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Citations for Thermo Fisher :
5051 - 5100 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...
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bioRxiv - Immunology 2023Quote: ... Sections were then washed with 1% goat serum and 1% triton for 30 minutes and were incubated with the secondary antibody -anti-rat Alexa fluor647 (1:500, ThermoFisher)-for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... sections were probed with biotinylated monoclonal antibody E492 (3 µg/mL; steamer pH 6.1) alongside a monoclonal antibody either against Clec4F (clone 370901, rat IgG2a, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Rat ES cells derived from SD rats’ blastocyst were cultured on feeder cells with N2B27 plus 2il: Mix DMEM/F12 (Gibco) with Neurobasal medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Developmental Biology 2023Quote: ... This was followed by overnight incubation in the goat anti-rat Alexa Fluor 647 secondary antibody (A21247, ThermoFisher, 1:500). The embryos were washed with PBT 4X15 min and kept at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked in 5% BSA in PBS and rat-anti-DILP2 was incubated overnight at 4°C (Geminard et al., 2009) followed by Alexa488 anti-rat-secondary antibodies (Invitrogen). Tissues were washed and mounted in Vectashield with DAPI (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... The hydrogels were washed several times under sterile conditions and incubated with 3.3% of Rat Tail Collagen 1 (Gibco A10483) solution at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... that was manually coated with 50 ug/mL rat tail collagen I (Ref. no. A10483-01, Gibco, Grand Island, NY) prior to cell plating ...
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bioRxiv - Cell Biology 2023Quote: ... followed by additional 15 min incubation with 10 μg/ml Alexa Fluor 647-conjugated goat anti-rat IgG (cat# A-21247, Invitrogen) or goat anti-mouse IgG (cat # A-21235 ...
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bioRxiv - Neuroscience 2023Quote: ... a thin layer of collagen matrix was applied on each coverslip (500µL of 3mg/mL collagen I (from rat tail, Thermo Scientific), 20µL of 1mg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction started upon the addition of 13 μL of microsome mix from the liver of Sprague-Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-488 phalloidin (Invitrogen) if needed ...
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bioRxiv - Immunology 2023Quote: ... and next for 30 min at 37 °C in the presence of chicken anti-rat IgG conjugated with Alexa Fluor 647 (Invitrogen) to stain CD14/IgG on the cell surface and allow its subsequent uptake ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 10% rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated (HRP-linked) secondary antibodies anti-rat IgG (ThermoFisher Scientific, 31470, 1:5000), anti-rabbit IgG (Promega ...
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bioRxiv - Bioengineering 2024Quote: hCMEC/D3 BECs were maintained in tissue culture flasks or multi-well plates pre-coated with 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium comprised of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were diluted in 10% NDS in PBTx and applied overnight: Rabbit anti-Iba1 (1:1,000; Wako; RRID: AB_839504) and Rat anti-GFAP (1:500; Invitrogen; RRID: AB_2532994). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
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bioRxiv - Bioengineering 2024Quote: ... the polymerization of EDOT took place on the rat sciatic nerve constructs using ferric chloride (FeCl3) (Thermo Scientific #Cat: 012357.09) oxidant in the presence of ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... 10 raw files were imported into Proteome Discoverer software for library construction using Swiss-iRT and Uniprot-Rat databases (version 2.0, Thermo Scientific). In the sucrose group ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor555 (anti-mouse) and AlexaFluor488 (anti-rat, both secondary antibodies were from Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) for 45 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used (1:500, goat anti-rat Alexa 488 Invitrogen A11006; 1:500, goat anti-rabbit Alexa 555 Invitrogen A21429 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 3 hours at RT in secondary antibody (anti-rat AlexaFluor-488, #A21208, or anti-mouse AlexaFluor-488, Invitrogen #A11001 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then isolated as CD4 or CD8 respectively with immunomagnetic beads (Dynabeads Mouse CD4 +, Invitrogen, catalog: 11445D; or Dynabeads Sheep anti-Rat IgG, Invitrogen, catalog ...
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bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
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bioRxiv - Neuroscience 2024Quote: ... Remaining cells were washed with PBS and blocked with 10% rat serum and then incubated with following fluorescent monoclonal antibodies from Invitrogen: CD11b eFluor450 (Clone M1/70) ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-mouse IgG (1:300; A21202) or anti-rabbit IgG (1:500; A21206) or anti-rat IgG (1:500; A21208, both Invitrogen), Alexa Fluor 594-labeled donkey anti-goat IgG (1:300 ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate, ThermoFisher Scientific) with a 6 mm diameter dropper ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... After neutralization with 1/10 volume of 1 M sodium phosphate buffer (pH 8) the antibody was coupled to M270 Epoxy Dynabeads (Thermo Scientific) in ammonium sulfate buffer (0.1 M sodium phosphate pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... C11-BODIPY 581/591 was prepared as a 5 μM working solution in HBSS and added to cells along with 1 μg/mL Hoechst 33258 stain (Cat # H3569, Thermo Fisher). Cells were incubated for 10 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide mixtures were analysed by nanoflow reversed-phase liquid chromatography tandem mass spectrometry (LC-MS/MS) on an M-Class HPLC (Waters) coupled to a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher). Peptide mixtures were loaded in buffer A (0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... with 75 μm inner diameter filled with ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) mounted to an Easy-nLC 1200 (Thermo Fisher) and sprayed online into an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H2SO4) and the optical density was measured at a wavelength of 450 (OD450) (MULTISKAN FC, Thermo scientific).
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bioRxiv - Microbiology 2021Quote: ... Partial amplification of AIV-M gene was done using SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen, Germany) and generic real-time reverse-transcription PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription for total RNA was performed using random hexamer primers according to the manufacturer’s instructions using M-MLV reverse transcriptase (Invitrogen, Ref. 28025013). For RT of sRNA’s ...
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bioRxiv - Molecular Biology 2021Quote: ... the selections were conducted using biotinylated α-cobratoxin that was captured using 80 µL of streptavidin-coated Dynabeads (Invitrogen, M-280). The concentration of α-cobratoxin was decreased through the three rounds of selections starting at 10 nM in the first round and ending at 20 pM in the third round ...
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bioRxiv - Immunology 2020Quote: ... Whole-cell lysates were then prepared by washing cells in cold PBS and lysing in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific) with protease/phosphatase inhibitor cocktail (Cell Signaling Technology) ...
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bioRxiv - Developmental Biology 2020Quote: Polyps were incubated with EdU (300 μM from a stock dissolved in DMSO) in artificial seawater for 30 minutes (Click-it Alexa Fluor 488 Kit; Molecular Probes) as previously reported48 ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 500-2000 ng total RNA using RevertAid™ H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers ...
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bioRxiv - Genetics 2022Quote: ... 20 μg of H3K9me2 antibody or 20 μg of H3K9me3 antibody (Hayashi-Takanaka et al. 2011) were preloaded to 40 μl Dynabeads M-280 Sheep anti-Mouse IgG (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... Samples were fixed with 4 % paraformaldehyde in PBS for 20 min at RT followed by a washing step in 0.1 M glycine/PBS (Thermo Fisher Scientific) and permeabilization for 20 min in 0.1 % Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The light and heavy isotope-labeled samples were pooled in 450 µl of IP buffer (Tris-buffered saline [TBS] and 0.1% Tween-20) containing 3 µg of Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) and incubated for 30 min at room temperature (note that the final SDS concentration in the solution was 0.5%) ...