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Citations for Thermo Fisher :
4801 - 4850 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was used to synthesize cDNA using the M-MLV Reverse Transcriptase Kit with Random Primers (Invitrogen). Gene specific primers targeting 18S RNA (forward:AACCCGTTGAACCCCATT ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from 600 ng of total RNA by reverse transcription using M-MLV reverse transcriptase and random primers (Invitrogen). For the qPCR analyses ...
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bioRxiv - Cell Biology 2021Quote: ... a gift from NovoNordisk compound sharing program) that does not cross-react with IGF1R23 was covalently conjugated to Dynabeads M-270 Epoxy beads (Cat. #14301, Invitrogen) to immunoprecipitate INSR according to the manufacture’s protocol ...
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bioRxiv - Genetics 2020Quote: ... peptides were separated on a 20 cm self-packed column with 75-µm inner diameter filled with ReproSil-Pur 120 C18-AQ (Dr.Maisch GmbH) mounted to an EASY HPLC 1000 (Thermo Fisher) and sprayed online into an Q Exactive Plus mass spectrometer (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 5 mM MgCl2 and 0.01 M DTT containing 40 U RNasin Plus and 200 U SuperScript III Reverse Transcriptase (Thermo Fisher). The reaction was incubated at 42 °C for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed 2-3 times in 0.1 M PB then transferred to a solution containing Streptavidin conjugated to AlexaFluor568 (1:500, Invitrogen, UK) vand 0.3% Triton X-100 and 0.05% NaN3 ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was incubated in cell lysates for 45 min at room temperature followed by addition of 50 µL streptavidin Dynabeads M-280 (Invitrogen) for 45 additional minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... washed with cold PBS and lysed with appropriate volume of M-PER™ mammalian protein extraction agent (Thermo Fisher Scientific) supplemented with 1% protease inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Eluates were diluted to 2 M urea in 50 mM ammonium bicarbonate and digested with 1 μg of trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 µl water and precipitated in 0.75 M ammonium-acetate and 10 µg glycogen (#R0551 Thermo Scientific) by adding 125 µl ethanol (100%) ...
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bioRxiv - Cancer Biology 2022Quote: ... Each acyl-CoA was quantified by the [M-507+H]+ product ion with a 5 ppm window with Tracefinder 4.1 (Thermo Scientific). Analysts were blinded to sample identity during sample preparation and analysis.
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 860 μl of methanol (cat. 24299-2,5L - Honeywell) and 690 μl of NaOH (1 M; S/4880/60 – Fisher Scientific) in 6.4 ml of MilliQ water ...
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was isolated from cell pellets as described and 1 μg of total RNA was reverse transcribed to first strand cDNA using the M-MLV Reverse Transcriptase (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and the samples were first postfixed overnight in 4 % PFA (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 d for decalcification ...
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bioRxiv - Microbiology 2022Quote: ... connected to a PepMap C-18 trap-column (0.075 x 50 mm, 3 m particle size, 100 pore size; Thermo Fisher) and an in-house-packed C18 column μ (column material ...
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bioRxiv - Microbiology 2022Quote: ... Samples were analyzed by nano-LC-MS/MS with a Waters M-class HPLC system with a Fusion Lumos (ThermoFisher). Peptides were separated on a trapping column and eluted over an analytical column (75 µm ...
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bioRxiv - Microbiology 2022Quote: ... Prior to the addition of 74.5 μl of 1 M DTT and 187.5 μl NuPAGE LDS Sample Buffer (4X) (Thermo Fisher Scientific), cells were further mechanically disrupted by passing the lysate through a 26g size needle ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Physiology 2022Quote: ... 100 ng to 1 μg of total RNA was reverse-transcribed using the reverse transcriptase M-MLV (28025013, ThermoFisher Scientific) and assessed using the SYBR™ Green PCR Master Mix (4385617 ...
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bioRxiv - Plant Biology 2022Quote: Hydrogen peroxide content was measured in elongating peduncles (50% final length) of Magnif and Magnif M using the protocol described in Amplex Red Hydrogen Peroxide Kit (Invitrogen). Plants were grown in a glasshouse as described in the section “Methods ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was generated from 250 ng of all extracted RNA samples using M-MLV RT (Thermo Fisher Scientific) and treated with RNaseOUT (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated using 50 cm Acclaim PepMap 100 analytical column (75 μm ID, 3 μm C18) in conjunction with a Pepmap trapping column (100μm × 2 cm, 5 μm C18) (Thermo Scientific) analysed with Orbitrap Fusion Tribrid mass spectrometer (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... was generated using an established low-pH model.7 Mid-log Mtb-lux was washed with PBS (1X) and transferred into PCB media: 1X phosphate-citrate buffer (PCB, composed of 0.2 M sodium phosphate (Fisher Scientific) with 0.1 M citric acid (Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were treated with 0.05 M iodoacetamide and digested overnight with 0.5 μg MS Grade Pierce Trypsin Protease (Thermo Fisher Scientific). Digested samples were desalted using stage tips (Rappsilber et al. ...
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with a mixture of 360 μL of 50 mM ammonium bicarbonate (17) and 5 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... PierceTM High Capacity Neutravidin Agarose (Cat. # 29202) and M- PERTM Mammalian Protein Extraction Reagent (Cat. # 78501) were obtained from ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... or TSA (0.5 μM) in the presence or absence of LPS (1 ng/ml) for 16 hours and subsequently lysed in TRIZOL (Invitrogen) and total RNA was extracted using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated from RNA using random hexamers (ThermoFisher Scientific N80801270 and M-MLV reverse transcriptase kit (Invitrogen 28025-013) with the addition of RNaseOUT recombinant ribonuclease inhibitor (ThermoFisher Scientific 10777-019 ...
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bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
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bioRxiv - Microbiology 2024Quote: ... Precipitation of the aqueous layer was accompanied with 1 μl of 0.1 M DTT (18091050) and 15 μg of GlycoBlue reagent (ThermoFisher #AM9516). Purified RNA was subject to a second round of isolation using Qiagen RNA columns (74106 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was centrifuged at 10000 g at 4°C for 10 minutes and supernatant was transferred to Dynabeads M-270 (Invitrogen) coupled to either CTD Ser2P (C15200005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatographic separation was performed on a Vanquish UHPLC system with a Cadenza CD-C18 3 µm packing column (Imtakt, 2.1 mm id x 150 mm) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) via an Ion Max ion source with a HESI II probe (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... with a Cadenza CD-C18 3 µm packing column (Imtakt, 2.1 mm id x 150 mm) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) via an Ion Max ion source with a HESI II probe (Thermo Scientific) ...
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bioRxiv - Genetics 2024Quote: ... tryptic peptides were then separated by reversed-phase XSelect CSH C18 2.5-μm resin (Waters) on an in-line 150 x 0.075–mm column using an UltiMate 3000 RSLCnano system (Thermo Fisher). Peptides were eluted using a 60-minute gradient of buffer A/buffer B from 98:2 to 65:35 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed twice with phosphate buffered saline (PBS) and lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) supplemented with 1% protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... the sample was transferred into a tube previously passivated with denatured BSA blocking buffer and mixed with magnetic beads (Dynabeads M-270 Streptavidin, Invitrogen) coated with DNA strands complementary to a section of the purification linker handles on the triangles ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final) (Thermo Scientific, Rockford, IL, USA) to the M-PER buffer ...
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bioRxiv - Cell Biology 2024Quote: ... cell monolayers were rinsed with ice-cold KPBS buffer (136 mM KCl, 10m M KH2PO4, pH 7.25, supplemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This step was followed by incubation with 50μl of Dynabeads® M-280 sheep anti-mouse IgG (11201D, Thermo Scientific) for 3 hours ...
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bioRxiv - Genomics 2023Quote: ... Cell lysates was denatured under reducing conditions by boiling 30 μg of total protein with 1 M DTT and 4X LDS sample buffer (Invitrogen) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated DRGs were filtered using a 100 μm cell strainer (Fisher) and cultured on collagen-coated glass coverslips in DRG media consisting of Neurobasal A medium (Gibco), 5% fetal bovine serum (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue grinder was rinsed with 1 ml HEB buffer (Hibernate A, ThermoFisher; 10 µl/ml GlutaMAX, ThermoFisher; 0.32 M Sucrose) and the wash filtered into the same tube ...
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bioRxiv - Plant Biology 2023Quote: ... and the eluted chromatin was reverse-crosslinked by adding 20 ul 5 M NaCl and incubated at 65 °C overnight followed by treatment of Proteinase K (Invitrogen) for 4 hours at 45 °C ...
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bioRxiv - Microbiology 2023Quote: ... Cryosectioned lung tissue sections (5 μm/section) from infected mice were stained using polyclonal anti-Ax rabbit sera and appropriate fluorescent conjugate secondary antibody (Invitrogen). Tight junctions were stained via a polyclonal goat antibody against ZO-1 and relevant secondary antibody (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... was loaded with a magnetic microbead solution (1.5 μl, diameter: 4.5 μm, 2.4 × 106 beads, Dynabeads M-450 Epoxy, ThermoFisher Scientific, Waltham, MA). Using a micromanipulator ...