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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The PCR reaction was performed using TaqMan Fast Universal PCR Master Mix (#4367846, Foster City, CA, Applied Biosystems by Life Technologies) and MicroAmp Fast Optical 96-Well reaction plates (#4346906 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad #172-1011 or 172-1019, or ThermoFisher Scientific #31414) diluted in blocking buffer for 1 h at 22 °C ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (1 μg/sample) was used to generate cDNA (SuperScript III Reverse Transcriptase, Invitrogen), which served as template for qPCR using ThermoFisher Scientific TaqMan gene expression assays with the TaqMan Fast Advanced Master Mix ...
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bioRxiv - Neuroscience 2024Quote: ... Data acquisition was controlled by Xcalibur 4.0 and Tune 2.11 software (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with a mixture of 360 μL of 50 mM ammonium bicarbonate (17) and 5 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Neuroscience 2024Quote: ... in StepOnePlus System (ThermoFisher Scientific). Primers used were ...
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bioRxiv - Neuroscience 2024Quote: ... and MicroAmp Fast Optical 96-Well reaction plates (#4346906, Applied Biosystems by Life Technologies) in StepOnePlus System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... they were first washed with PBS (ThermoFisher Scientific #14190144) and then treated with enzyme-free cell dissociation reagent (Millipore Sigma #S-014-B) ...
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bioRxiv - Neuroscience 2024Quote: ... and 1X GlutaMAX (ThermoFisher Scientific #35050061). Cells were cultured at 37 °C in a 5% CO2 humidified incubator and were maintained by routine passaging at a dilution of 1:5 or 1:3 every 3-4 days ...
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bioRxiv - Neuroscience 2024Quote: miRNA level was profiled using miRNA 4.1 Array Strip (#902404, Santa Clara, CA, Affymetrix). The procedure was performed according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... and MicroAmp Fast Optical 96-Well reaction plates (#4346906, Applied Biosystems by Life Technologies) in StepOnePlus System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Both fractions were then used to measure the amyloid beta 1-42 (Aβ1-42) using a specific ELISA kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were run on 10% Bolt Bis-Tris Plus Mini gels (ThermoFisher Scientific #NW00100BOX for 10-well gels ...
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bioRxiv - Neuroscience 2024Quote: ... we used rno-miR-191a-3p (RT:002576, ThermoFisher Scientific), which was selected as the most stable in our experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Using an 18-plex Tandem Mass Tag (TMT-pro) kit (ThermoFisher, Lot# UK297033 and WI336758), 17 channels were allocated for CSF samples with the remaining channel (126 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined with the MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific, United Kingdom).
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bioRxiv - Neuroscience 2024Quote: ... Total proteins were stained with a reversible protein stain kit (Memcode 24580, Thermo Fisher Scientific, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The whole procedure was performed using the GeneAtlas™ instrument from Affymetrix (#00-0393).
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bioRxiv - Neuroscience 2024Quote: ... and rno-miR-674-3p (RT:001956, ThermoFisher Scientific). Data were analyzed using StepOne Software v ...
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bioRxiv - Neuroscience 2024Quote: ... clear-bottom 96-well plates (ThermoFisher Scientific #265301) and then sealed with a clear film (VWR #89134-428) ...
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bioRxiv - Neuroscience 2024Quote: Synthesis of cDNA was performed using TaqMan MicroRNA Reverse Transcription Kit (#4366597, Vilnius, LT, Applied Biosystems by Life Technologies) and nexus gradient Mastercycler (#6331 ...
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bioRxiv - Neuroscience 2024Quote: ... A Vanquish UPLC system (ThermoFisher Scientific) was used to carry out the fractionation ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Neuroscience 2024Quote: ... 8 µl of RNA was used to perform a poly-A-tailing reaction (AF-902134 GA HWS Kit for miRNA Arrays, Affymetrix). Hybridization was done for 20 hours at +48 °C (AF-900454 GeneChip Hybridization Control Kit ...
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bioRxiv - Neuroscience 2024Quote: ... the resultant cell suspensions were quantified using the Countess™ Automated Cell Counter (Invitrogen). A total of 2.5 × 10^6 viable cells were allocated to each well of a U-bottom 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 to 1 mg of cell lysate was digested with PK (ThermoFisher Scientific #EO0491) at a concentration of 50 µg/mL and a PK:protein ratio of 1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... Hybridization was done for 20 hours at +48 °C (AF-900454 GeneChip Hybridization Control Kit, Affymetrix). It was followed by the wash and stain protocol (AF-901910 FlashTagTM Biotin HSR RNA Labeling Kit ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing was performed on an Ion S5XL™ sequencer (Thermo Scientific MA, USA) as we did before (Canedo et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in cell lysates were determined using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23227). For analysis of PrPC levels ...
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bioRxiv - Neuroscience 2024Quote: ... It was followed by the wash and stain protocol (AF-901910 FlashTagTM Biotin HSR RNA Labeling Kit, Affymetrix). The whole procedure was performed using the GeneAtlas™ instrument from Affymetrix (#00-0393).
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bioRxiv - Neuroscience 2024Quote: ... high glucose and pyruvate (Gibco, Life Tech., 31966047), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-rabbit A647 (ThermoFisher-Scientific A32795), donkey anti-rabbit A647 (Invitrogen A31573) ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-rat A647 (Invitrogen A48272), don-key anti-rat 488 (Invitrogen A21208).
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-rabbit A647 (Invitrogen A31573), donkey anti-goat A647 (ThermoFisherScientific A21447) ...
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bioRxiv - Neuroscience 2024Quote: ... in Essential 6 medium (Gibco, A1516401) supplemented with two SMAD pathway inhibitors – dorsomorphin (2.5 μM ...
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bioRxiv - Neuroscience 2024Quote: ... in Essential 8 medium (Gibco, A1517001) and passaged with 0.5 mM EDTA (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Neuroscience 2024Quote: ... cells were collected with TrypLE (Gibco, 16563-029) and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Neuroscience 2024Quote: ... An extracellular bath solution consisting of Hanks’ Balanced Salt Solution without Phenol Red (HBSS; ThermoFisher Scientific) and synaptic blockers were used for all aspects of calcium imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Nitric acid (65% (v/v)) was sourced from Fisher Scientific UK (Loughborough ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged with 0.5 mM EDTA (Gibco, 15575). To coat the six-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then separated on a NuPAGE 4-12% Bis-Tris Protein Gel (Invitrogen) and transferred to a PVDF membrane (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... fibroblast medium was changed to Essential 8 medium (Gibco, A1517001) and cells were fed daily until days 20–22 ...
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bioRxiv - Neuroscience 2024Quote: Western blots were imaged using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific). Western blots were considered successful if the band was visualized at the expected molecular weight (Figure a) ...
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bioRxiv - Neuroscience 2024Quote: ... they were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Neuroscience 2024Quote: ... with added Halt protease and phosphatase inhibitors (Thermo Scientific) for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and penicillin and streptomycin (1:100, Gibco, 15140). The neural medium was supplemented with epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2024Quote: ... The culture medium contained Neurobasal-A (Thermo Fisher, 10888022) supplemented with B27 (2% ...