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Citations for Thermo Fisher :
4401 - 4450 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... naked mole-rats were scruffed and injected intraperitoneally (IP) with 1.12 mg/kg FM 1-43 (Thermo Fisher). For the socially isolated condition ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies use for immunofluorescent detection were goat anti-rat secondary Alexa fluor 488 (ThermoFisher A-11006), goat anti-rabbit secondary Alexa fluor 568 (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... Cells were incubated in 10 μg/ml goat anti-Rat-IgG secondary antibody (Thermo Fisher Scientific SA5-10023) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rat AlexaFluor 488 and AlexaFluor 647 (A-11006 and A-21247, Thermo Fisher; RRID:AB_2534074 and RRID:AB_141778). Brightness/contrast for each channel were scaled identically within each immunofluorescence experiment shown.
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bioRxiv - Cell Biology 2023Quote: ... followed by B220+ or CD19+ B-cells depletion with specific antibodies and anti-rat magnetic beads (Invitrogen 11417D). 2×106 of the remaining cells were stimulated with 2×106 anti-CD3/CD28 beads (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-SYCP3 antibody (1:1,000) (24) follower by applying the Alexa fluorescence-labeled secondary antibodies (Thermo Fisher). PNA-Cy5 (Vector laboratories ...
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bioRxiv - Cell Biology 2023Quote: Rat Basophilic Leukemia (RBL-2H3) cells were cultured as monolayer in MEM growth medium (Life Technologies, Carlsbad, CA) supplemented with 20% heat-inactivated Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-rat/hamster compensation bead kit and the ArC amine reactive compensation bead kit (Life Technologies, A10628) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies that were used are: Donkey Anti-Rat Alexa Fluor 488 (1:250, Cat: A21208, Invitrogen), Donkey Anti-Rabbit Alexa Fluor 555 (1:250 ...
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 647 (Thermo Fisher, catalog no. A21247, 1:200). GC reactions induced by M2ex3 trimers and SApNPs were studied by immunostaining ...
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bioRxiv - Cell Biology 2023Quote: ... AlexaFluor 647 goat anti-sheep IgG (H+L) and AlexaFluor 633 goat anti-rat IgG (H+L) (Invitrogen); IRDye 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit (LI-COR).
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bioRxiv - Genomics 2023Quote: ... Secondary antibodies: goat anti-mouse Alexa-633 and goat anti-rat Cy5 were used at 1:1000 (Invitrogen). Tissue was mounted in VECTASHIELD (Vector Labs ...
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bioRxiv - Neuroscience 2023Quote: ... the dishes were further coated with 30 μl rat anti-mouse CD11b antibody (ThermoFisher Scientific, 14-0112-82) diluted in the same buffer overnight at 4 °C and washed 5 times in PBS before use ...
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bioRxiv - Microbiology 2023Quote: ... into single cells and plated on Transwell membranes (StemCell) coated with rat tail collagen type I (Fisher Scientific) in Pneumacult-ALI medium (StemCell ...
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bioRxiv - Genetics 2023Quote: ... The following secondary antibodies were used at 1:200 dilutions: anti-rat Alexa Fluor Plus 488 (ThermoFisher A48262), anti-rabbit Alexa Fluor 555 (ThermoFisher A21428 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were thawed and seeded as a co-culture with 15% rat primary cortical astrocytes also from ThermoFisher Scientific (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were stained overnight in 4 °C with rat-anti-CD4 (1:100, Invitrogen, 14-9766-82) and rabbit-anti-Iba1 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies against rabbit or rat were conjugated to Alexa Fluor 488 or 647 (Molecular Probes, 1:500). DAPI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Secondary antibodies and concentrations used were: goat anti-rat conjugated with AlexaFluor 546 (Invitrogen A-11081, 1:300), and goat anti-rabbit conjugated with AlexaFluor 647 (Invitrogen A-21245 ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 2.0 × 104 purified PHH cells/well were seeded in rat tail collagen (collagen type I) (Life Technologies) coated 96-well plate and incubated in hepatocyte Incubation medium at 37 °C and 5% CO2.52 Twenty-four hours after incubation ...
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bioRxiv - Physiology 2024Quote: ... 6 rat samples (ExpA, B and C) were placed directly in Leibovitz’s L-15 medium (Gibco 11415-064) to develop organoids immediately ...
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bioRxiv - Biochemistry 2023Quote: ... eFluor™ 450 rat anti-mouse TER-119 clone TER-119 (ThermoFisher, 48-5921-82; 1:100 dilution), APC Mouse Anti-Mouse CD36 clone CRF D-2712 (BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... Isotype-specific anti-mouse or anti-rat secondary antibodies conjugated to AlexaFluor–488 or –568 (Thermo Fisher Scientific) were used at a concentration of 1:400 ...
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bioRxiv - Cancer Biology 2024Quote: ... and a rat anti-CldU monoclonal antibody (Accurate) followed by Alexa Fluor 568-conjugated goat anti-mouse (Invitrogen) and Alexa Fluor 488-conjugated goat anti-rat secondary antibodies (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and Alexa-Fluor-633-labelled goat anti-rat antibody (A-21094, Thermo Fisher Scientific) for 2 hours in the dark and at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... The secondary antibodies were Alexa Fluor 488 donkey anti-Rat IgG (H+L) (Thermo Fisher, A21208; 1:500) and Alexa Fluor 555 goat anti-Mouse IgG (H+L ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 4 ℃) with primary IgG antibodies: rat anti-Ki67 (14-5698-80, SolA15, 1:400, Invitrogen, Carlsbad, CA, USA), rat anti-BrdU (ab6326 ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor Plus 488 Goat anti-Rat IgG (H+L) (for SST stain; Catalog # A48262, Invitrogen; RRID: AB_2896330), and (2 ...
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bioRxiv - Biophysics 2021Quote: We previously cloned the genes for TEM and CTX-M-9 β-lactamase into pET24-b plasmids (Life Technologies) for inducible protein expression under the T7 promoter.[21 ...
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bioRxiv - Biochemistry 2020Quote: ... The extracted FFAs were then determined using a Thermo Scientific TRACE 1300 gas chromatograph (GC) equipped with a TG-WaxMS A column (30 m × 0.32 mm × 0.25 µm; Thermo Scientific) and a Flame Ionization Detector (FID ...
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bioRxiv - Biophysics 2020Quote: ... Protein concentration was determined using an ε280 = 12,700 M-1cm-1 and a molecular weight of 11.97 kDa on a NanoDrop 1000 (Thermo Scientific). S6 was aliquoted into 1 mL fractions of 1 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was performed on 2 μg of total RNA using M-MLV Reverse transcriptase and random hexamers (Invitrogen). For PCR reactions ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were resuspended in mammalian protein extraction reagent (M-PER, Pierce/Thermo Fisher Scientific, cat. no. 78503) containing protease inhibitor (ApexBio Technology ...
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bioRxiv - Cell Biology 2020Quote: ... Fragmentation was inhibited by the addition of 280 μL of Stop Solution (3 M NaOAc pH 5.5, 15 mg/mL GlycoBlue (Invitrogen)) ...
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bioRxiv - Cell Biology 2020Quote: ... the Fibrinogen anchor in the above protocol was replaced by the protein of interest at a concentration of 50 μg/ml in 0.1 M sodium bicarbonate pH 8.3 (except for fluorescent EGF, where biotinylated EGF complexed to streptavidin-Alexa555 (Invitrogen, E35350) was pattered at 1 μg/ml in 0.1 M sodium bicarbonate ...
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bioRxiv - Immunology 2021Quote: Tissues homogenates were prepared by homogenizing perfused lung using gentleMACS dissociator (Miltenyi) with the RNA_02 program in M tubes in 1 ml of Trizol (Invitrogen). The homogenates were centrifuged at 2000 g for 1 min at 4°C and the supernatant was collected ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Immunology 2021Quote: ... #130-096-427) with program RNA_02 in M tubes (#130-096-335) in 1 ml of Trizol (Invitrogen, #15596018). The homogenates were centrifuged at 2000 g for 1 min at 4°C and the supernatant was collected ...
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bioRxiv - Developmental Biology 2020Quote: ... were isolated from the embryos and cultured using a Durapore Membrane Filter 0.65 μm (#DVPP02500) placed on a stainless-steel mesh for up to 7 days in Ham’s F-12/DMEM (Gibco) supplemented with Insulin-Transferrin-Sodium Selenite Supplement (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-M-B436 and RKO were cultured in Dulbecco’s Modified Eagle Medium (DMEM) + GlutaMax (Thermo Fisher Scientific; Waltham, MA), supplemented with 10% FBS and 1% Penicillin/Streptomycin.
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed once with ice-cold PBS and lysed with RIPA/M-PER buffer (1:1; Thermo Fisher). Lysates were incubated on ice for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 ng of end-labeled pNG1175 DNA was mixed with 1 µL streptavidin-functionalized paramagnetic beads (M-270 Dynabeads, diluted 1:6 in 1X PBS with 0.1% BSA; Invitrogen) in 10 µL PBS ...
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bioRxiv - Immunology 2022Quote: Cells and mouse tissues were lysed in M-PER or T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific) containing additional 1X PhosStop (Sigma ...
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bioRxiv - Genomics 2020Quote: ... Labelled RNA was subjected to 1 round of affinity purification with streptavidin paramagnetic beads (Dynabeads M-280 Streptavidin, Invitrogen). Purified labelled RNA was quantified with Qubit (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... A total of 1 mg cytoplasmic protein was incubated with 100 μL Dynabeads M-280 streptavidin (Thermo Fisher Scientific) conjugated with 5′-biotinylated siRNA or 100 bp linearized DNA ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was incubated at 4 °C for 30 min with 60 μl of Dynabeads M-270 Streptavidin (Invitrogen) equilibrated with lysis buffer containing 1% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged at 13,000 rpm for 10 minutes and the supernatants were incubated with Dynabeads M-280 (Invitrogen) which were pre-blocked in buffer containing 20 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... Nucleosome concentrations were determined using an A260 extinction coefficient of 2,784,500 M−1 cm−1 on a Nanodrop spectrophotometer (Thermo Fisher).
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bioRxiv - Pathology 2019Quote: ... 1 μg of total RNA samples were reverse transcribed using the M-MLV-RT system (Invitrogen™, Shanghai, China). The program was performed at 42 °C for 1 h and terminated by deactivation of the enzyme at 70 °C for 10 min ...