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Citations for Thermo Fisher :
4601 - 4650 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Indicated antibodies (anti-PKM2, TRIM21, Flag, Myc and IgG) were incubated separately with Dynabeads M-270 Epoxy (Thermo Scientific) on a roller at 37°C overnight to generate antibody-conjugated beads ...
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bioRxiv - Microbiology 2024Quote: ... The peptide spectra were recorded over the mass range of m/z 350–1,800 using the Xcalibur 4.1.50 system (Thermo Fisher Scientific). MS spectra were recorded repeatedly ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 12.5% filtered rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Immunology 2021Quote: ... rat or rabbit serum and then stained with one or more of the following: B220 (clone RA3-6B2, ThermoFisher), Lyve-1 (clone ALY7 ...
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bioRxiv - Cell Biology 2020Quote: ... at a dilution of 1:500 and secondary detection was performed using goat anti-rat Alexa 488 antibodies (Invitrogen). Fiber area was measured by using ImageJ software ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dye were used in this study: anti-rat E-cadherin (Invitrogen, 13-1900, 1:300), anti-rabbit cleaved caspase-3 (Cell signaling ...
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bioRxiv - Immunology 2021Quote: ... 13-0443-82) and 1:200 VE-cadherin-Alexa Fluor 647(rat anti-mouse, clone 11D4.1, Thermo Fisher, BDB562242) in 1.25% BSA in PBS for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-mouse and anti-rat antibody conjugated to Alexa Fluor 488 or Alexa Fluor 546 (1:100, Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: INS-1 E rat insulinoma cells were cultured in RPMI 1640 supplemented with 1% antibiotic-antimycotic (Gibco, 15240 – 062), 1% L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with Alexa-fluor 488/555/647 Donkey/ anti rabbit/goat/rat IgG antibodies (Invitrogen, 1:250) for 4h at RT in TNBT ...
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bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were obtained from pooled lungs of control and nitrofen-exposed rat fetuses by trypsinization (0.25% trypsin; Gibco). Fibroblasts were isolated as described [7,14,15] ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used were Alexa Fluor 555 goat anti-rat IgG (Thermo Fisher Scientific, A21434; diluted 1:500) and Alexa Fluor 488 F (ab’)2-Goat anti-Mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... A 300 µl 1:300 dilution of the secondary antibody (goat Cy3- or Cy5-conjugated anti-rat-IgG, Invitrogen) was added ...
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bioRxiv - Bioengineering 2019Quote: ... Plates were washed twice with PBS/5% FBS and incubated with anti-rat IgG2a (PE) (Thermofisher, 12-4817-82) or anti-histag (PE ...
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bioRxiv - Microbiology 2019Quote: ... for Western immunoblotting and rat anti-mouse IgG H+L conjugated with FITC for FACs analysis (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... appropriate Alexa Fluor 488/555/647-conjugated anti-rat/rabbit/mouse/goat/chicken secondary (1:250, Thermo Fisher Scientific) in donkey serum were incubated for 1.5 hours (hrs ...
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bioRxiv - Neuroscience 2020Quote: ... L4-6 ganglia were isolated from adult male Sprague Dawley rats (175-250 g) and dissociated with collagenase (Invitrogen). Cells were washed and then plated with DMEM/F12 (Cellgro ...
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bioRxiv - Plant Biology 2020Quote: ... sections were incubated for 1 h with the secondary antibody Alexa Fluor 546 goat anti-rat IgG (Life Technologies) diluted 1:1,000 in 0.1% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... To functionalize the nanoparticles with either RI7217 or a rat isotype IgG control (Thermo Fisher Scientific, Waltham, MA, USA), we prepared solutions of 8 mg/mL antibody in borate buffer (100 mM borate ...
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bioRxiv - Cell Biology 2019Quote: ... hippocampi were dissected from E18 Sprague-Dawley rat embryos or E17 C57BL/6 mice embryos and treated with 0.25% trypsin in HBSS (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and then coated for 30 min at 37°C with 0.25 mg/mL rat tail collagen I (Gibco A1048301) diluted in 20 mM sodium acetate (Sigma-Aldrich S2889) ...
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bioRxiv - Biochemistry 2020Quote: Primary rat astrocytes (gift, Prof. Dr. Morten Nielsen, University of Aarhus, Denmark) resuspended in astrocyte medium (DMEM Glutamax (Gibco), 10% FBS and 1% Pen-Strep ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of protrudin mRNA were analysed using pre-validated primer sequences (rat Protrudin, RN01766356_m1; cat no. 4331182; ThermoFisher Scientific). Q-PCR was performed on a LighCycler Q-PCR machine (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... P0 mouse or rat hippocampi were dissected in ice cold Hank’s Balanced Salt Solution (HBSS; Cat# GIBCO 14185-052) supplemented with 2.5mM HEPES pH7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... accession number NM_001001502.3) or rat αSyn (rαSyn; accession number NM_019169.2) sequences were performed with Lipofectamine® 2000 (Invitrogen, Thermo Fisher Scientific) and Opti-MEM® I Reduced Serum Medium (Opti-MEM from Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... accession number NM_001001502.3) or rat αSyn (rαSyn; accession number NM_019169.2) sequences were performed with Lipofectamine® 2000 (Invitrogen, Thermo Fisher Scientific) and Opti-MEM® I Reduced Serum Medium (Opti-MEM from Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were stably transfected into Fischer Rat Thyroid (FRT) cells in 6-well plates using lipofectamine LTX (Thermo Fisher) and OptiMEM (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: The immortalized rat embryo fibroblasts (REF52 cells) cell line [84] were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, 11965092) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... and goat anti-mouse or anti-rat Alexa Fluor 568 at a dilution of 1:500 (Thermo Fisher Scientific) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... antennae were washed 3x in 1X PBS-T (0.1%) and Alexa-488 goat anti-rat (Invitrogen A110066, 1:200) was added to the solution ...
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bioRxiv - Immunology 2021Quote: Mouse and Naked mole-rat sorted TCs and thymic tissue were used for RNA extraction by Trizol (Thermo Fisher). RNA was quantified using a NanoDrop One (Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Then MSCs were generated by flushing the rats’ femurs with sterile Dulbecco’s modified Eagle’s medium (DMEM, Thermo Scientific, USA) and were plated in culture flasks with DMEM containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were washed then incubated in secondary antibody solution (Goat anti-rat IgG-alexa555 conjugated, 1:500, Invitrogen, A48263) for 1h in 0.2% Triton x-100 and 2% NGS in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa 555-conjugated anti-rat and Alexa 488-conjugated anti-mouse secondary antibodies (Molecular Probes). DNA fibres were imaged in a LSM780 confocal microscope using a 64x oil objective and lengths of CldU and IdU tracks were measured using ImageJ software (http://fiji.sc/Fiji).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: Mouse and Naked mole-rat sorted TCs and thymic tissue were used for RNA extraction by Trizol (Thermo Fisher). RNA was quantified using a NanoDrop One (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... the embryos were incubated with secondary antibody Alexa Fluor 488-conjugated goat anti-rat (Thermo Fisher, Waltham, MA, USA) (1:500 dilution ...
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bioRxiv - Biophysics 2020Quote: RN46A cells (a rat serotonergic neuronal cell line) were cultured in Dulbecco’s modified Eagle’s media-F12 (1:1) (Gibco, USA) media supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: The entire rat SELENOP 3’UTR was cloned into the pcDNA3.1 vector using the TOPO-TA cloning kit (Invitrogen). 3 copies of the viral RNA MS2 sequence separated by random sequence were synthesized commercially from IDT technologies (Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with secondary antibodies (anti-rat-800, LI-COR, 1:10000; anti-rabbit-680, Invitrogen, 1:10000) for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Rat ASC were grown in the same medium than human ASC supplemented with 10% of fetal calf serum (Thermofisher). To manufacture the ASC-enriched gelatin patch ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were diluted as following for immunofluorescence: Alexa Fluor 555 goat anti-rat (1:500, no. A21434; Invitrogen), Alexa Fluor 488 donkey anti-mouse (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-rat or isotype-specific anti-mouse secondary antibodies conjugated to Alexa Fluor-488 or - 568 (Thermo Fisher Scientific) were used at 1:400 dilution ...
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bioRxiv - Genomics 2020Quote: ... Alexa fluor 488 and 594 conjugated secondary donkey α-rat or donkey α-rabbit antibodies (1:250 dilution; Invitrogen) were used for fluorescent detection ...
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bioRxiv - Neuroscience 2020Quote: ... anti-rat or anti-chicken IgG (1:1000 for cells, 1:400 for tissues, all from Thermo Fisher Scientific) for 1 h for cells or 2 h for tissues at room temperature and the nuclei were stained with Draq5 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: The rat insulinoma cells (INS-1, 832/3)54 were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with a rat p75NTR-EGFP* fusion constructs31 using FuGENE transfection reagents (Fisher scientific, Cat: PRE2311). EGFP* consist of a monomeric A207K EGFP mutant ...
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bioRxiv - Neuroscience 2022Quote: ... DRG were dissected from adult rats (10-12 months old) (L2-L5) and incubated with 0.5% collagenase (Gibco 17104019) at 37 °C for 1 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... The excised node region was exposed to DMEM supplemented with 75% rat serum and 0.2-µm-diameter fluorescent beads (505/515, F8811; Invitrogen), and the motion of the beads in the node cavity was monitored in planes of ∼10 µm above the surface of node pit cells.
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bioRxiv - Developmental Biology 2020Quote: ... Single-cell suspensions were incubated with a rat anti-CD49f antibody conjugated with phycoerythrin (ThermoFisher Scientific, clone NKI-GoH3), followed by anti-phycoerythrin magnetic microbeads and enriched using a column-magnetic separation procedure (Miltenyi Biotec) ...