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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... RC Dialysis tubing (3.5 kDa or 8 kDa molecular weight cut-off, Spectrum Spectra/Por 6, Fisher Scientific), HCl (2M ...
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bioRxiv - Bioengineering 2024Quote: ... and the resulting supernatant was assayed using a human albumin ELISA kit (ThermoFisher Scientific; EHALB) according to the manufacturer’s instruction.
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bioRxiv - Bioengineering 2024Quote: ... Ethidium Homodimer-1 (Life Technologies, 4.0 mM), Hoechst (1 mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Tween 20 (ThermoFisher), DAPI (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... bovine serum albumin (BSA, Fisher Scientific), Rhodamine Phalloidin (Cytoskeleton ...
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bioRxiv - Bioengineering 2024Quote: ... Coupling 5-(and 6)-Carboxytetramethylrhodamine (TAMRA) mixed isomers (Thermo Scientific, Waltham, MA) and Fmoc-8-amino-3,6-dioxaoctanoic acid (Fmoc-PEG2-OH ...
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bioRxiv - Bioengineering 2024Quote: ... The supernatant was then ultracentrifuged in PET Thin-Walled ultracentrifuge tubes (Thermo Scientific 75000471) at 12,000g with a Sorvall WX+ Ultracentrifuge equipped with an AH-629 rotor (k factor = 242.0 ...
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bioRxiv - Bioengineering 2024Quote: ... were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass, Thermo Scientific) and cultured for 3 days before imaging[46–48] ...
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bioRxiv - Bioengineering 2024Quote: ... The CEPT cocktail consists of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Bioengineering 2024Quote: ... The frozen organoids were sectioned at 8 µm-thick slices on a Leica CM3050S cryostat (-18°C chamber, - 20°C object, 3.5° blade angle) and mounted to silanated microscope slides (Superfrost Plus, Fisher Scientific, Hampton, NH). The slides with sectioned organoids were dried under laminar flow at room temperature and kept at -80°C until staining.
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bioRxiv - Bioengineering 2024Quote: The viability of dynamically and statically cultured cerebral organoids on day 31 was measured with the CellTiter-Glo 3D® cell viability assay kit as well as a fluorescence-based Live/Dead™ viability/cytotoxicity kit (L3224, Life Technologies) containing calcein AM and ethidium homodimer-1 (EthD-1 ...
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bioRxiv - Bioengineering 2024Quote: ... 1% (v/v) N2 supplement (17502001, ThermoFisher), 1% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... Real time PCR was performed using SYBR™ Green Master Mix (A25742, ThermoFisher) and forward/reverse primers from IDT Technology ...
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bioRxiv - Bioengineering 2024Quote: ... 3% hESC quality FBS (10439001, ThermoFisher), 100 µM 2-mercaptoethanol (21-985-023 ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of cDNA conversion kit (4368814, Applied Biosystems). Real time PCR was performed using SYBR™ Green Master Mix (A25742 ...
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bioRxiv - Bioengineering 2024Quote: ... Endotoxin removal was performed using Pierce High-Capacity Endotoxin Removal resin (Thermofisher) and validated using the Pierce LAL Chromogenic Endotoxin Kit Quantitation Kit (Thermofisher).
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bioRxiv - Bioengineering 2024Quote: ... 1% (v/v) B27 supplement without vitamin A (12587010, ThermoFisher), 0.025% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... in DPBS (Gibco). Bones were then sterilized using 70% ethanol for 3 mins ...
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bioRxiv - Bioengineering 2024Quote: ... in DPBS (Gibco). Single cell suspension was prepared by meshing spleen against 100 μm strainer ...
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bioRxiv - Bioengineering 2024Quote: ... Buffer was made by 200-fold dilution of Quant-iT PicoGreen dsDNA Reagent (ThermoFisher, P11495) in 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were rinsed with PBS and mounted in the dark with Fluoromount-G (Invitrogen, Waltham, MA). Sections were imaged using an EVOS M5000 microscope (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... The isolated cells were then activated with magnetic anti-human CD3/CD28 dynabeads (CTS, ThermoFisher). Beads were mixed at a 1:1 ratio with cells and cells were cultured at a density of 1 x 106 cells/mL in complete X-Vivo 15 culture media (Lonza ...
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bioRxiv - Bioengineering 2024Quote: DNA content was quantified using a standard DNA assay kit (Quant-iT™ PicoGreen dsDNA kit; Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Cell concentration was quantified using a Countess II FL (Invitrogen) and each sample was diluted to 2*107 cells per mL ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were imaged using an EVOS M5000 microscope (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... Slides then stained with secondary antibody (1:500, Invitrogen, A11034) and 4’ ...
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bioRxiv - Bioengineering 2024Quote: Slides containing LN sections were first blocked using SuperBlock Buffer (Thermo Fisher Scientific) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Herceptin was buffer exchanged in PBS by using 7 K MWCO Zeba Spin Desalting Columns (ThermoFisher Scientific). Then it was incubated with 20ME (Molar equivalent ...
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bioRxiv - Bioengineering 2024Quote: ... and trypsin-EDTA (0.25%) from Gibco. No additional sterilization or treatment is required because all the substances were of good scientific quality.
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bioRxiv - Bioengineering 2024Quote: ... The scaffolds were mounted on stubs using a carbon tape and imaged using an SEM (Phenom Pro X, Thermo Scientific, MA, USA).
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bioRxiv - Bioengineering 2024Quote: ... Gene expression analysis was performed on Applied Biosystems real-time PCR system using SYBR Green Reaction Mix (Applied Biosystems, Cat # 4385612). Relative expressions of aggrecan (ACAN) ...
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bioRxiv - Bioengineering 2024Quote: ... and the cell pellets were resuspended in 50 µL Flow Cytometry Staining Buffer (Invitrogen). Cell concentration was quantified using a Countess II FL (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: Harvested spleens were placed into a prewetted 100 µm cell strainer (Fisher Scientific, Hampton, NH) in a 50 mL centrifuge tube and mashed aggressively with the back of a plunger from a sterile 10 mL syringe (Becton Dickinson ...
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bioRxiv - Bioengineering 2024Quote: ... Nunc MaxiSorp plates (Thermo Fisher Scientific) were coated with 100 µL of 1 µg/mL OVA in pH 9.6 carbonate-bicarbonate buffer and incubated on an orbital shaker overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... the cell solution was allowed to drip through a 70 µm cell strainer (Fisher Scientific, Hampton, NH) suspended within a 50 mL centrifuge tube to remove any remaining particulates ...
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bioRxiv - Bioengineering 2024Quote: ... Standard curves were constructed using serial dilutions of λDNA standard (Invitrogen, 25250) in a 10 mM Tris-HCl solution (pH 7.5) ...
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bioRxiv - Bioengineering 2024Quote: qPCR was performed using Dream Taq DNA polymerase (ThermoFisher). Each reaction contains 200 μM dNTP ...
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bioRxiv - Bioengineering 2024Quote: ... mAb1 test samples were prepared at 50 μg/mL in phosphate-buffered saline (Gibco, Thermo Fisher Scientific, MA, USA). Aliquots (99 μL ...
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bioRxiv - Bioengineering 2024Quote: ... Frosty (ThermoFisher, #5100-0001), and moved to a -80°C freezer where they were stored 24-48h before being transitioned to liquid nitrogen for the remainder of time (5-6 days ...
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bioRxiv - Bioengineering 2024Quote: ... goat anti-mouse AlexaFluor 488 (Invitrogen), SPY650-FastAct (Spirochorme) ...
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bioRxiv - Bioengineering 2024Quote: ... were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass, Thermo Scientific) and cultured for 3 days before imaging[46–48] ...
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bioRxiv - Bioengineering 2024Quote: Attenuated total reflectance FTIR spectra were collected using a NicoletTM iS20 FTIR spectrometer (Thermo Fisher Scientific). The atmosphere in the instrument was replaced with a steady stream of nitrogen to avoid capturing atmospheric molecular vibrations ...
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bioRxiv - Bioengineering 2024Quote: ... mouse and human RGCs (Thy1.2-positive cells) were isolated by magnetic microbead-based sorting using the Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit from Invitrogen (catalog number 11465D), following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... and the protein bands were visualized by treating with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific) and the image was captured by ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Bioengineering 2024Quote: ... Nunc Maxisorp 96-well plates (ThermoFisher Scientific) were coated with 100 μL of 1 μg/mL OVA in a 100 mM pH 9.6 carbonate– bicarbonate buffer overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Quantitative PCR was performed using Applied Biosystems QuantStudio™ 5 (Thermo Fisher Scientific). To normalize the gene expression between different samples ...
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bioRxiv - Bioengineering 2024Quote: ... Media samples were thawed and the manufacturer’s protocol for bead staining and analysis on an Attune NxT Cytometer (ThermoFisher, Waltham, MA) was followed ...
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bioRxiv - Bioengineering 2024Quote: ... tissue explants were equilibrated in PBS supplemented with 10 µL/mL protease inhibitor cocktail (HaltTM, Thermo Fisher Scientific) and 10 µL/mL 0.5-M EDTA (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... All cell culture reagents were obtained from Gibco (Invitrogen, Waltham, Massachusetts, USA).
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bioRxiv - Bioengineering 2024Quote: ... pyruvate and GlutaMAX™ Supplement (cat. 10569010, Gibco™). Wild-type CHO-K1 cell lines (CHO-K1 ...