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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then stripped using Restore™ PLUS Western Blot Stripping Buffer (ThermoFisher Scientific; 46430) and re-blotted with primary antibodies raised in a different species to prevent residual HRP-signal ...
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bioRxiv - Cell Biology 2024Quote: ... 20-30 μg protein was electrophoresed on a 10-20% SDS-PAGE gel (Invitrogen; XP10200BOX), transferred to a PVDF membrane (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... in M199 base media (#11150059, Gibco) on 1 mg/ml gelatin-coated tissue culture flasks ...
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bioRxiv - Cell Biology 2024Quote: ... PCRs were performed using DreamTaq Polymerase (ThermoFisher Scientific; K1081) with primers designed to distinguish between wild type ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to a PVDF membrane (ThermoFisher; 88518), and then blocked in 5% BSA (phosphorylated proteins ...
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bioRxiv - Cancer Biology 2024Quote: 293FT cells were transfected using lipocomplexes (Lipofectamine 2000, ThermoFisher, cat: 11668-019) containing the viral packaging system of second generation as well as the lentiviral construct of interest pCDH-EF1a-mCherry-EGFP-LC3B (RRID:Addgene_170446) ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 cells and all the derivative cell lines were cultured in Dulbecco’s modified Eagle medium (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat ...
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bioRxiv - Cancer Biology 2024Quote: ... and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA, USA).
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bioRxiv - Cell Biology 2024Quote: Mouse Embryonic Fibroblasts (a gift of Yueming Li, MSKCC) were cultured in media composed of DMEM (Thermo Fisher 11965092), Penicillin-Streptomycin/L-glutamine (Thermo Fisher 10378016 ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 cells and all the derivative cell lines were cultured in Dulbecco’s modified Eagle medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) / Nutrient mixture HAM F12 (Biological Industries ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined using a NanoDrop One (Thermo Fisher, ND-ONE-W) using absorbance.
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bioRxiv - Cancer Biology 2024Quote: ... paraffin-embedded samples were sectioned at 4 micron thickness onto Superfrost plus slides (Fisher Scientific). Slides were stained with the Bond Polymer Refine Red Detection kit using a Bond Rxm automated stainer (Leica Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured in Ham’s F-12K medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: Stable transfectant OVCA429 and OVCAR3-dCas9 cells were generated by lipocomplexes (Lipofectamine 2000, ThermoFisher, cat ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 488 conjugate (Invitrogen A11039) and 1:500 goat anti-mouse IgY (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 568 conjugate (Invitrogen A11029).
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bioRxiv - Cancer Biology 2024Quote: ... coli (Stbl3™, ThermoFisher, cat: C737303), and selection by flow cytometry (MoFlo XDP100 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were imaged on a EVOS microscope (Invitrogen) using a GFP filter set and a 10x objective ...
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bioRxiv - Cell Biology 2024Quote: ... The qPCR reaction was carried out using PowerUp SYBR Green (Applied Biosystems) with the Applied Biosystems QuantStudio 3 Real-Time PCR system in a 96-well format ...
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bioRxiv - Cell Biology 2024Quote: ... A slurry of R2 20 µm Poros beads (Life Technologies Corporation) in 5% formic acid and 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2024Quote: ... G1 synchronized U-2OS cells were treated where indicated with 50 μM H2O2 for 15 minutes in Live Cell Imaging Solution (Life Technologies). Cells were allowed to recover up to 4 hours in culture media after removing H2O2 from them ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (11668019; Thermo Fisher) and OptiMEM (31985070 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were mounted in ProlongDiamond with DAPI (Molecular probes). Imaging was performed using a DeltaVision Elite inverted microscope system (Applied Precision) ...
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bioRxiv - Cell Biology 2024Quote: ... Click-it reaction was performed using the Click-iT Plus Picolyl Azide Toolkit (Thermo Fisher Scientific) according to manufacturer’s instructions with modifications ...
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bioRxiv - Cell Biology 2024Quote: ... treated with Alexa Fluor 647-conjugated phalloidin (A22287; Thermo Fisher) at a 1:100 dilution for 60 min ...
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bioRxiv - Cell Biology 2024Quote: ... and OptiMEM (31985070; Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... To remove detergents from the chromatin fractions and to also increase the dynamic range of the analysis the samples were loaded onto a 1.0 mm NuPAGE 4-12% Bis-Tris Gel (Life Technologies). The gel was run for approximately 30 minutes and stained using GelCode Blue Stain Reagent (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... human protein database with common lab contaminants and the sequence of the tagged bait proteins added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... but peptides were gradient eluted from the column directly to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher) using a 155 min acetonitrile gradient from 5 to 35 % B in 120 min followed by a ramp to 45% B in 10 min and 100% B in another 10 min with a hold at 100% B for 10 min (A=2% acetonitrile in 0.5% acetic acid ...
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bioRxiv - Cell Biology 2024Quote: ... were incubated with 10 µg/ml fibronectin (33016015; Thermo Fisher) in PBS at room temperature for 1 hour and rinsed once with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% sodium pyruvate (Gibco). Imaging was performed using a DeltaVision Elite inverted microscope system (Applied Precision) ...
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bioRxiv - Cell Biology 2024Quote: ... 1% v/v non-essential amino acids (11140050; Thermo Fisher Scientific, Waltham, MA), and 1% v/v penicillin-streptomycin solution (15140122 ...
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bioRxiv - Cell Biology 2024Quote: ... Affinity purifications were carried out using MyOne Streptavidin C1 beads (Thermo Fisher Scientific) at 4°C for 4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The gel was run for approximately 30 minutes and stained using GelCode Blue Stain Reagent (Thermo Scientific). The dominant histone H1 bands were excised ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed once with PBS and once with PBS containing 5% BSA before incubating it with Alexa-488 conjugated streptavidin (Thermo Fisher Scientific) at 1:2000 dilution for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μM EdU (Thermo Fisher Scientific, 1149-100), 10 μM MG132 (Peptides International ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% v/v penicillin-streptomycin solution (15140122; Thermo Fisher). The generation of cell lines stably expressing VinTS and VinTS-E1015A-E1021A via lentiviral transduction were described previously33,34,51 ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were gradient eluted directly to an Orbitrap Q Exactive HF-X mass spectrometer (Thermo Fisher) using a 95 min acetonitrile gradient from 5 to 35 % B in 60 min followed by a ramp to 45% B in 10 min and 100% B in another 10 min with a hold at 100% B for 10 min (A=2% acetonitrile in 0.5% acetic acid ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were mounted with ProlongDiamond with DAPI (Molecular probes). Imaging was performed using a DeltaVision Elite inverted microscope system (Applied Precision) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were pulsed with EdU (10 μM final concentration, Thermo Fisher Scientific) for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of EdU was accomplished using the Click-iT Plus EdU Alexa Fluor 488 or 647 Imaging Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... FAMEs were analyzed on a TRACE 1310 gas chromatograph (Thermo Scientific, Les Ulis, France) equipped with a split-splitless injector operated in the splitless mode and a flame-ionization detector ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μL of glacial acetic acid (ACROS Organics AC124040010; Thermo Fisher) was added to a solution of 3-(Trimethoxysilyl ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mM EDTA in PBS (Fisher Scientific). Then ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... was HPLC-grade and purchased from Acros Organics. Ammonium formate (>99% ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... FAMEs were identified by comparing sample retention times to those of commercial standard mixtures (Menhaden oil and Food Industry FAME Mix, Restek) using Xcalibur 2.2 software (Thermo Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... anti-RPL22L1 (Thermo Fisher Scientific, Cat# PA5-63266), anti-MDM4 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS then mounted with ProLong Antifade Gold (ThermoFisher). Images were acquired on using a Plan Fluor 40x Oil DIC H N2 (NA 1.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with PBSTx then stained with secondary antibodies (ThermoFisher, A11001 and A11037). Following PBSTx washes ...