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Citations for Thermo Fisher :
4551 - 4600 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphat buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells lysates were incubated with antibody-conjugated Dynabeads or Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific) washed twice in ice-cold CLIP lysis buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA purification and enrichment of biotin-labeled DNA with Dynabeads™ M-280 streptavidin (Thermo Fisher Scientific, Cat# 11205D). The enriched bead-bound DNA was subjected to end repair ...
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... The band at the right size was cut out, sliced, and eluted in gel extraction buffer (0.5 M NH4Ac, 1 mM EDTA, 0.2% SDS, 1xRNAsecure™ (Invitrogen)) at 37ºC overnight ...
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bioRxiv - Systems Biology 2024Quote: ... and carotenes were extracted with glass beads and 500 μL of acetone in three 20 s rounds of agitation at 0.05 m/s with a FastPrep FP120 cell disruptor (ThermoFisher). The acetone phase was transferred to a new tube and the extraction was repeated twice ...
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bioRxiv - Biophysics 2023Quote: ... dark frames were acquired with the lasers turned off and camera shutters closed and z-scan calibration stacks of the 2-μm axial range DH-PSF in the green channel were acquired with fiducial beads (T7280, TetraSpeck, 0.2 μm, Invitrogen) spin coated in 1% (w/w ...
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bioRxiv - Biophysics 2023Quote: ... dark frames were acquired with the lasers turned off and camera shutters closed and z-scan calibration stacks of the 12-μm axial range DH-PSF in the red channel and the 2-μm axial range DH-PSF in the green channel were acquired with fiducial beads (T7280, TetraSpeck, 0.2 μm, Invitrogen) spin coated in 1% (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were identified using an ACQUITY M-Class UHPLC system (Waters) connected to an Orbitrap Elite mass spectrometer (ThermoFisher). Approximately 1 µg of samples was injected onto an ACUITY UPCL M-Class Symmetry C18 Trap Column ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Cell Biology 2023Quote: Blood was collected from hearts of freshly sacrificed mice to tubes coated with 0.5 M EDTA pH 8 (Invitrogen) to prevent clotting ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with 1.5 mg of affinity-purified sheep anti-mouse IgG antibodies covalently attached to superparamagnetic Dynabeads M-280 (Invitrogen) for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... Isolated mononuclear cells were washed three more times in a volume of 500 μL of HBSS containing 0.01 M HEPES (Gibco), using a microcentrifuge for 7 min at 600 × g at 4 °C.
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bioRxiv - Microbiology 2023Quote: ... Blood was collected by cardiac puncture using syringes coated internally with 50 μL of 0.5 M EDTA (Gibco, MA) then subjected to three sequential treatments with 1X RBC lysis buffer (Tonbo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... full-length molecular clones containing GFP and m-cherry reporters respectively 33,40,57 were transcribed in vitro using the mMESSAGE mMACHINE kit (Invitrogen, AM1344) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA libraries in the flowthrough were precipitated at −20°C for 18 hours in ethanol with 0.3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... kindly provided by MedImmune) were independently conjugated with 50 μL of M-280 Dynabeads (sheep anti-mouse IgG, Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... An autosampler and reverse phase UPLC chromatography column (2.6 µ m Accucore RP-MS, 100 mm length x 2.1 mm i.d.; ThermoFisher Scientific) was used to separate ABA with an acetonitrile (2%-55% ...
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bioRxiv - Genetics 2023Quote: ... The root (∼60 mg) was incubated for 2 h at 56°C with 950 μl 0.5 M EDTA pH 8.0 (Thermo Fisher), 50 μl 1% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The biotinylated proteins were incubated overnight with streptavidin-coupled Dynabeads (Dynabeads M-280 Streptavidin, Thermo Fisher Scientific, Waltham, MA) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of total RNA was reverse transcribed using random primers with M-MLC reverse transcriptase (Invitrogen, cat#28025021). Real-time PCR was conducted on Roche Lightcycler 96 Real time PCR system (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcriptase reactions were performed with oligo(dT)18 using the M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific). The cDNA samples were assayed in real-time quantitative PCR (qPCR ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS (0.1 M, pH 7.4) and incubated with rabbit anti-GFP (1:500; Invitrogen, A11122) and chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2023Quote: ... and protein from whole cell fraction as well as supernatants were extracted with M-PER buffer from Thermo Fisher Scientific (Cat ...
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bioRxiv - Plant Biology 2023Quote: ... for 30 min at 37 °C and then reversed transcribed with M-MLV Reverse Transcriptase (Invitrogen™, cat # 28025013). Real-time PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with 0.1 M PBS containing 0.3% triton X-100 then incubated in secondary antibodies (donkey anti-rabbit Alexa-fluor 594, Invitrogen A21207 and donkey anti chicken Alexa-fluor 647 ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng total RNA using a mix of M-MLV reverse transcriptase (Invitrogen, UK, #28025013), dNTP 10mM (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Gas chromatographic separation was conducted on a ThermoFisher Scientific Trace 1300 GC with a TraceGold TG-5SilMS column (0.25 μm film thickness; 0.25 mm ID; 30 m length; ThermoFisher Scientific), as described (Tompkins et al. ...
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bioRxiv - Biochemistry 2023Quote: ... carrying the RSV A2 WT or T205D M along with pcDNA3.1 codon-optimized plasmids carrying RSV A2 P and F using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in saline and transcardially perfused with 0.1 M phosphate-buffered saline (PBS, pH 7.4) followed by 10% neutral-buffered formalin (NBF, Fisher Scientific). After removing the fiber implants ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in TNB blocking buffer comprised of dH2O containing 0.1 M Tris-HCl (pH 7.5, Fisher Scientific), 0.15 M NaCl (Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 400 μg protein from each sample was reduced and alkylated with 10 mM TCEP (0.5 M stock, Thermo Scientific) and 20 mM IAM at 37 °C for 60 min in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... the Pt electrode sites were first cleaned by immersing them in 0.05 M H2SO4 (Fisher Scientific, Pittsburgh, PA, USA) and scanning from -0.35 V to 1.5 V at 1 V/s for 100 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... and generation of complementary DNA was mediated by M-MLV reverse transcriptase following the manufacturers’ instructions (ThermoFisher, Cat#28025021). Final samples were diluted to a volume of 200µL prior to RT-PCR analysis ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: ... 2 μg of anti-NSD2 antibody (ab75359) and 20 μl of Dynabeads M-280 sheep anti-mouse IgG (Invitrogen) were mixed in immunoprecipitation dilution buffer and incubated for 6 h at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... m/z and retention time values were exported into a TraceFinder compound data base (Version 4.1, Thermo Fisher Scientific). From this compound database we generated a TraceFinder method for each sample set and extracted the corresponding peaks from each full scan MS spectrum.
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bioRxiv - Synthetic Biology 2023Quote: HEK 293T cells were lysed 16 hours post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then cleared by centrifugation (>10000 rcf, 4°C, 30 m, Sorvall Legend XTR (Thermofisher, Waltham, MA)) and the decanted supernatant was quantified with a microplate Bradford assay per manufacturer’s instructions (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... complexes were trapped with MES buffer-preequilibrated streptavidin-coated magnetic beads (Dynabeads™ M- 280 Streptavidin, ThermoFisher Scientific, #11205D). Excess antibody was washed by incubating 3 × 15 min with MES buffer without BSA ...
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bioRxiv - Biophysics 2023Quote: ... dark frames were acquired with the lasers turned off and camera shutters closed and z-scan calibration stacks of the 12-μm axial range DH-PSF in the red channel and the 2-μm axial range DH-PSF in the green channel were acquired with fiducial beads (T7280, TetraSpeck, 0.2 μm, Invitrogen) spin coated in 1% (w/w ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... After one hour pre-clearance with 30 μL per ml washed (0.5 mg/mL BSA/PBS) magnetic Dynabeads M-280 (Invitrogen) at 4°C with rotation ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was reverse transcribed into cDNA using random hexameric primers and the M-MLV reverse transcriptase (ThermoFisher Scientific) by the following program ...
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bioRxiv - Synthetic Biology 2023Quote: ... BL21-AI [F-ompT hsdSB(r - m -) gal dcm araB::T7RNAP-tetA] was purchased commercially (ThermoFisher Scientific, MA, USA).
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed using either Superscript™ II or M-MLV reverse transcriptase according to manufacturer’s protocols (Invitrogen, Waltham ...
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bioRxiv - Plant Biology 2024Quote: ... split flow 6.0 mL/min) onto a TG-5SILMS GC Column (30 m × 0.25 mm × 0.25 µm; Thermo Fisher Scientific), using helium as a carrier gas at a constant flow of 1.2 mL/min ...
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bioRxiv - Developmental Biology 2024Quote: Human Umbilical Vein Endothelial Cells (HUVEC) (ATCC, Cat. #: PCS100010) were culture in 1X M-199 Medium (Gibco, Cat. #: 12340030) supplemented with 16 % FBS (Gibco ...
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bioRxiv - Neuroscience 2024Quote: Brains were dissected from 2-day old adults in PBS and incubated in 1.6 × 10-6 M acridine orange (ThermoFisher) for 5 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin conjugated EdU-labeled DNA was then pulled down using 5 μL of Dynabeads M-280 streptavidin (Invitrogen, 11205D). Beads were pre-washed three times with 1x Tween buffer (5 mM Tris HCl pH = 8 ...
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bioRxiv - Microbiology 2024Quote: ... The remainder of the lysate was incubated with 0.1 mL of Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen) previously conjugated with 10 ug of anti-HA antibody (Millipore ...