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Citations for Thermo Fisher :
5151 - 5200 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Glycine (0.1 M) was added to quench the reaction and the buffer was exchanged for PBS using a Zeba spin column (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... DNA was precipitated from each fraction with PEG-NaCl (30% PEG, 1.6 M NaCl) and linear polyacrylamide (Invitrogen, Thermo Fisher Scientific), washed with 70% ethanol ...
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bioRxiv - Microbiology 2021Quote: ... DNA was precipitated from each fraction with PEG-NaCl (30% PEG, 1.6 M NaCl) and linear polyacrylamide (Invitrogen, Thermo Fisher Scientific), washed with 70% ethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-PrimPol antibody (Table 1) was coupled to magnetic M-270 Epoxy Dynabeads® (7.5 μg antibody/ mg beads; Life Technologies), and IPs were performed following manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.15 M NaCl) containing 0.05% Tween 20 and counterstained with anti-rabbit IgG Alexa Fluor 488 (Life Technologies, Cat # A-11034). The stained fungi were imaged with Leica confocal microscope at excitation wavelength of 488 nm ...
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bioRxiv - Microbiology 2020Quote: ... Glycine (0.1 M) was added to quench the reaction and the buffer was exchanged for PBS using a Zeba spin column (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... complemented with 50 µM Alexa Fluor 594 and 100 µM Oregon Green BAPTA-1 (OGB-1) or Oregon Green BAPTA-6F (OGB-6F) (all fluorescent dyes from Invitrogen-Molecular Probes). Electrophysiological results were similar using OGB-1 and OGB-6F and therefore results obtained with different Ca2+-sensitive dyes were pooled.
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bioRxiv - Immunology 2020Quote: ... Punch biopsy skin samples were obtained from XenoSCID and protein extracted and homogenised using M-PER mammalian protein extraction reagent (Thermo Scientific) and two 7mm metal beads ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed in 0.1 M TB (2 ×5 minutes) and counterstained with Hoechst 33342 (1:20000, #62249, Thermo Fisher Scientific) diluted in 0.1 M TB ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed three times in 0.1 M PBS and incubated for 2 h at room temperature in donkey anti-goat AlexaFluor546 (1:500, Invitrogen, #A-11056), donkey anti-goat FITC (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µL of vortexed 10 mg/mL 2.8 µm paramagnetic beads (Dynabeads M-280 Streptavidin, Thermo Fisher Scientific Baltics UAB, Norway) was added to 0.1-1.0 pg/µL solution of nucleosome arrays in 500 µL of MB followed by incubation for 10 min with gentle mixing ...
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bioRxiv - Molecular Biology 2022Quote: ... peptides were separated on a C18 reversed phase 15 cm length × 7 μm internal diameter C18RP analytical column (Acclaim PepMap RSLC, C18 ReproSil AQ, 1.9 μm particles, 120 Å pore size, Thermo Scientific) at 45°C using a linear gradient of 3–45% ACN 0.1% acetic acid in 60 minutes at a flow rate of 500 nl/min ...
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bioRxiv - Neuroscience 2022Quote: ... The following day sections were again washed (3 × 5 min washes with 0.1 M PBS) and incubated with secondary fluorophore-conjugated antibody Alexa 555 goat anti-mouse (1:500, Thermo Fisher Scientific) for visualization of each primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was then synthesized using 1 mg of total RNA and M-MLV Reverse Transcriptase according to manufacturer’s instructions (Invitrogen, Waltham, MA).
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bioRxiv - Developmental Biology 2022Quote: 500ng of peptides per sample was analyzed by using an M-class nanoAquity UPLC system (Waters) connected to a Q Exactive mass spectrometer (Thermo Scientific). Buffer A consisted of Water/0.1% FA and Buffer B consisted of ACN/0.1% FA ...
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bioRxiv - Biophysics 2019Quote: ... Single strands were separated by electrophoresis in a 1.2% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific). After electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The CO2 produced was measured by titrating KOH solutions with 0.5 M HCl using a potentiometric titrator (Orion Star T900, Thermo Scientific, USA). The conversion of PU to CO2 was estimated by subtracting the average amount of CO2 produced in the blank to the amount of CO2 produced in the PU-containing jars.
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bioRxiv - Biochemistry 2020Quote: ... remaining samples were diluted to 5 μM and UV/Vis spectra were recorded between 340 and 800 nm using an EvolutionBio 260 UV/Vis spectrophotometer (ThermoFisher Scientific). The spectra shown in Fig ...
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bioRxiv - Neuroscience 2019Quote: ... dual injections of Alexa Fluor 488- and 555-conjugated CTBs (0.5% in 0.1 M phosphate buffer, Thermo Fisher Scientific, Waltham, MA) were made iontophoretically by 70 pulses ...
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bioRxiv - Cell Biology 2020Quote: ... For data analysis peak areas of extracted ion chromatograms of each isotope of compound-specific fragments [M - e-]+ were determined using the TraceFinder software (Version 4.2, Thermo Fisher Scientific) with a mass accuracy (<5 ppm) ...
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bioRxiv - Microbiology 2020Quote: ... Glycine (0.1 M) was added to quench the reaction and the buffer was exchanged for PBS using a Zeba spin column (Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... BMDCs were incubated for 15 min with LPS-coated latex beads or 3 µm magnetic beads (Dynabeads M-280 streptavidin, Invitrogen, NY) and then chased ...
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bioRxiv - Genomics 2020Quote: ... 4 μM glass slide) then for Laser Capture Microdissection (LCM; N=3; 7 μM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522). The slides were stored at −20°C in an airtight container with desiccant until ready for dissection (1 day to 3 months) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen], 0.5 μl 0.1 M DTT, 0.25 μl Superscript IV reverse transcriptase [Invitrogen], 0.25 μl of RNasin) and incubation for 5 min at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... were analysed by nanoflow reversed-phase liquid chromatography tandem mass spectrometry (LC-MS/MS) on an M-Class HPLC (Waters) coupled to a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher). Peptide mixtures were loaded in buffer A (0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of lysate were incubated with 60 µl of lysis buffer-equilibrated Dynabeads M-280 Streptavidin (Thermo Fisher Scientific, 11206D) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Biophysics 2019Quote: ... cell lines were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, M&C GENE TECHNOLOGY LTD, BEIJING, China) containing with 10% heat-inactivated fetal bovine serum (FBS, GIBCO, USA) and 100 U/ml penicillin and 100 μg/ml streptomycin solution(PS ...
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bioRxiv - Microbiology 2019Quote: ... Then, 15 mL of a 0.2 M KOH (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) and methanol (Fisher Scientific Worldwide (Shanghai) Co. ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Physiology 2020Quote: ... Sections were then washed and incubated in streptavidin-conjugated Alexa Fluor® 488 (diluted 1:2000 in 0.1 M PB, A21370, Life Technologies) for 2 hr at RT ...
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bioRxiv - Physiology 2020Quote: ... sections were washed and incubated with anti-rabbit Alexa Fluor® 594 (diluted 1:500 in 0.1 M PB, A11037, Life Technologies) for 2 hr at RT ...
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bioRxiv - Physiology 2020Quote: ... Sections were then washed and incubated in streptavidin-conjugated Alexa Fluor® 488 (diluted 1:2000 in 0.1 M PB, A21370, Life Technologies) for 2 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... All metabolites were detected across a mass range of 70-1050 m/z using a Q Exactive Orbitrap instrument (ThermoFisher Scientific) with heated electrospray ionization and polarity switching mode at a resolution of 70,000 (at 200 m/z) ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... PSCs were incubated 1 hour with bESCM containing 10 μM Y-27632 prior to dissociating then washed twice with PBS and incubated for 3 minutes in TrypLE Express (Gibco, #12604013) at 37°C/5%CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... the theoretical m/z values for all peptides were used to interrogate the raw data using the Quan Browser software program (Thermo Scientific) (Table 1) ...
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bioRxiv - Neuroscience 2021Quote: ... 14 μm sections were cut on a cryostat and briefly washed with 1X PBS before mounting on Superfrost Plus slides (Fisher Scientific). The slides were dried at −20°C and stored at −80°C before use ...
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bioRxiv - Neuroscience 2021Quote: ... frozen on dry ice and 30 μm sections of mouse striatum and ventral midbrains encompassing the SNpc were prepared using an HM 560 Microm cryostat (Thermo Scientific).
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bioRxiv - Biophysics 2020Quote: ... P{w[+m*]=Sgs3-GFP}2 larvae were let to pupate as described for measurement assays but on circle cover slips (Thermo Scientific, 32 mm diameter ...
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bioRxiv - Immunology 2020Quote: Eluting peptides were converted to gaseous ions via a fused silica Picotip 5 cm x 10 μm ID emitter (NewObjective) for ESI (spray voltage, 2 kV; heated capillary, 300 °C) using a nano-electrospray source (Thermo Scientific). MS spectra were acquired in the positive ion mode on the Orbitrap Fusion MS with an m/z range of 375–1,700 with R of 120,000 at m/z 200 ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody ...
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bioRxiv - Genetics 2020Quote: ... were incubated at 60°C overnight in the presence of 10 mM Tris pH 7.5 and 100 mM KCl and then conjugated to Streptavidin-Coupled M-280 Dynabeads® (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cross-linking occured for 30 min in a rolling tube after which beads were washed with 0.2 M ethanol amine pH 8 (#149582500, Thermo Fisher Scientific) to quench the crosslinking reaction.
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bioRxiv - Biochemistry 2022Quote: ... Urea concentration was then reduced to 1.5 M by dilution with 50 mM NH4HCO3 and samples were digested with trypsin (Thermo Scientific Pierce) (Shevchenko et al. ...
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bioRxiv - Bioengineering 2022Quote: ... Serial dilutions of gene fragments were performed in sheared salmon sperm DNA at 5 µg mL−1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Cancer Biology 2022Quote: Aliquots (125 μL) of sEV- or m/lEV-fractions in PBS were stained with 5 μM CellTrace Violet (CTV; C34571, Thermo Fisher) for 10 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... 2-5 μg of antibody was bound to pre-blocked (with 0.5% BSA) magnetic Dynabeads M-280 sheep anti-Mouse IgG or sheep anti-Rabbit IgG (Thermo Fisher Scientific) and applied to the diluted ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...