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Citations for Thermo Fisher :
4751 - 4800 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... DNase-treated RNA samples (1 μg) were converted to complementary DNA (cDNA) using the M-MLV enzyme (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... cells were cross-linked for 15 minutes at room temperature with 1% formaldehyde and quenched for 5 mins at room temperature with 0.2 M glycine (Thermo Fisher). The cross-linked cells were aliquot (~ 3X106 cells ...
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bioRxiv - Cell Biology 2022Quote: In CD protocol 2 hESCs at 80%-90% confluency in 6-well plates were lifted with 1 ml/well UltraPure 0.5 M EDTA (Invitrogen, 15575038), counted ...
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bioRxiv - Neuroscience 2022Quote: Dry protein pellets of both ovary and hemolymph samples were dissolved and reduced in 8 M urea (Fisher Scientific, 45000234)/70 mM ammonium bicarbonate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GE Health Sciences) surface was conditioned by injecting 350 mM EDTA (diluted from 0.5 M, pH 8 stock, no. 15575020; Thermo Fisher) at 10 ul/min for 1 minute followed by extensive washing with running buffer to remove residual EDTA ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 2 ug of RNA was used as a template for cDNA synthesis using M-MLV reverse transcriptase (#28025, Invitrogen) and oligo-dT as primer ...
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bioRxiv - Cell Biology 2022Quote: ... was cultured in a gelatin/fibronectin matrix (5 μg fibronectin / 0.02% gelatin (m/v)-Sigma) and Claycomb culture medium supplemented with Glutamax™ (Gibco), 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: MS analyses were performed on an ACQUITY UPLC M-Class System (waters) coupled to an Orbitrap Fusion Lumos (Thermo Fisher). For LC-MS/MS injections of proteome and phosphoproteome samples ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 and SUM149PT whole cell lysates were isolated from spheroids grown for seven days using mammalian protein extraction reagent (M-PER; ThermoFisher) following manufacturer protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... the resulting pellets were washed twice with 0.1 M ammonium acetate in 80% methanol and dried under vacuum (Genevac centrifugation concentrator miVac Duo, Fisher Scientific). The pellets were resuspended in 100 µl of 50 mM ammonium bicarbonate and submitted to reduction (5 mM dithiothreitol ...
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bioRxiv - Molecular Biology 2019Quote: ... U2OS cells stably expressing GFP tagged SESN2 were exposed to 10 μM FCCP treatment for 24h and before the fluorescent microscopy procedure were incubated with 100 nM MitoTracker Red (CMXRos, Invitrogen) in DMEM for 30 min at 37°C to stain mitochondria.
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bioRxiv - Plant Biology 2021Quote: ... five-day-old thalli expressing each of the markers were stained with 0.5 M MitoTrackerTM Orange CMTMRos (Thermo Fisher Scientific) for 20 minutes and washed three times with 1/2× Gamborg’s B5 liquid medium ...
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bioRxiv - Neuroscience 2021Quote: ... 500 ng of RNA was reversely transcribed using random primers (Eurofins) and Moloney murine leukaemia virus reverse transcriptase (M-MLV, Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Synthesis of complementary DNA (cDNA) with M-MLV Reverse Transcriptase (SuperScript III First Strand Synthesis Supermix, 18080-400, Thermo Scientific) was primed with random hexamers ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) antibody diluted in the blocking solution for 2H at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) diluted in blocking solution for 2H ...
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bioRxiv - Bioengineering 2020Quote: ... The solution pH was adjusted to 7.4 by dropwise additions of 0.1 M HCl or NaOH solution and measured with a pH meter (Model 13-641-253, Thermo Scientific Orion 720A+ ...
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bioRxiv - Cell Biology 2022Quote: ... then the biotin-depleted “flow-though2” was incubated with 100 μL Dynabeads™ M-280 Streptavidin (Thermo Fisher Scientific, 11205D) for 2 h at 4°C with gentle rotation ...
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APOE ε4 gene dose effect on imaging and blood biomarkers of glial reactivity and β-amyloid pathologybioRxiv - Neuroscience 2022Quote: ... coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads and a KingFisher Flex instrument (Thermo Fisher Scientific), and further analyzed by liquid chromatography-tandem mass spectrometry (LC–MS/MS) ...
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bioRxiv - Cancer Biology 2022Quote: ... or normal IgG (1μg) was incubated with 50 μl Dynabeads M-280 sheep antimouse IgG magnetic beads (Thermo Fisher, 11202D) in 200ul wash buffer (50 mM pH 7.5 Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: Data acquisitions begin with a survey scan (m/z 380 −1800) acquired on a Q-Exactive Orbitrap mass spectrometer (Thermofisher) at a resolution of 120,000 in the off-axis Orbitrap segment (MS1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... of DNA was prepared with specific combinations of plasmids and brought up to a volume of 100 µL using 0.15 M sodium chloride (NaCl, Fisher Scientific). Next ...
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bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... 10 μm thick frozen sections were cut on a Microm HM 505 E cryostat and mounted on Superfrost Plus slides (ThermoFisher). Sections were stained with the following primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: Tumor tissue and organ tissues was flash frozen in liquid nitrogen and homogenized in M-PER lysis buffer (Thermo Scientific) plus Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... We washed the solvent samples five times with an equal amount of 0.3 M sodium borate (Acros Organics, Geel, Belgium) (pH = 9 ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared by centrifugation at 15,000 g for 30 min at 4 °C and the resulting supernatant incubated for 17 h at 4 °C on an end-to end rotator with 50 μl magnetic M-280 streptavidin Dynabeads (Dynal, Invitrogen). Beads were isolated from the supernatant and a sample of the supernatant was mixed with 10x sample buffer (0.5 M Tris-HCl pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: All live imaging of NIH-3T3 cells (Figs 4a-d, 5a-j,l-m, S10, S11) was performed in Leibovitz’s L-15 medium (Gibco, 11415064) supplemented with 10% Donor Bovine Serum and HEPES (Gibco ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μm-width sections were stained with 10μg.mL−1 wheat germ agglutinin (WGA)–Alexa Fluor® 488 Conjugate (W21404, ThermoFisher, France) and 1μg.mL−1 propidium iodide (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... 200 µM of the scaffold DNA and 3x molar excess of BamHI_comp strand were hybridized in FastDigest Green buffer (Thermo Scientific) by heating the sample to 90 °C followed by gradual cooling to 20 °C over 2 h ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of the polar and non-polar phases were injected onto a 30 m column (TG-5MS, Thermo Scientific) using a 10:1 split ratio ...
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bioRxiv - Bioengineering 2019Quote: ... HCT116 and MDA-MB-231 whole cell lysates were isolated from flasks using mammalian protein extraction reagent (M-PER; ThermoFisher) following manufacturer protocols ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... 5 μl of each fraction (polysomes and non polysomes) was used for First-strand synthesis using p(dN)6 and 100 U of M-MLV RT (Invitrogen). The same amount of first strand DNA was used for qRT-PCR analysis ...
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bioRxiv - Genetics 2019Quote: ... in 0.1 M sodium bicarbonate that was incubated with 98 μg of Alexa Fluor 647 succinimidyl ester (A20006, Thermofisher Scientific) for 1h at room temperature (3:1 dye:protein mole ratio) ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from cell pellets and 1 μg of total RNA was reverse transcribed to first strand cDNA using the M-MLV Reverse Transcriptase (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Cell Biology 2020Quote: ... the buffer was exchanged for the 0.1 M ammonium acetate using a Zeba column 40K (Thermo Fisher Scientific, Waltham, Massachusetts), wash steps completed per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Molecular Biology 2021Quote: ... The urea was then diluted to 1.5 M and samples further digested at 37°C overnight with 1:25 w:w trypsin (Thermo Fisher Scientific). Proteinase activity was stopped with formic acid ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was pre-cleared by incubation with 60 µL streptavidin-coupled magnetic beads (Dynabeads M-280, Thermo Fisher Scientific) 1 h at 4 °C on a rotating wheel ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Microbiology 2021Quote: ... 0.01% Tween 20 and 50μM acetyl phosphate) containing 10 μl of pre-washed (3X) magnetic cobalt beads (Dynabeads His-Tag Isolation and Pulldown - Invitrogen) for a final volume of 200 μl in 96-well plates and incubated for 40 min at room temperature on a rotating wheel ...
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bioRxiv - Immunology 2020Quote: ... 100 ng to 1 µg total RNA was subjected to reverse transcription using random primers and M-MLV RT (Invitrogen). Specific primer pairs for each gene were designed ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 M Tris–Cl pH 7.6) for 45 min before being stained with SYBR Gold (cat # S-11494, Life Technologies) for 40 min and de-stained for 30 min before imaging55.
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bioRxiv - Molecular Biology 2020Quote: One μg of DNase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific) according to manufacturer’s protocol with the use of random hexamers to prime the reaction ...
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bioRxiv - Immunology 2021Quote: Immunoprecipitations for competition assays were performed with 8 μg of C-terminally biotinylated nanobody on M-280 streptavidin magnetic beads (Dynabeads, Invitrogen) and 10 μg of spike or RBD preincubated with 10 μg of the indicated (HIS-tagged ...