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Citations for Thermo Fisher :
4851 - 4900 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: We lysed cells using a Dounce homogenizer 20-30 times in 400 μL PBS (Wisent) containing 0.01 M dithiothreitol (DTT, ThermoFisher Scientific) and 1X protease inhibitor cocktail (100X stock ...
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bioRxiv - Molecular Biology 2023Quote: ... peptides were separated on a 20-cm self-packed column with 75µm inner diameter filled with ReproSil-Pur 120 C18-AQ (Dr.Maisch GmbH) mounted to an EASY HPLC 1000 (Thermo Fisher) and sprayed online into an Q Exactive Plus mass spectrometer (Thermo Fisher) ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: HCT116-shYAP/siTAZ cells were created by transfecting 100nM of TAZ siRNA (Dharmacon siGENOME SMARTpool #M-016083-00-0005) into HCT116-shYAP cells using Lipofectamine 2000 (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 µM Cas9 protein (IDT) and 2 µM of each annealed cr/tracr RNA were combined in Opti-MEM media (Gibco 11058-021 ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μm tissue sections were prepared on Leica VT1200S Vibratome and were mounted on slides with ProLong Glass Antifade Mountant (ThermoFisher). All images were acquired using STELLARIS 5 confocal microscope system (Leica) ...
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bioRxiv - Biophysics 2023Quote: ... PLL with 0.1 mg/L were incubated on the surface for 30 minutes and washed with 0.1 M HEPES (Gibco ™, USA) buffer ...
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bioRxiv - Neuroscience 2023Quote: ... were prepared as 10−3 M aliquots (PROC and CCAP in distilled water, RPCH in dimethyl sulfoxide: DMSO, Fisher Scientific) and stored at −20°C until use ...
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bioRxiv - Systems Biology 2023Quote: ... The bottom tips were packed sequentially with three plugs of C18 membrane (3 M Empore) and mixed-mode ion exchange beads (SCX:SAX = 1:1, Applied Biosystems). The quantity of C18 membrane and mixed-mode ion exchange beads was adjusted based on the protein amount.
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bioRxiv - Microbiology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed with the RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific) using random primers ...
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bioRxiv - Cell Biology 2023Quote: ... mice were perfused transcardially with 10 ml of RPMI with 200 ul of M-450 Dynabeads (Thermo Fisher Scientific, 14013). Kidneys were immediately extracted and minced using a razor blade ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... 740984.50) and cDNA was synthesized from 1 µg total RNA using the M-MLV Reverse Transcriptase (Thermo Fisher Scientific, 28025013). cDNA was amplified using LightCycler 480 SYBR Green I Master mix (Roche Diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody was cross-linked by washing twice with 0.2 M triethanolamine (pH=8.2) and resuspending in fresh DMP solution (20 mM dimethyl pimelimidate dihydrochloride (Fisher Scientific, P08925G) in 0.2 M triethanolamine ...
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bioRxiv - Microbiology 2023Quote: ... 0.25 µM of each dNTP as well as 0.4 U Phusion hot start polymerase and the respective buffer from Thermo Scientific. For samples from 0 dpc ...
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bioRxiv - Molecular Biology 2023Quote: ... using biotinylated RNA oligonucleotides (LGC biosearch technologies, Risskov, Denmark, sequences in supplements) immobilized on Dynabeads M-280 streptavidin magnetic beads (Invitrogen) and incubated with HeLa cell nuclear extract ...
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bioRxiv - Microbiology 2023Quote: ... Samples were heated 5 min at 95 degrees and analysed on a 7 M urea 15% acrylamide gel further stained with SYBR Gold (ThermoFisher). As a positive control ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNAs were synthesized from 200 ng of total RNA by using oligo dT primer and M-MLV Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended with 100 μl 1% SDS in presence of a Halt protease inhibitor cocktail and 0.5 M EDTA solution (Thermo Fisher) and cells lysed mechanically by scratching ...
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bioRxiv - Biochemistry 2023Quote: ... and then lysed for 10 min at RT in M-PER™ mammalian protein extraction reagent (Cat.# 78501, Thermo Scientific) supplemented with 1X cOmplete ...
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bioRxiv - Cell Biology 2022Quote: ... with a Cadenza CD-C18 3 μm packing column (Imtakt, 2.1 mm id x 150 mm) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) via an Ion Max ion source with a HESI II probe (Thermo Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... and 2 M NaCl) were incubated in a dialysis chamber (Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO, Thermo Scientific) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Linearized plasmids were used as template for in vitro synthesis of mRNA using the SP6 m message machine kit (Ambion) and purified using the Megaclear Kit (Life technology Ambion).
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bioRxiv - Biochemistry 2023Quote: ... 200 pmol of the annealed primed templates were combined with 1 mg of Dynabeads M-280 Streptavidin beads (ThermoFisher Scientific) in 200 μl of Buffer B (40 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with fluorescently labelled secondary antibodies (1:1,000 G-anti-Rb-AlexaFluor-488, G-anti-M-AlexaFluor-555, ThermoFisher Scientific) and DAPI in PBS+ for 1.5 hr at room temperature before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in DPBS buffer (Dulbecco’s PBS 0,01 M + 0,2% BSA) and afterwards for another 30 minutes blocked with 5% Normal Goat Serum (Thermo Fisher Scientific) and 0,25% (0,2% for crypts ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μl of 100 μM reconstituted samples were then injected onto a Superdex200 increase 10/300 GL column using a DIONEX 3000 system (ThermoFisher) consisting of a DIONEX UltiMate 3000 UHPLC pump in line with a DIONEX UltiMate 3000 Diode Array Detector (Figure 8A-B) ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Immunology 2022Quote: ... The resulting RNA was then primed with random hexamers and reverse transcribed using RevertAid M-MuLV reverse transcriptase (Thermo Fisher). 10 μl of each sample was pooled and then serially diluted to generate a set of standards that were later used to create a standard curve in each experiment ...
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bioRxiv - Cancer Biology 2023Quote: B cells were isolated from the spleen by using EasySep™ Mouse B Cell Isolation Kit (19854, STEMCELL) and cultured in RPMI supplemented with 0.1 M non-essential amino acids (11140050, Thermo Fisher), 1LmM sodium pyruvate (11360070 ...
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bioRxiv - Biochemistry 2023Quote: ... Following digestion with BsaI the insert was purified using magnetic streptavidin coupled beads (M-270 Dynabeads, Life Technologies, Carlsbad, CA) and subsequently ligated to phagemid pCD12 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first washed three times in 0.1 M PB and treated with streptavidin conjugated to AlexaFluor488 (5μg/ml) (Molecular Probes #S11223) in 0.1 M PB containing 0.3% Triton X-100 (TX ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with ice-cold 1×DPBS and lysed in M-PER mammalian protein extraction reagent (ThermoFisher, Prod#78505) supplement with protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS and incubated with 50 M of Click-iT homopropargylglycine (HPG) in methionine-free RPMI media (Gibco) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then spun at 1000 x g in a M-20 Microplate Swinging Bucket Rotor (Thermo Fisher Scientific) on a Sorvall Legend XTR centrifuge (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with 2 μl of 2.7 M ammonium bicarbonate before loading on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen) for separation ...
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bioRxiv - Neuroscience 2023Quote: Fly brains were dissected and incubated in 1-μM MitroTracker red dye (kit #M-7512, Thermo Fisher Scientific, Waltham, MA) for 15 mins ...
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bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription mix which contained 0.4 μL of 5X M-MLV reverse transcriptase buffer (250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, Invitrogen), 0.1 μL of 0.1 M DTT (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... All fractions were neutralized with Tris-HCl (0.1 M; pH 9.0) and their protein concentrations were determined by bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Prior to the addition of 74.5 μl of 1 M DTT and 187.5 μl NuPAGE LDS Sample Buffer (4X) (Thermo Fisher Scientific), cells were further mechanically disrupted by passing the lysate through a 26g size needle ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg/µL of anti-Rpb3 or anti-Rpb4 antibody was incubated with 25 µL of magnetic beads (Dynabeads M-280 Sheep anti-Mouse IgG, 11202D, Invitrogen) for 1 h at 4°C in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 times with 6 m/s and 2 times with 6.5 m/s intermitted by cooling on ice using MP Biomedicals™ Lysematrix D (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracted from the cells was dissolved in 0.2 M potassium hydroxide aqueous solution overnight and quantified using Pierce BCA Protein Assay Kit (PI23225, Fisher Scientific). Extracted metabolites are then resuspended in HPLC-grade water containing 5 μM 1,4-piperazinediethanesulfonic acid (PIPES ...
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bioRxiv - Cell Biology 2023Quote: ... and IMCD3-FlpIn-IFT88-NG3 (a gift from M. Nachury, UCSF) cells were grown in DMEM/F-12(HEPES) (Gibco) supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (Thermo Fisher). After 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
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bioRxiv - Neuroscience 2024Quote: ... The ribosomal pellet was resuspended in 600 µl of 10 mM Tris pH 7 (prepared from 1 M Tris pH 7, Ambion) and stored at –80 °C until all samples for each tissue type were processed until this step.
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and a maximum amount of proteins (2.3-4 mg) was loaded on 1 mg of Dynabeads M-280 Streptavidin (ThermoFisher Scientific), previously equilibrated with RIPA buffer ...