Labshake search
Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... supplemented with 0.1 mM nonessential amino acids (NEAA, Fisher Scientific, cat. #11140076) and 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2024Quote: ... The collected samples were quantified by BCA assay (Thermo Scientific, cat. #23225). Before loading into the gel ...
-
bioRxiv - Immunology 2024Quote: ... Samples were allowed to cool back to room temperature before loading into 12% Bis-Tris 1.0 mm gels (Invitrogen, cat. #NP0321BOX). Proteins were electrophoretically transferred onto nitrocellulose membranes ...
-
bioRxiv - Immunology 2024Quote: ... 167]: the infectious clone plasmid was linearized (MluI) and transcribed in vitro using an mMessage mMachine SP6 transcription kit (Ambion). BHK-21 cells were electroporated with viral RNA ...
-
bioRxiv - Immunology 2024Quote: Stained slides were mounted using prolong diamond antifade (Life Technologies, Carlsbad, California, USA) and scanned using the PerkinElmer Vectra 3.0 system and Vectra software (Akoya Biosciences ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.5 µg of total RNA was reversed transcribed (Applied Biosystems) using the manufacturer’s instructions in a Mastercycler nexus gradient (Eppendorf) ...
-
bioRxiv - Immunology 2024Quote: ... 40 µL of a transfection solution was prepared by combining 2% DharmaFect-4 transfection reagent (Horizon, cat. GP-T-2004-07A) in Opti-MEM (Gibco, cat. 31985070). This was added to 40 µL of a 312.5 nM gRNA library using a Thermofisher Multidrop Reagent Dispenser ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibody against Alexa Fluor 594 (goat anti-rabbit, 1:1000; Thermo Scientific: A11012) was incubated for 2h at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Dried samples were resuspended in LC-MS grade H2O or LC-MS grade 40:40:20 ACN:MeOH:H2O (ACN-Fisher Scientific: A955-4) as loading buffer depending on the LC-MS method.
-
bioRxiv - Cell Biology 2024Quote: ... 1% GlutaMAX-I (Gibco, 35050061), and 10% fetal bovine serum (Novus Biologicals ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein content in the supernatant was quantified using a BCA assay kit (Thermo Fisher Scientific: 23223). Protein samples were heat denatured and separated on 4–12% Bolt Bis-Tris gels (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... 9mM acetic acid (Fisher Scientific: A11350), pH 8.2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... with 10 % fetal calf serum (FCS, Gibco) and Antibiotic-Antimycotic® (Gibco)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... of pregnant mare’s serum gonadotropin (PMSG) (Thermo Fisher, NC1663485). At 48 h post injection ...
-
bioRxiv - Cell Biology 2024Quote: ... containing 10% fetal bovine serum (A3160602; Gibco/Fisher). All cells were authenticated by STR profiling and tested for mycoplasma every six months ...
-
bioRxiv - Cell Biology 2024Quote: ... and a 30 µl droplet of laminin (Invitrogen, 23017-015) was applied ...
-
bioRxiv - Microbiology 2024Quote: ... 100 ug/mL penicillin and streptomycin (Gibco), and 10 μg/mL of puromycin (Gibco).
-
bioRxiv - Immunology 2024Quote: ... cells were re-suspended in 200 μL of cell stimulation cocktail with protein transport inhibitors (Thermo-Fisher Scientific, Cat: 00-4975-03) in CCM for 2 hours at 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... 37.5 mg collagenase type II (Gibco), 1 mL PBS ...
-
bioRxiv - Immunology 2024Quote: ... and Alexa Fluor® 488 Phalloidin (1:1000, Life Technologies, Thermo Fisher Scientific cat. A12379) to stain the actin cytoskeleton ...
-
bioRxiv - Immunology 2024Quote: ... using TaqMan Master Mix (Thermo-Fisher Scientific, Waltham, MA) and primers for the DNA-directed RNA polymerase subunit beta (rpoB ...
-
bioRxiv - Immunology 2024Quote: ... Cells were centrifuged (365 g for 15 min) resuspended in Neurobasal™-A Medium (Gibco – Cat. 10888-022) supplemented with 0.25 mM L-Glutamin ...
-
bioRxiv - Immunology 2024Quote: Assessment of cell viability was performed by staining with 20 nM TO-PRO-3 (Thermo Scientific) or with Fixable Viability Dye (FVD ...
-
bioRxiv - Immunology 2024Quote: ... For H929 cells 50 μM β-mercaptoethanol (Life Technologies) was added and for primary MM the medium was supplemented with 100 ng/ml human recombinant IL-6 (Tebu Bio ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed twice in permeabilization buffer (Invitrogen cat# 00-8333-56), then centrifuged at 1340 x g and stained for intracellular proteins for 50 minutes at room temperature using the following panel ...
-
bioRxiv - Immunology 2024Quote: ... 50 μM β-mercaptoethanol (Life Technologies) and 1% p/s ...
-
bioRxiv - Cell Biology 2024Quote: ... or anti-Tns3 antibody (Invitrogen, PA5-63112) and rocked end-over-end at 4°C overnight ...
-
bioRxiv - Immunology 2024Quote: ... and 100 µg/ml penicillin-streptomycin (p/s, Gibco/Life Technologies). For H929 cells 50 μM β-mercaptoethanol (Life Technologies ...
-
bioRxiv - Immunology 2024Quote: ... the tissue was gently cut into pieces and digested with 200 µL of 2.5% Trypsin (Gibco – Cat. 15090046) at 37°C for 30 min on an orbital shaker in 15 mL plastic tubes ...
-
bioRxiv - Immunology 2024Quote: ... or by staining with CellTrace Violet (Invitrogen) (MM cell lines ...
-
bioRxiv - Immunology 2024Quote: Cells were cultured in Dulbeccós Modified Eagle Medium (DMEM, Life Technologies) (Phoenix-Ampho ...
-
bioRxiv - Immunology 2024Quote: ... Transduced T cells were expanded using 50 U/ml IL-2 and anti CD3/CD28 dynabeads (Thermo Fisher), and BCMA-CAR-expressing cells were selected by treatment with 80 µg/ml neomycin ...
-
bioRxiv - Immunology 2024Quote: ... and Alexa Fluor® 488 Phalloidin (1:1000, Life Technologies, Thermo Fisher Scientific cat. A12379) to stain the actin cytoskeleton ...
-
bioRxiv - Immunology 2024Quote: ... Quantification of the Alexa Fluor® 647 ratio per tetanus toxin was obtained by scanning measurements using the NanoDrop One c device (Thermo Fisher Scientific) with 280 and 650 nm readings ...
-
bioRxiv - Immunology 2024Quote: ... and 40 mg/L gentamicin (10 mg/mL) (Gibco – Cat. 15710-064), and seeded between 0.5×105 – 1×105 per well in 96 well plate coated with 10 µg/mL Poly-D-lysine hydrobromide (Sigma – Cat ...
-
bioRxiv - Immunology 2024Quote: ... The Hoechst dye (1:1000, Life Technologies, Thermo Fisher Scientific cat. #H3570) was used to stain DNA ...
-
bioRxiv - Immunology 2024Quote: ... and biotinylated human BCMA (Bio-connect) with streptavidin-PE (Thermo Fisher). For intracellular staining ...
-
bioRxiv - Immunology 2024Quote: ... and 100 µg/ml penicillin-streptomycin (p/s, Gibco/Life Technologies). For H929 cells 50 μM β-mercaptoethanol (Life Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... All probes were from Molecular Probes (Thermo Fisher Scientific). Cells were then washed 3 times with 0.1 M phosphate buffer (PB ...
-
bioRxiv - Cell Biology 2024Quote: HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% foetal bovine serum at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: ... All probes were from Molecular Probes (Thermo Fisher Scientific). Cells were then washed 3 times with 0.1 M phosphate buffer (PB ...
-
bioRxiv - Cell Biology 2024Quote: ... in Opti-MEM medium (Gibco) as recommended by the manufacturer ...
-
bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine LTX and PLUS reagent (Invitrogen) in Opti-MEM medium (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... To enrich for MCL-1 binding protein immunoprecipitation was preformed using the Dynabeads Protein G IP Kit (Invitrogen) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... Primer and probe sets were generated using the PrimerQuest Tool (Integrated DNA Technologies, IA, USA) and compared with the Multiple Primer Analyzer tool (Thermo-Fisher Scientific, Waltham, MA) (13) ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were counterstained with DAPI and mounted on slides using ProLongTM Gold antifade reagent (ThermoFisher Scientific #P36934).
-
bioRxiv - Immunology 2024Quote: ... or RPMI 1640 GlutaMAX HEPES culture medium (Life Technologies) (L363 ...
-
bioRxiv - Immunology 2024Quote: ... serum-free (Gibco – Cat. 17504044) and 40 mg/L gentamicin (10 mg/mL ...
-
bioRxiv - Immunology 2024Quote: ... The tissues were then digested for 40 minutes in 25 mL digestion media (25 mL RPMI, 12.5 mg dispase (Gibco), 37.5 mg collagenase type II (Gibco) ...
-
bioRxiv - Immunology 2024Quote: ... 0.25 mM GlutaMAX™ (Gibco – Cat. 35050-061), 2% B-27™ (50X) ...