted from homogenized head of 15/16 days rat embryo (Fischer 344) by TRIzol reagent (Invitrogen). 5 μg total RNA was treated with DNase (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... were evenly coated with 75 µL of a 1 mg/mL collagen solution composed of 3 mg/mL rat tail collagen (Gibco), serum free media ...
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bioRxiv - Cell Biology 2021Quote: ... used in this study were anti-Nanog (monoclonal rat Ab, clone eBioMLC-51; 1:500, cat. num. 14-5761-80, ThermoFisher), anti-Rex1 (rabbit polyclonal Ab ...
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bioRxiv - Bioengineering 2022Quote: Total miRNA from fetal rat lung was extracted using the mirVana miRNA Isolation Kit (Cat# AM1560, Ambion, Austin, TX, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies (goat anti-mouse Alexa Fluor 488, goat anti-rat Alexa Fluor 555, goat anti-rabbit Alexa Fluor 647; Invitrogen) were all prepared in 3% NGS in 1XPBS at a dilution of 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated with Alexa Fluor® 594 anti-rabbit and Alexa Fluor® 488 anti-rat secondary antibodies (ThermoFisher) at 1:500 in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... For IFA secondary antibodies were Alexa Fluor 594 or Alexa Fluor 488-conjugated goat anti-rabbit and goat anti-rat (Invitrogen), both used at 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated for 2 hrs in 40 µL of secondary antibody (1:1,000 anti-rat Alexa Fluor 488, Life Technologies) in 3% BSA in PBS ...
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bioRxiv - Biophysics 2022Quote: The full-length Rph3A (1–681) gene was amplified from rat brain cDNA library and cloned into the pEXP5-NT/TOPO vector (Invitrogen). The Rph3A fragments 1–281 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were rinsed twice in PBS before adding Alexa Fluor 594 conjugated goat anti-rat secondary antibody (ThermoFisher Scientific, A48264) diluted 1:500 in blocking buffer and incubated for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Afterwards the preparations were washed with PBS 8 x 30 min and then incubated with secondary antibodies (donkey anti-chicken Cy2, 1:400, Dianova, cat# 703-505-155; donkey anti-rat Cy3, 1:400, Invitrogen, A-21209 ...
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bioRxiv - Plant Biology 2021Quote: ... The immunolabeling protocol was similar to that described above except that Alexa Fluor® 594 goat anti-rat (Molecular Probes) was used as the secondary antibody/fluorchrome combination ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed twice with PBS and incubated with fluorescent secondary antibodies (Anti-Rat Goat Alexa Fluor 488 [A-11070], Anti-Mouse Goat Alexa Fluor 647, Thermofisher) in PBSA-T for one hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor 647–conjugated secondary antibodies (anti-rabbit, A21244, 1834794; anti-rat, A21247, 1719171; anti-mouse, A21235, 1868116; and anti-hamster, A21451, 1572558, Invitrogen), biotinylated secondary antibodies (anti-rat ...
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bioRxiv - Immunology 2020Quote: ... Coverslips were extensively washed with PBS + 0.1% Tween-20 and incubated with Alexa 564 conjugated rat IgG antibody and Alexa 488 conjugated rabbit IgG antibody (Invitrogen) in blocking buffer at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with goat anti-rat IgG conjugated to Alexa-Fluor 488 (1:500, Invitrogen Life Technologies, cat# A11055) and donkey anti-rabbit IgG conjugated to Alexa-Fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with goat anti-rat IgG conjugated to Alexa-Fluor 488 (1:500, Invitrogen Life Technologies, cat# A11055) and donkey anti-rabbit IgG conjugated to Alexa-Fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor secondary antibodies were diluted to 1:500 in blocking solution and included: 488 goat anti-rat (Invitrogen #A21212), 488 goat anti-chicken (Invitrogen #A11039) ...
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bioRxiv - Microbiology 2022Quote: ... AlexaFluor 488 goat anti-rabbit IgG (1:500) and AlexaFluor 594 donkey anti-rat IgG (1:500) (Life Technologies, UK), as well as DAPI (1:3000 ...
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bioRxiv - Neuroscience 2019Quote: ... Ntrk2 (NM_012731.2) was amplified by PCR from rat cDNA and recombined into pDONR201 entry vector by BP clonase II reaction (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... dorsal root ganglion neurons were trimmed and isolated from postnatal 8-10 day SD rat spinal cords in HBSS (Invitrogen), and digested in 2.5 U/ml dispase II (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... Purity of the collagen was verified via SDS-PAGE and mass spectroscopy by comparison of a commercially available rat tail collagen solution (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 1h with a secondary antibody: Alexa-488nm donkey anti-rat or goat anti-mouse or goat anti-rabbit (Invitrogen) (dilution 1/400) ...
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bioRxiv - Developmental Biology 2020Quote: ... Each slice was embedded into 10 μl of rat type I collagen (Roche Diagnosis; diluted at 80% with 1X MEM (GIBCO), 1X GlutaMax (GIBCO ...
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bioRxiv - Cancer Biology 2020Quote: ... The sections stained for hNuclei were co-incubated with an eFluor 570-labelled rat monoclonal anti Ki-67 antibody (SolA15; ThermoFisher). After washing ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by Alexa Flor 488-conjugated donkey anti-mouse IgG or Alexa Flor 546-conjugated goat anti-rat IgG (Invitrogen), respectively ...
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bioRxiv - Microbiology 2021Quote: ... This was followed by 3×washes with PBS then incubation with Alexa Fluor 488 goat anti-mouse or Alexa Fluor 594 goat anti-rat antibodies (1:1000; Invitrogen) in PBS containing 3% BSA with DAPI ...
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bioRxiv - Physiology 2021Quote: Total RNA was isolated from approximately 100 mg of rat liver using TRIzol® reagent (Invitrogen®, Carlsbad, CA, USA), including the digestion of contaminating DNA with amplification grade DNAse I (Invitrogen®) ...
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bioRxiv - Pathology 2020Quote: ... Department of Comparative Medicine on formalin-fixed paraffin embedded sections using the following primary antibodies: rat monoclonal anti F4/80 (Clone BM8, Invitrogen, Cat ...
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bioRxiv - Microbiology 2020Quote: ... the coverslips were incubated with Alexa Fluor 488–conjugated goat antibody to rat IgG (A-11006; Molecular Probes; 1:200), Alexa Fluor 594–conjugated goat antibody to rat IgG (A-11006 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to GP96 were immunoprecipitated with an antibody against Grp94 (Rat, mAb, Enzo) and Dynabeads Protein G (Thermo Scientific), then examined using mass spectrometry analysis ...
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bioRxiv - Microbiology 2021Quote: ... with the following changes: goat anti-rat and streptavidin Molecular Probes Alexa-Fluor-488 and 555 conjugated secondary mAbs (Invitrogen) were used at 4µg/ml ...
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bioRxiv - Immunology 2019Quote: ... then incubated for 90 min at 37°C with goat anti-mouse and Rat antibodies conjugated to AlexaFluor-488 or 568 and DAPI (Invitrogen). All microscopy was performed using Nikon Structured illumination microscopy (N-SIM ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were subsequently washed in multiple changes of PBS and incubated with fluorescent dye–conjugated secondary antibodies (1:1,000; mouse, rat, or rabbit; Alexa Fluor; Life Technologies) in gel block or MOM-containing gel block when appropriate ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 1.5 h with goat-anti-mouse Alexa 488 and goat-anti-rat Alexa 594(both 1:500, Life technologies) and mounted with Vectashield medium ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Donkey anti-Mouse Alexa Fluor 568 (1:500, no. A10037), and Donkey anti-Rat Alexa Fluor 594 (1:500, no. A21209)–conjugated secondary antibodies were from ThermoFisher Scientific.