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Citations for Thermo Fisher :
4501 - 4550 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was removed and pellets were then resuspended in 1 mL IP buffer (50 mM Tris Base, 150 mM NaCl, 0.5 M EDTA, pH 7.4) with PierceTM protease (Thermo Scientific) and PierceTM phosphatase (Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries in the flowthrough were precipitated at −20 °C overnight in ethanol with 0.3 M sodium acetate and 1 µl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on a Tapestation (Agilent Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... The biotinylated primers and undigested library members were removed using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, Waltham, MA), using the recommended “Immobilize nucleic acids” protocol ...
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bioRxiv - Genetics 2021Quote: ... Newly synthesized transcripts were selected for biotin-16-UTP incorporation using streptavidin coated magnetic beads (ThermoFisher, Dynabeads M-280). Beads were extracted using QIAzol reagent ...
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bioRxiv - Pathology 2020Quote: ... by mixing 100 μL of lysate with 100 μL of D-luciferin (0,1 mg/mL) supplemented with ATP (0,5 M) in flat bottom 96-well black plates (Thermo Scientific). Luciferase activity was measured for 1 min with f/stop = 1 and binning = 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred into 15% and 30% glycerol and embedded between two coverslips using 120m m Secure-Seal spacers (Invitrogen, S24737) and ProLong Gold antifade reagent (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... equipped with a flame-ionization detector (FID) and a TG-5MS column (0.25 mm id x 30 m x 0.25 µm film thickness; Thermo Fisher). The temperature program was as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysate was incubated with 60 µl of lysis buffer-equilibrated Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, 65305) for 30 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... and the pellet was resuspended in HBS buffer and incubated with Dynabeads® M-280 Streptavidin beads (Invitrogen, # 11205D) for 30 minutes with end-to-end rotation at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were incubated at 30°C for 45 mins and then stopped by adding 120µl of STOP buffer (0.3 M NaAC, 5 mM EDTA, 0.1% SDS, 40 µg/ml linear acrylamide (Ambion 9520)) and 1 µl of Proteinase K (20mg/ml) ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was purified from pooled serum with UF6b-conjugated Dynabeads™ M-270 Epoxy (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... the hybridized DNA fragments were captured with streptavidin-coupled magnetic beads (Dynabeads M-280 streptavidin, Thermo Fisher Scientific, USA), washed ...
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Developmental Biology 2021Quote: ... First-strand cDNAs were synthesized with 1000 or 280 ng of total RNA using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and for nucleic acids using a 12.5 % polyacrylamide/8 M urea gel visualized by SYBR Gold staining (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: Splenocytes and thymocytes were harvested by mechanical disruption on 70 μm cell strainers followed by red blood cell (RBC) lysis and washing with Hank’s Balanced Salt Solution (HBSS) (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using M-MLV reverse transcriptase (Thermo Fisher Scientific, Waltham, MA). Real-time PCR was performed using the QuantiNova SYBR Green RT-PCR Kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... The nanopipettes were all filled with 0.3nM dsDNA diluted in either 0.1 M KCl (P/4240/60; Fisher Scientific), 0.1 M LiCl (CHE2360 ...
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bioRxiv - Biochemistry 2021Quote: ... and 657 m/z were manually inspected using the GlycoPAT and Xcalibur Qual browser (version 4.2, Thermo Fisher Scientific). List of O-GlcNAcylated and un-O-GlcNAcylated peptides and their annotated MS/MS spectra generated by the GlycoPAT software are provided in supplemental Tables S2 and S3 ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting pellet was lysed with 10 mL/g of ice-cold M-PER reagent (Thermo Fisher Scientific # 78505) containing 1X protease inhibitor (Thermo Fisher Scientific # A32953) ...
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bioRxiv - Developmental Biology 2021Quote: Cell extracts and tissue extracts were prepared using M-PER mammalian protein extraction buffer (Thermo Fisher 78501, Waltham, MA) or T-PER tissue protein extraction buffer (Thermo Fisher 78510) ...
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bioRxiv - Biophysics 2021Quote: HeLa cells stably expressing m-Cherry-H2B (reported previously (31)) were maintained in DMEM (Dulbecco’s Modified Eagle’s medium) with GlutaMAX (Life Technologies) supplemented with 10% (vol/vol ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed with standard procedures on 4 μg total RNA with 200 u M-MLV RT (Invitrogen), using the primers listed in Table SX ...
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bioRxiv - Immunology 2020Quote: 1×105 A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA(D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior a second round of reverse transfection ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... hippocampal sections were immersed in 0.05 M glycine (Fisher BioReagents cat# BP3815) in 0.3% TritonX (Thermo Fisher, cat# PI85112) in 1X PBS (T-PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were exchanged into 0.1 M PBS-bicarbonate buffer (pH 8.4) using a 40 k Zeba column (87765 Thermo Fisher) and incubated with a 5- to 12-fold molar excess of the SAFE probe with 10% DMSO for 25 minutes at room temperature in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... Air-dried silk fibroin (SF) was dissolved in 9 M LiBr (199870025, Acros, Thermo Fisher Scientific, Breda, The Netherlands), filtered ...
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bioRxiv - Biophysics 2022Quote: The nanopipettes were all filled with 0.1 ng/μl dsDNA diluted in 0.1 M KCl (P/4240/60; Fisher Scientific) and fitted with a Ag/AgCl working electrode ...
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bioRxiv - Genomics 2022Quote: ... equipped with a flame-ionization detector (FID) and a TG-5MS column (0.25 mm id x 30 m × 0.25 µm film thickness; Thermo Fisher). The oven temperature was programmed at 60°C for 1 min ...
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bioRxiv - Genomics 2022Quote: ... Mass spectra between 350 to 1300 m/z were collected on a Q-Exactive HFX mass spectrometer (Thermo Scientific) in Top20 mode with a full-MS and dd-MS2 resolution of 120,000 and 15,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were lysed in 8 M urea lysis buffer (8 M urea, 10mM Tris, 100 mM NaH2PO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher). Homogenization was performed using a Bullet Blender (Next Advance ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were extracted from CEC or FFE cells in M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) with 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
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bioRxiv - Physiology 2022Quote: ... at which point the pellet was fixed with 2.5% (v/v) glutaraldehyde and 2.0% (v/v) paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.4 (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... at a 1.8030mg/mL (33μM) initial concentration and incubated overnight at 4°C under agitation with 1μL Alexa Fluor 488 C5-maleimide (Invitrogen) at a 20mM concentration in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphate buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Molecular Biology 2022Quote: ... The H/D exchange was quenched with additional ice-cold quenching buffer (400 mM KH2PO4/H3PO4, pH 2.2, 2.85 M GuHCl) and injected immediately into HPLC (UltiMate 3000, Thermo Fisher) with in-line peptic digestion and desalting steps ...
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bioRxiv - Genomics 2022Quote: Proteins were prepared from flash frozen cell pellets using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, Cat # 78503) following vendor’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and then the lavage was added to 200 μl of PBS with 50% FBS and 0.1 M EDTA (Gibco) and kept on ice ...
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bioRxiv - Physiology 2022Quote: Stock concentrations of IL-13 (1 μM; H2O with 0.1% (w/v) bovine serum albumin) and capsaicin (1 mM; 100% ethanol) were purchased from ThermoFisher and Sigma-Aldrich respectively and dissolved as described ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with 1 µM Rhonox-M for 1 h or lysotracker deep red (Thermo Fisher Scientific L12492) according to the manufacturer’s instructions for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Spheroplasts were washed with SP buffer and resuspended in SH buffer (0.6 M sorbitol, 20 mM HEPES-KOH [pH 7.4] containing protease inhibitor mixture (1861284; Thermo Scientific). Whole cell homogenates were subjected to centrifugation (500 × g ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed once using 200 µL/well of prewarmed 0.4 M sucrose solution in 1x PBS (diluted from 10x stock, #14200067, Gibco), followed by fixation for 15 min at room temperature using a solution consisting of 4% formaldehyde (Thermo Scientific ...