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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and then treated with Alexa Fluor 488-conjugated goat anti-human IgG (catalogue no. A11013; Thermo Fisher Scientific) at a concentration of 2 μg/ml in DMEM with 2% FBS for 30 minutes (min ...
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bioRxiv - Microbiology 2024Quote: ... quantified using the QubitTM dsDNA BR Assay Kit (Q32850) and a Qubit spectrofluorometer (Qubit 3.0, Thermo Fisher Scientific, USA), and submitted for complete sequencing (Plasmidsaurus Inc. ...
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bioRxiv - Microbiology 2024Quote: ... Fixed parasites were stained with SYBR Green (Thermo Fisher Scientific, 1:10,000 dilution) for 20 min at 37°C and analysed by flow cytometry on a BD FACSVerse using BD FACSuite software ...
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bioRxiv - Microbiology 2024Quote: ... followed by AlexaFluor 647-conjugated anti-human antibodies (Invitrogen, 1:1,000). Slides were then stained with 1 μg/mL DAPI and mounted in Citifluor (Citifluor Ltd. ...
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bioRxiv - Microbiology 2024Quote: ... the quantity and quality of each DNA sample were assessed using a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). For DNA extraction from the inner part of plant roots (endosphere) ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 500 µL of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) (Thermo Scientific, TS48915) and heated for 1 h at 70 °C ...
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bioRxiv - Microbiology 2024Quote: ... CHOs were transfected using Lipofectamine 3000 transfection reagent (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Trypsin digestion was performed overnight at 37 °C under constant shaking at 600 rpm using an Eppendorf Thermomixer (Thermo Scientific). Proteins were digested a second time using the same protein to trypsin ratio of before ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Microbiology 2024Quote: ... cells were treated with 0.25% trypsin-EDTA (Gibco, Cat# 25200-056) or Accutase® solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: Protein concentration was measured using a NanoDrop™ OneC instrument (Thermo Scientific). Each sample was adjusted to 10 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... and 2mM copper (II) sulfate pentahydrate all dissolved in UltraPure distilled water (Invitrogen) (90) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were grown in Ham’s F-12K medium (ATCC) with HI-FBS and 1x Antibiotic-Antimycotic (Gibco) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Oral administration of the phage preparation was perform as described (31) using disposable animal feeding needles (20G × 1.50 in.; Thermo Fisher Scientific, location!!). The volumes of each dose of YPP-401 for various routes were ...
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bioRxiv - Microbiology 2024Quote: ... The dried extracts were silylated to produce trimethysilyl (TMS) derivatives by dissolving in 500 µL of silylation grade acetonitrile (Thermo Scientific, TS20062), followed by addition of 500 µL of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA ...
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bioRxiv - Genomics 2024Quote: ... 1% agarose gel and Qubit (dsDNA BR Assay, ThermoFisher).
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bioRxiv - Microbiology 2024Quote: ... and 50 µL spots were pipetted onto BHIS agar plates supplemented with 11 mM glucose (ThermoFisher Scientific) and 4 µg/mL Amp ...
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bioRxiv - Microbiology 2024Quote: ... and 1% penicillin/streptomycin (P/S; Gibco). Cells were maintained by diluting with fresh medium every 2-3 days ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strains were grown in Luria-Bertani (LB; ThermoFisher Scientific) medium at 37 °C in a MaxQ 6000 incubator (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis was performed using an UltiMate® 3000 nano splitless reversed-phase nanoHPLC (Thermo Fisher Scientific, Dreieich) coupled online to a timsTOF™ pro mass spectrometer (Bruker Daltonik GmbH ...
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bioRxiv - Microbiology 2024Quote: ... The DNA concentration was quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, Delaware, USA). A partial 16S rRNA gene sequence and a whole 16S-23S ITS sequence were amplified by PCR using the primers P2 (5’- GGGGAATTTCCGCAATGGG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... into sterile 96-well plates (Fisher Scientific). The plates were incubated at 37 °C for 24 h in a Stratus microplate reader (Stellar Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... After removing rRNA by using the MICROBExpress kit (Ambion), mRNA was used to generate the cDNA library according to the NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... and 1% penicillin-streptomycin (Gibco™) at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2024Quote: ... and 3% amphotericin B (Gibco)) ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg/mL thiamphenicol (Tm) (ThermoFisher Scientific), 100 µg/mL kanamycin (Kan ...
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bioRxiv - Microbiology 2024Quote: ... Quality control of amplified products was achieved by running a high-throughput Invitrogen 96-well-E-gel (Thermo Fisher Scientific, Waltham, MA, USA). PCR products from the same samples were pooled in one plate and normalised with the high-throughput Invitrogen SequalPrep 96-well Plate kit ...
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bioRxiv - Microbiology 2024Quote: ... medium at 37 °C in a MaxQ 6000 incubator (ThermoFisher Scientific). Bacterial strains that carried plasmids were maintained using the following antibiotics at the indicated concentrations ...
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bioRxiv - Microbiology 2024Quote: ... Vero E6 cells were grown in monolayers in Minimum Essential Medium (MEM, Gibco™) complemented with 7% heat-inactivated Fetal Bovin Serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... 1% L-glutamine (Gibco™) and 1% penicillin-streptomycin (Gibco™ ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of culture supernatant was collected and treated with 2 µl (5 U) DNase solution I (ThermoFisher Scientific) at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... The qRT-PCR program ran on QuantStudio 3 Real Time PCR System (Thermo Fisher), with the temperature profile in accordance with the Luna Universal qPCR Master Mix instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration was then quantified with the QubitTM RNA BR Assay kit (Thermo Fisher Scientific) on the Qubit Flex Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The gel was stained with 1x SYBR gold dye (Thermo Fisher Scientific GmbH, Dreieich) and imaged with AlphaImagerTM (Biozym Scientific GmbH ...
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bioRxiv - Microbiology 2024Quote: ... The transformants were selected on solid LB (Sigma, Fisher Scientific) medium (agar 15% ...
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bioRxiv - Microbiology 2024Quote: ... complementary DNA (cDNA) was synthesized from 200 ng RNA templates using the Maxima H Minus Reverse Transcriptase kit (Thermo Fisher) following the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... frozen erythrocytes were melted and directly used as template (Phusion Blood direct PCR kit, Fisher Scientific) as described previously (Zamoto-Niikura 2020) ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were purified using Ni-NTA (Thermo Fisher™) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... biofilms were rinsed in PBS then stained with a concanavalin-A conjugated to Alexa 647 (Invitrogen) (50 µg/ml ...
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bioRxiv - Microbiology 2024Quote: Reverse transcription quantitative PCR analysis was performed on QuantStudio 5 Real-Time PCR system (Thermo Scientific, USA) using the primer set from E or RdRp Charité (IDT Coralville ...
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bioRxiv - Microbiology 2024Quote: ... Analysis was conducted with Ultimate 3000 nano RSLC system coupled to Orbitrap Exploris 480 mass spectrometer (both Thermo Fisher Scientific, Waltham, MA, USA) with FAIMS ...
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bioRxiv - Microbiology 2024Quote: ... Trx-Forward and pBAD-Reverse (Thermo Fisher Scientific). As a control ...
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bioRxiv - Microbiology 2024Quote: ... Up to 10 µg of total RNA was afterwards treated with 2 U of DNase I endonuclease (Thermo Fisher) for 30-60 min at 37°C in a volume of 20 µL ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were transformed into TOP10 competent cells (Thermo Fisher Scientific). Transformants were screened by colony PCR using Bd37 internal primer Bd37-SQF1 (supplement table1 ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted in 1 ml of TRI Reagent™ Solution (Ambion, Invitrogen; Carlsbad, CA) using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... GenBank accession number: MN908947.3) were cloned into a pcDNA3.1 expression vector (V79020, ThermoFisher) in frame with an N-terminal V5-tag ...
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bioRxiv - Microbiology 2024Quote: DMEM without glucose was either purchased (Gibco) or prepared using DMEM powder (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were stripped in boiling 0.1% SDS for 10 minutes then re-probed overnight 32P-end-labeled primer “5S.” The ladder was prepared using the Decade Markers System from ThermoFisher, according to manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 15-20μg of total protein was loaded onto precast 4-12% iBlot2 Bis-Tris gels (ThermoFisher Scientific) and allowed to run for ∼90 minutes at 120V ...