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Citations for Thermo Fisher :
4701 - 4750 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and pieces incubated in secondary antibodies [anti-rat secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rabbit 568 secondary antibody (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Ki67 (1:500, rabbit polyclonal, ab15580 Abcam or 1:500, rat monoclonal, 14-5698-82 Thermo Fisher Scientific); anti-RFP (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Stretched rat bladders were pinned out on rubber mats and treated with 2.5mg/ml of Dispase (Gibco-ThermoFisher Scientific) dissolved in Minimal Essential Medium (Gibco-ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Stretched rat bladders were pinned out on rubber mats and treated with 2.5mg/ml of Dispase (Gibco-ThermoFisher Scientific) dissolved in Minimal Essential Medium (Gibco-ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS before adding Donkey anti-Rat Alexa Fluor 488 secondary antibody (1:1000; Invitrogen; A21208) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... then with anti-rabbit IgG-Alexa Fluor 488 or anti-rat IgG-Alexa Fluor 594 (1:1000, Invitrogen, USA) for 2 h at room temperature (RT) ...
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bioRxiv - Systems Biology 2024Quote: Cortices of adult rats (Sprague–Dawley) were dissected and a synaptosome preparation was generated using Syn-PER (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Mouse 568, Donkey anti-Rabbit 657, Donkey anti-Rat 488, Donkey anti-Goat 568; all at 1:1,000; Invitrogen) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate ...
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bioRxiv - Microbiology 2020Quote: ... at which point the aqueous layer was removed and transferred to a new tube containing 100 μl of 5 M ammonium acetate (Ambion), 20 μl of 5 mg/ml glycogen (Ambion) ...
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bioRxiv - Cancer Biology 2021Quote: Tumor tissue and organ tissues was flash frozen in liquid nitrogen and homogenized in M-PER lysis buffer (Thermo Scientific) plus Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... Tipless MLCT-D probes (0.03 N/m spring constant) were functionalized with 10 μm diameter borosilicate glass beads (Thermo Scientific 9010 ...
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bioRxiv - Developmental Biology 2021Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg total RNA was used to synthesize the first-strand cDNA with M-MLV reverse transcription kit (Invitrogen, USA). RT-qPCR was performed on a LightCycler480 machine (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized from 3μg of RNA using a M-MLV Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA) and oligo dT ...
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bioRxiv - Developmental Biology 2020Quote: ... and precipitated with 150 μL isopropanol overnight -80°C after addition of 10 μL 3 M NaOAc pH 5.5 and 1.5 μL 15 mg/mL GlycoBlue Coprecipitant (Thermo Fisher, AM9515). The samples were then centrifuged at 21,000g ...
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bioRxiv - Developmental Biology 2020Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Immunology 2022Quote: Freshly isolated platelets were fixed with 4% paraformaldehyde (PFA) in PBS (Morphisto GmbH, Frankfurt a. M., Germany) for 10 min and subsequently permeabilized using 1x Perm buffer (Invitrogen) for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Mononucleosomes were assembled from a linear salt gradient dialysis (2 M to 50 mM KCl) using Slide-A-Lyser Mini Dialysis units with a 7,000 molecular weight cutoff (Thermo Scientific) essentially as described (Clapier et al ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were alkylated with 0.05 M IAA and digested with 0.5 μg MS Grade Pierce Trypsin Protease (Thermo Fisher Scientific) overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 ul of sample was taken out and 20 μl of 5 M NaCl and 10 μl of 10 mg/ml proteinase K (Invitrogen) was then added followed by an overnight incubation on 65°C ...
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bioRxiv - Immunology 2022Quote: ... 3.5 μm pore size) mounted on an M-Class Ultra Performance Liquid Chromatography (UPLC) system (Waters)(total) or Vanquish Horizon UPLC system (ThermoFisher)(phospho) ...
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bioRxiv - Cancer Biology 2020Quote: ... coupled to an analytical column (EASY-Spray PepMap column, 50 × m × 75 μm ID, 2 μm, 100 Å, Thermo Scientific). Peptides were gradient eluted into a Q Exactive (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Larvae were placed in a 6 cm Petri-dish containing a mixture of 0.2 M sucrose and 0.08% Brilliant Blue R dye (Acros Organics, #191490050). The larvae were allowed to feed on the sugar solution for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... 1988) coupled to 10ul magnetic beads (Dynabeads™ M-280 Sheep Anti-Mouse IgG) and resuspened in 500ul TRIzol (Invitrogen) and stored at -80°C.
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bioRxiv - Neuroscience 2020Quote: ... or Clstn3fl/fl::Nestin-Cre (Nestin-Clstn3) mice were homogenized in 0.32 M sucrose/1 mM MgCl2 containing a protease inhibitor cocktail (Thermo-Fisher Scientific) using a Precellys Evolution tissue homogenizer (Bertin Co.) ...
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bioRxiv - Genomics 2019Quote: Total RNA was isolated using Trizol and cDNA was reverse transcribed using M-MLV Reverse Transcriptase (Thermo Fisher Scientific 28025) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and the biotinylated nascent transcripts were isolated in a total of three rounds of streptavidin-coated magnetic bead (M-280, Invitrogen) purifications ...
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bioRxiv - Genetics 2020Quote: Protein lysates were obtained from either pestle-homogenized tissues or cell lysates in M-PER lysis buffer (ThermoFisher; Cat# 78501) supplemented with 1x Complete Mini EDTA-Free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... were lysed in urea buffer (8 M urea, 100 mM NaHPO4, pH 8.5) with HALT protease and phosphatase inhibitor (Pierce/Thermo Scientific) and processed at the Emory Proteomics Core ...
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Microbiology 2021Quote: ... The cell extracts were afterwards incubated with 40 μl of IgG-coupled magnetic beads (Dynabeads™ M-280 Tosylactivated, Invitrogen) for 3 hours at 4°C and the flow-through (unbound ...
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bioRxiv - Systems Biology 2021Quote: ... solid and liquid media were supplemented with 25 or 50 μg m/−1 kanamycin sulfate (Thermo Fisher Scientific, Waltham, MA) for Mycobacterium and E ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed with 5x RT-buffer mix with M-MuLV- RH reverse transcriptase (Biolabmix) and random hexamer primer (Invitrogen) and diluted 1:10 in MilliQ H2O ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mg of fusion protein was dialyzed in 0.1 M NaCO3 pH 9 then mixed with 25 μg of FITC (ACROS organics) in a total volume of 0.5 mL ...
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Fractions containing purified protein were pooled and dialyzed overnight against storage buffer (0.02 M sodium phosphate, pH 7) in a 10 kD cut off dialysis cassette (ThermoFisher, 66380). Purified protein was stored at 4 °C for use within days or at −20 °C for longer-term storage.
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bioRxiv - Neuroscience 2020Quote: ... cDNA were transcribed from 1 μg DNA-free total RNA using the M-MLV reversible transcriptase (Invitrogen, Cat. #28025-013) and oligo(dT ...
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bioRxiv - Neuroscience 2021Quote: ... Cleared lysate (twice centrifugation for 10 min @ 20000 g) was incubated for three hours with 50 µl Dynabeads M-280 Streptavidin (Invitrogen) followed by three washing steps with lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Complementary DNA (cDNA) synthesis and qRT-PCR were performed either as previously described [24] or using M-MLV (Life Technologies) followed by q-PCR performed with SYBR Green PCR Master Mix (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Mcm3 antibody (M038-3; MBL) and anti-mouse IgG conjugated to Dynabeads M-280 (Thermo Fisher Scientific) were used as the primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Plant Biology 2021Quote: ... After running the organic phase in a column (lenght: 60 m, inner diameter: 0.25 mm, 0.25 µm liquid membrane thickness, TR-FAME, Thermo Scientific, France) at 150 °C for 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... The concentration of the free ATP in the dilute phase was evaluated by measuring its absorbance at 259 nm using the molar extinction coefficient of ATP (15,400 M-1 cm-1) using UV-Vis absorption spectroscopy (NanoDrop 2000/2000c Spectrophotometer, Thermo Scientific). The concentration of ATP inside the coacervates was calculated as cdense = (c − cdilutef)/(1 − f) ...
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bioRxiv - Cell Biology 2021Quote: ... the buffer was exchanged for the 0.1 M ammonium acetate using a Zeba column 40K (Thermo Fisher Scientific, Waltham, Massachusetts), wash steps completed per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was reverse transcribed into cDNA using random hexameric primers and the M-MLV reverse transcriptase (Thermo Fisher Scientific) according to the following program ...
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bioRxiv - Microbiology 2021Quote: ... MS spectra of protein ions of different charge-states were acquired in positive ion mode with a resolution setting of 120,000 (at m/z 200) and accurate mass was deconvoluted using Xcalibur (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h in 0.1 M PBS-Tx 0.2% containing 10% normal goat serum (1:100 ThermoFisher, Melbourne, Australia) to block non-specific binding ...
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bioRxiv - Physiology 2021Quote: ... Vero E6 cells (ATCC#CRL-1586; a kind gift of Prof. M. Bouloy, Institut Pasteur, France) were maintained in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... First-strand complementary DNA (cDNA) synthesis was performed with the RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific) using random primers ...