Labshake search
Citations for Thermo Fisher :
4451 - 4500 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: The monocytic cell line U937 (obtained from Dr. M. Servant, Université de Montréal, Quebec, Canada) was cultured in RPMI medium (GIBCO) supplemented with 1% L-glutamine (GIBCO) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The pooled samples were incubated with 100 µl pre-equilibrated magnetic IgG-coupled Dynabeads M-270 Epoxy (Life Technologies) for 1 h at 4 °C ...
-
bioRxiv - Cancer Biology 2019Quote: ... DiI-VLDLs were dialyzed into PBS containing 0.05 M sodium borate (pH 8.5) and incubated with DyLight 650 NHS-Ester (ThermoFisher Scientific) at room temperature for 1.5 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested by addition of 100 µl of 0.05 M ABC containing 0.3 µg of trypsin (90057, Thermo Scientific) at 37° C for 16 hours ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.25 M cell equivalents were run on each lane of a 7% NuPage Tris-Acetate gel (Invitrogen, Carlsbad, CA) and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore ...
-
bioRxiv - Genomics 2019Quote: ... BS(PEG)9 was then aspirated and quenched by incubating in 1 M Tris-HCl pH 8.0 (AM9855G, ThermoFisher) for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... the primary microglia and M-MØP cells were then imaged using an EVOS FL Auto 2 onstage incubator system (Thermofisher). Images at x40 from cells in the 8 well chamber slide were taken every 30 minutes until the 2 hour mark ...
-
bioRxiv - Synthetic Biology 2021Quote: ... One was an Orbitrap mass spectrometer (positive mode, scan range of 200–1,500 m/z, 60,000 FWHM resolution, LTQ Orbitrap Velos Pro, Thermo Scientific), which was used for a high-resolution full-scan analysis ...
-
bioRxiv - Microbiology 2021Quote: ... hippocampi were washed three times in HBSS (HBSS, 1-M HEPES, penicillin/streptomycin, and 100-mM sodium pyruvate, Gibco) and were dissociated via trypsin digestion (2.5% ...
-
bioRxiv - Bioengineering 2020Quote: ... 1×1010 sEV were incubated with 3.8 µm aldehyde/sulfate latex 4% (m/v) beads (Molecular probes, Eugene, OR). Coated beads were then incubated with anti-human CD63 (eBioscience ...
-
bioRxiv - Biochemistry 2020Quote: ... Stage tips were generated with two plugs of 3 M™ Empore™ SDB-XC Extraction Disks (Fisher Scientific) that were activated with 100% acetonitrile (ACN ...
-
bioRxiv - Neuroscience 2020Quote: ... mDANs were stained for live-cell imaging by using 1:10000 MitoTracker Green FM (M-7514, Thermo Fisher Scientific) to label mitochondria and 1:5000 LysoTracker Deep Red (L-12492 ...
-
bioRxiv - Plant Biology 2021Quote: ... Separation was accomplished with a 30 m TG-5MS column (Thermo Scientific, 0.25 mm i.d., 0.25 μm film thickness) and a helium gas at 1.2 mL/min flow rate ...
-
bioRxiv - Plant Biology 2020Quote: ... Metabolites were separated with a 30 m TG-5MS column (Thermo Scientific, 0.25 mm i.d. 0.25 μm film thickness). The GC program began at 80 °C for 0.5 min and ramped to 330 °C at a rate of 15 °C per minute and ended with an 8 min hold at a 1 mL · min-1 helium gas flow rate ...
-
bioRxiv - Systems Biology 2021Quote: ... Metabolites were separated with a 30-m TG-5MS column (Thermo Scientific, 0.25 mm i.d. 0.25 μm film thickness). The GC program began at 80 °C for 0.5 min and ramped to 330 °C at a rate of 15 °C per minute and ended with an 8 min hold at a 1.2 mL · min−1 helium gas flow rate ...
-
bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
-
bioRxiv - Genetics 2021Quote: ... overnight at 4°C and then for 2 hours with Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen 11201D). After 3 800uL washes in LiCl buffer (100mM Tris HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... 21.56ul Glycerol, 21.3ul 2X Dialysis buffer (100 mM HEPES-KOH pH 7.2, 0.2 M NaCl(Thermo Fisher Scientific, AM9760G), 0.2 mM EDTA(Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... a mixture of 10% 10,000 MW BDA and 3% 3,000 MW BDA in 0.01 M sodium phosphate buffer; Molecular Probes, Invitrogen) was injected ...
-
bioRxiv - Immunology 2021Quote: ... Blood was extracted from the vena cava and mixed with 100 μL of 0.5 M EDTA (Thermo Fisher Scientific) prior to red blood cell lysis using an ACK lysis solution (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... First-strand complementary DNA synthesis was performed with the RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific). Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex ...
-
bioRxiv - Molecular Biology 2021Quote: ... PolyA RNA was ethanol precipitated with 2.5 M Ammonium Acetate and 70% ethanol in a solution containing 50 μg/ml GlycoBlue Co-precipitant (AM9515, Invitrogen). RNA was resuspended in 10 μl and fragmented with 10x Fragmentation Buffer (AM8740 ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesised from equal amount of RNA by reverse transcription using M-MLV reverse transcriptase (Thermo Fisher Scientific) and a mixture of random primers (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... the lysate was clarified by centrifugation at 17K x g and the supernatant was batch-incubated for 45 min at 4 °C with 3 mL of Ni-NTA agarose resin that had been pre-treated with 0.5 M imidazole pH 7.4 followed by equilibration with lysis buffer (ThermoFisher Scientific). The protein-bound resin was passed over a column and washed with 20 column volumes of Wash10 buffer [100 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... NIH3T3 or HeLa cells were lysed with 100 μL M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) for 10 min following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA was recovered by precipitation with isopropanol in presence of 0.3 M sodium acetate pH 5.5 and 20 μg Glycoblue (Thermo Scientific). Recovered RNA was dephosphorylated with T4 PNK (NEB ...
-
bioRxiv - Immunology 2022Quote: ... CR3022-F(ab’)2 was buffer exchanged into the prepared 0.1 M sodium phosphate using Zeba columns (ThermoFisher, USA). DOTA-NHS-ester (#B-280 ...
-
bioRxiv - Cell Biology 2022Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (ThermoFisher); after 72 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 μL of the diluted solution was mixed with 20 μL of Dynabeads M-280 streptavidin (Thermo Fisher Scientific) at 25°C for 5 min ...
-
bioRxiv - Microbiology 2022Quote: ... Isolated mononuclear cells were washed three more times in a volume of 500 μL of HBSS containing 0.01 M HEPES (Gibco), using a micro-centrifuge for 7 min at 600 × g ...
-
bioRxiv - Genetics 2022Quote: 1 µg DNase-treated RNA from each sample was reverse-transcribed to cDNA using M-MLV Reverse Transcriptase (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... The bacteria were then centrifuged and resuspended in Sodium carbonate buffer (0.1 M, pH 9.0) for an additional staining step with the pH-sensitive dye CypHer5E (Fisher scientific). This was used at a concentration of 7 μg/ml in a volume of 1.5 ml for 2h at room temperature under gentle rotation ...
-
bioRxiv - Cell Biology 2022Quote: ... The resuspension was clarified by centrifugation at 16,000 g for 10 min at 4 °C The clarified protein lysate was mixed with 1.5 mg magnetic beads (Dynabead M-270 Epoxy, ThermoFisher) conjugated with in-house GFP nanobody (Fridy et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was harvested and a ¼ volume of a 40% polyethylene glycol (PEG 8000; BioChemica) and 1.915 M NaCl (ThermoFisher) solution was added prior to incubation for 3 h on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... 90% of each cell lysate was centrifuged at 13,000 rpm for 5 minutes and the supernatants were transferred and incubated with streptavidin beads (Dynabeads M-280, Invitrogen), which were already blocked by 0.2% Chicken Egg Albumine (Sigma) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Slides were neutralized in 0.4 M Tris pH 7.4 and stained with 50 µL of GelRed (Thermo Fisher Scientific). Samples were examined with a Leica DMI 3000B microscope (Germany) ...
-
bioRxiv - Microbiology 2021Quote: ... First-strand complementary DNA synthesis was performed with the RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific). Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex ...
-
bioRxiv - Cell Biology 2020Quote: ... Two microgram RNA was reverse transcribed into cDNA using random primers and M-MLV reverse transcriptase (Invitrogen, CA, USA). RT-qPCR was performed in triplicate with the Eco Real-Time PCR System (Illumina ...
-
bioRxiv - Neuroscience 2020Quote: ... and the samples were first post-fixed overnight (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 days for decalcification ...
-
bioRxiv - Neuroscience 2020Quote: ... of brain grey matter tissue was homogenized in 500 μL of 8 M urea lysis buffer (8 M urea, 100 mM Na2HPO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher) using a Bullet Blender (NextAdvance) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The hybridized DNA fragments were enriched with streptavidin-attached magnet beads (Dynabeads M-270, Thermo Fisher Scientific, Markham, ON) and washed under increasing stringency to remove non-targeted DNA sequences (xGen® Lockdown® Reagents ...
-
bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
-
bioRxiv - Neuroscience 2020Quote: ... the sections were washed in 0.1 M TB (3 ×5 minutes) and then incubated in goat anti-chicken Alexa 488 (1:1000, #A11039, Invitrogen), goat anti-rabbit Alexa 568 (1:500 to 1:1000 ...
-
bioRxiv - Bioengineering 2021Quote: ... was dissolved under stirring in 15 mL of an alginate solution (10 mg/mL-1) formed in 0.1 M MES buffer at pH 6 (ThermoFisher). Sulfo-NHS (41 mg ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dynabeads® Co-immunoprecipitation Kit including Dynabeads® M-270 Epoxy beads were used (Thermo Fisher Scientific, MA, USA). 5mg of beads/IP were conjugated with respective antibodies (FXR1 Proteintech ...
-
bioRxiv - Neuroscience 2022Quote: ... 200 ng of RNA was taken and cDNA synthesis was done with random hexamer and while doing the reverse transcription enzyme (M-MLV cat.no 28025013, Invitrogen) was excluded (minus reverse transcription ...
-
bioRxiv - Molecular Biology 2022Quote: ... inlet into a Thermo Trace gas chromatograph fitted with a Trace TR-50MS GC column (60 m × 0.25 mm, 0.25 μm film thickness, Thermo Fisher). Analytes were separated with helium as the carrier gas at a constant flow of 1.2 ml/min with vacuum compensation ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2 uL of RNA from each fraction were reverse-transcribed using 20U of M-MuLV Reverse Transcriptase (Thermo Scientific) and 0.3 ug of random hexamer primers in a reaction volume of 20 uL ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Each buffer mixture was then mixed with SeaKem®GTG agarose (2% m/v; Life Technologies, Gaithersburg, MD, USA) and gelled in a Petri dish so that the thickness of the gel layer was about 1 mm ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from equal amount of RNA by reverse transcription using M-MLV reverse transcriptase (Thermo Fisher Scientific) and a mixture of random primers (Thermo Fisher Scientific) ...